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Polymerase: Eco Pol I

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Polymerase Reference Property Result Context
Eco Pol I Kong H1993 Full length or truncated Full length
Eco Pol I Kong H1993 Processivity 97bp
Eco Pol I Kong H1993 KM 2uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Eco Pol I Kunkel TA1984 Nucleotide Substitution Rate 9.01E-06 errors/bp Technique: Reversion (1 mM dNTP)
Eco Pol I Kunkel TA1984 Processivity 3040bp
Eco Pol I McClure WR1975 3-5' Exonuclease (proofreading) Yes
Eco Pol I McClure WR1975 5-3' Exonuclease Yes
Eco Pol I McClure WR1975 Full length or truncated Full length
Eco Pol I McClure WR1975 Specific Activity 1.5E+04 units/mg
Eco Pol I McClure WR1975 KM 1uM Reaction: Nucleotide incorporation; Substrate: dATP
Eco Pol I McClure WR1975 KM 2uM Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Anderson JP2005 3-5' Exonuclease (proofreading) No
Eco Pol I Anderson JP2005 Cloned or native Unspecified
Eco Pol I Anderson JP2005 5-3' Exonuclease Unspecified
Eco Pol I Anderson JP2005 Tagged Unspecified
Eco Pol I Anderson JP2005 Full length or truncated Unspecified
Eco Pol I Anderson JP2005 Application name PCR
Eco Pol I Lackey D1985 3-5' Exonuclease (proofreading) Yes
Eco Pol I Lackey D1985 Cloned or native Native organism
Eco Pol I Lackey D1985 5-3' Exonuclease Yes
Eco Pol I Lackey D1985 Full length or truncated Full length
Eco Pol I Lackey D1985 Processivity 6bp
Eco Pol I Lackey D1985 Specific Activity 25 units/mg
Eco Pol I Tabor S1987 Full length or truncated Full length
Eco Pol I Tabor S1987 Specific Activity 9800 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Tabor S1987 Specific Activity 2.8E+04 units/mg Technique: Polymerase Assay (M13 DNA)
Eco Pol I Matson SW1981 Cloned or native Native organism
Eco Pol I Matson SW1981 5-3' Exonuclease Yes
Eco Pol I Matson SW1981 Tagged No
Eco Pol I Matson SW1981 Full length or truncated Full length
Eco Pol I Matson SW1981 Processivity 20bp
Eco Pol I Matson SW1981 KM 2uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Eco Pol I Matson SW1981 Nick Extension Yes, via strand displacement
Eco Pol I Bonner CA1992 Molecular Weight 1.03E+05 Dalton
Eco Pol I Bonner CA1992 3-5' Exonuclease (proofreading) Yes
Eco Pol I Bonner CA1992 Cloned or native Native organism
Eco Pol I Bonner CA1992 5-3' Exonuclease Yes
Eco Pol I Bonner CA1992 Template lesions Bypasses Reaction: Nucleotide incorporation; DNA lesion: Apurinic/Apyrimidinic (AP) site
Eco Pol I Bonner CA1992 Full length or truncated Full length
Eco Pol I Perler FB1996 Molecular Weight 1.03E+05 Dalton
Eco Pol I Perler FB1996 3-5' Exonuclease (proofreading) Yes
Eco Pol I Perler FB1996 5-3' Exonuclease Yes
Eco Pol I Perler FB1996 Full length or truncated Full length
Eco Pol I Bambara RA1978 Cloned or native Cloned in E. coli
Eco Pol I Bambara RA1978 Tagged No
Eco Pol I Bambara RA1978 Full length or truncated Full length
Eco Pol I Bambara RA1978 Processivity 188bp
Eco Pol I Bambara RA1978 Nick Extension Yes, unspecified mechanism
Eco Pol I Bambara RA1978 Gap Filling Yes
Eco Pol I Travaglini EC1975 Vmax 3.4 /second Reaction: Nucleotide incorporation; Substrate: dGTP
Eco Pol I Travaglini EC1975 Vmax 3.8 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Travaglini EC1975 Vmax 1.32 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Travaglini EC1975 Vmax 2.5 /second Reaction: Nucleotide incorporation; Substrate: dATP
Eco Pol I Travaglini EC1975 Vmax 0.11 /second Reaction: Nucleotide incorporation; Substrate: dCTP
Eco Pol I D'Alessio JM1988 RNase H Yes
Eco Pol I Papanicolaou C1989 Overall Error Rate 7.1E-05 errors/bp Technique: Blue/white screening
Eco Pol I Maor-Shoshani A2000 Overall Error Rate 0.00138 errors/bp
Eco Pol I Maor-Shoshani A2000 Overall Error Rate 0.00078 errors/bp
Eco Pol I Fiala KA2004 Overall Error Rate 0.00017 errors/bp
Eco Pol I Fiala KA2004 Overall Error Rate 0.00053 errors/bp
Eco Pol I Jovin TM1969 Molecular Weight 1.09E+05 Dalton Technique: Sedimentation Equilibrium
Eco Pol I Jovin TM1969 Tagged No
Eco Pol I Bessman MJ1956 Optimal pH pH 8.7
Eco Pol I Bessman MJ1956 Percent/Fold Effect 97 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: NaCl (0.2 mg/mL)
Eco Pol I Bessman MJ1956 Percent/Fold Effect 30 % increase Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 8.7)
