DNA polymerase lambda, a novel DNA repair enzyme in human cells.

Abstract:

DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase ...
DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5'-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the biochemical properties of the polymerization activity of this enzyme are still largely unknown. We have cloned and purified human pol lambda to homogeneity in a soluble and active form, and we present here a biochemical description of its polymerization features. In support of a role in DNA repair, pol lambda inserts nucleotides in a DNA template-dependent manner and is processive in small gaps containing a 5'-phosphate group. These properties, together with its nucleotide insertion fidelity parameters and lack of proofreading activity, indicate that pol lambda is a novel beta-like DNA polymerase. However, the high affinity of pol lambda for dNTPs (37-fold over pol beta) is consistent with its possible involvement in DNA transactions occurring under low cellular levels of dNTPs. This suggests that, despite their similarities, pol beta and pol lambda have nonredundant in vivo functions.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. Molecular Weight 6.8E+04 Dalton Technique: SDS-PAGE
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. 3-5' Exonuclease (proofreading) No
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. Cloned or native Cloned in E. coli
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. Nucleotide Substitution Rate 0.0009 errors/bp Technique: M13mp2 forward mutation assay
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. Full length or truncated Full length
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. Processivity 6bp
Human Pol lamba DNA polymerase lambda, a novel DNA repair enzyme in human cells. KM 0.5uM Reaction: Nucleotide incorporation; Substrate: dNTPs

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