Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates.

Kunkel TA, Pavlov YI, Bebenek K
DNA repair (2003), Volume 2, Page 135
PubMed entry

Abstract:

Human DNA polymerases eta, kappa and iota are template-dependent, ...
Human DNA polymerases eta, kappa and iota are template-dependent, Y-family DNA polymerases that have been implicated in translesion DNA synthesis (TLS) in human cells. Here, we briefly review evidence that these exonuclease-deficient polymerases copy undamaged DNA with very low fidelity and unusual error specificity. Based on the base substitution specificity and other biochemical properties of DNA polymerases eta and iota, we consider the possibility that they participate in specialized DNA transactions that repair damaged DNA and/or generate mutations in the variable regions of immunoglobulin genes.

Polymerases:

Topics:

Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol eta Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. 3-5' Exonuclease (proofreading) No
Human Pol eta Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Template lesions Bypasses Reaction: Nucleotide incorporation; DNA lesion: TT Cyclobutane Pyrimidine Dimer
Human Pol eta Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Nucleotide Substitution Rate 0.0357 errors/bp Technique: Forward mutational
Human Pol eta Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Full length or truncated Full length
Human Pol kappa Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. 3-5' Exonuclease (proofreading) No
Human Pol kappa Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Nucleotide Substitution Rate 0.0066 errors/bp
Human Pol kappa Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Full length or truncated Full length
Human Pol iota Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. 3-5' Exonuclease (proofreading) No
Human Pol iota Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Overall Error Rate 0.0002 errors/bp
Human Pol iota Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Full length or truncated Full length
Human Pol iota Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. Gap Filling Yes

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