Eco Pol I RADDING CM1962 Optimal pH pH 7.5
Eco Pol I Kornberg A1964 Other Important Residues deoxyadenylate and deoxythymidylate [(AT)3 to (AT)7]
Eco Pol I Kornberg A1964 Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (20°C)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 38.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (0µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 99.9 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 95 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (0mM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 94.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (0µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 0 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (.016mM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 94 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: NTPs (0mM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 99.5 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (.005µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 67.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 99.8 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (0µM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (3mM)
Eco Pol I Bessman MJ1958 Percent/Fold Effect 33 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (.005µM)
Eco Pol I Lehman IR1958 Optimal pH pH 8.7
Eco Pol I Lehman IR1958 Percent/Fold Effect 97 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: NaCl (200mM)
Eco Pol I Lehman IR1958 Percent/Fold Effect 90 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: KF (50mM)
Eco Pol I Richardson CC1964 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I Richardson CC1964 5-3' Exonuclease Unspecified
Eco Pol I Richardson CC1964 Optimal pH pH 7.4
Eco Pol I Richardson CC1964 Specific Activity 1.88E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Richardson CC1964 Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7)
Eco Pol I Richardson CC1964 Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7.8)
Eco Pol I Jovin TM1969 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I Jovin TM1969 Cloned or native Cloned in E. coli
Eco Pol I Jovin TM1969 5-3' Exonuclease Yes
Eco Pol I Jovin TM1969 Tagged Yes
Eco Pol I Jovin TM1969 Tag Name Hg(II)
Eco Pol I Jovin TM1969 Percent/Fold Effect 920 % increase Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 98 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 99.9 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 80 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 8.76), Temp (37°C)
Eco Pol I Jovin TM1969 Percent/Fold Effect 42 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), Temp (37°C), pH (pH 6.5)
Eco Pol I Englund PT1969 5-3' Exonuclease Unspecified
Eco Pol I Englund PT1969 Specific Activity 1.8E+04 units/mg
Eco Pol I Kelly RB1970 3-5' Exonuclease (proofreading) Yes
Eco Pol I Kelly RB1970 5-3' Exonuclease Yes
Eco Pol I Kelly RB1970 Optimal pH pH 7.4
Eco Pol I Kelly RB1970 Specific Activity 1.8E+04 units/mg
Eco Pol I Kelly RB1970 Nick Extension Yes, via exo activity
Eco Pol I Kelly RB1970 Strand Displacement Yes
Eco Pol I Huberman JA1970 3-5' Exonuclease (proofreading) Yes
Eco Pol I Huberman JA1970 5-3' Exonuclease Yes
Eco Pol I Huberman JA1970 Extinction Coefficient 9.26x10^4 M^-1 cm^-1 at 280nm
Eco Pol I Huberman JA1970 Kd 4000uM Reaction: Polymerase - NTP interaction; Substrate: 3’-O-methyl-fluorouracil deoxyribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Thymidylyl-(5’,3’)-thymidine 5’-triphosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: Deoxycytidine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 87uM Reaction: Polymerase - NTP interaction; Substrate: 5-fluorouracil deoxyribonucleoside 5'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 167uM Reaction: Polymerase - NTP interaction; Substrate: Purine deoxyribonucleoside 5’-phosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 3700uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxythymidine 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2800uM Reaction: Polymerase - NTP interaction; Substrate: Thymidine 5’-phosphonate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: 2’-deoxyadenosine; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: 3'-O-methyl AMP; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 23uM Reaction: Polymerase - NTP interaction; Substrate: 4’-deoxy-4’-thioadenine ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 69uM Reaction: Polymerase - NTP interaction; Substrate: 6-methylmcrcaptopurinc ribonuclcosidc 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 144uM Reaction: Polymerase - NTP interaction; Substrate: 6-mercaptopurine ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1200uM Reaction: Polymerase - NTP interaction; Substrate: Deoxyadenosine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3900uM Reaction: Polymerase - NTP interaction; Substrate: Adenine-2’-deoxylyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3400uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxy-2’,3’-didehydroadenosine 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3900uM Reaction: Polymerase - NTP interaction; Substrate: Adenine xylonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3100uM Reaction: Polymerase - NTP interaction; Substrate: 5'-fluorouracil arabinonucleoside 5'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 62uM Reaction: Polymerase - NTP interaction; Substrate: Aristeromycin 6'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Deoxythymidine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2300uM Reaction: Polymerase - NTP interaction; Substrate: Adenine arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Adenosine 5’-phosphite; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1700uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxyadenosine 5’-phosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: Thymine arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3700uM Reaction: Polymerase - NTP interaction; Substrate: Uracil arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2700uM Reaction: Polymerase - NTP interaction; Substrate: Adenine-3’-deoxylyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 35uM Reaction: Polymerase - NTP interaction; Substrate: AMP; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Uracil lyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 4200uM Reaction: Polymerase - NTP interaction; Substrate: Barbituric acid ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3400uM Reaction: Polymerase - NTP interaction; Substrate: Thymidine 3’,5’-diphosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1000uM Reaction: Polymerase - NTP interaction; Substrate: Deoxy-guanosine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2300uM Reaction: Polymerase - NTP interaction; Substrate: Uracil xylonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 900uM Reaction: Polymerase - NTP interaction; Substrate: Nicotinamide ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2800uM Reaction: Polymerase - NTP interaction; Substrate: guanosine 5’-methane phosphonate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Deutscher MP1969 KM 600nM Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: Temp (37°C)
Eco Pol I Deutscher MP1969 Ki 0.7mM Reaction: Nucleotide incorporation; Inhibitor: [PP]; Substrate: dNTPs; Experimental conditions: Temp (37°C)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (1mM)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 85 % increase Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (2mM)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 0 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: pH (pH 6.5), Temp (37°C)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 95 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: pH (pH 9), Temp (37°C)
Eco Pol I Setlow P1972 Molecular Weight 1.09E+05 Dalton
Eco Pol I Setlow P1972 3-5' Exonuclease (proofreading) Yes
Eco Pol I Setlow P1972 5-3' Exonuclease Yes
Eco Pol I Setlow P1972 Specific Activity 4500 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Setlow P1972 Percent/Fold Effect 9.75 fold increase Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: TTP (.5mM)
Eco Pol I Setlow P1972 Molecular Weight 1.09E+05 Dalton
Eco Pol I Setlow P1972 3-5' Exonuclease (proofreading) Yes
Eco Pol I Setlow P1972 5-3' Exonuclease Yes
Eco Pol I Setlow P1972 Specific Activity 4500 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Setlow P1972 Extinction Coefficient 0.85 at 278nm

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