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Polymerase: T4

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Polymerase Reference Property Result Context
T4 Fidalgo da Silva E2007 kcat 145 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (with heparin); Experimental conditions: Temp (37°C), Mg++ (8mM)
T4 Fidalgo da Silva E2007 kcat 106 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Multiple Turnover (without heparin); Experimental conditions: Mg++ (8mM), Temp (37°C)
T4 Kong H1993 Processivity 12bp
T4 Kong H1993 KM 2uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
T4 Keohavong P1989 Nucleotide Substitution Rate 3E-06 errors/bp Technique: DGGE
T4 Kunkel TA1984 Nucleotide Substitution Rate 1E-06 errors/bp Technique: Reversion (1 mM dNTP)
T4 Kunkel TA1984 Processivity 1840bp
T4 Creighton S1995 Nucleotide Substitution Rate 1E-06 errors/bp Technique: Gel kinetic assay (dCMP-A mismatch)
T4 Creighton S1995 Nucleotide Substitution Rate 1E-05 errors/bp Technique: Gel kinetic assay (dGMP-A mismatch)
T4 Creighton S1995 Nucleotide Substitution Rate 1E-08 errors/bp Technique: Gel kinetic assay (dAMP-A mismatch)
T4 Creighton S1995 Nucleotide Substitution Rate 0.0002 errors/bp Technique: Gel kinetic assay (dTMP-T mismatch)
T4 Otto MR1998 Methods Featured Fluorescence Spectroscopy
T4 Clayton LK1979 KM 4.6uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Primer extension assay
T4 Clayton LK1979 KM 25.9uM Reaction: Nucleotide incorporation; Substrate: n/a; Technique: Primer extension assay (2AP)
T4 Sinha NK1980 Vmax 500 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Sinha NK1980 Nucleotide incorporation accessory protein(s) gp 32, gp 45, gp 44/62, gp 41
T4 Bedinger P1983 Exonuclease accessory protein(s) gp 45, gp 44/62, gp 32
T4 Hariharan C2005 Methods Featured Fluorescence Spectroscopy
T4 Elisseeva E1999 kcat 200 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA
T4 Beechem JM1998 Methods Featured Fluorescence Spectroscopy
T4 Nossal NG1971 KM 0.04uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nossal NG1971 KM 0.08uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nossal NG1971 Vmax 91 /minute Reaction: 3-5' Exonuclease
T4 Nossal NG1971 Vmax 2.2 /minute Reaction: 3-5' Exonuclease; Technique: Steady State (substrate: E. coli DNA, heat denatured)
T4 Trzemecka A2009 Kd 43nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
T4 Huang WM1972 Molecular Weight 1.15E+05 Dalton
T4 Huang WM1972 3-5' Exonuclease (proofreading) Yes
T4 Huang WM1972 Cloned or native Native organism
T4 Huang WM1972 Tagged No
T4 Huang WM1972 Full length or truncated Full length
T4 Huang WM1972 Specific Activity 2.3E+04 units/mg
T4 Lin TC1994 3-5' Exonuclease (proofreading) Yes
T4 Lin TC1994 KM 1000uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Lin TC1994 KM 7uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Lin TC1994 kcat 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Lin TC1994 kcat 420 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Abdus Sattar AK1996 KM 6.9uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Abdus Sattar AK1996 KM 0.4uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Abdus Sattar AK1996 kcat 65.1 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Abdus Sattar AK1996 kcat 0.16 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Brutlag D1972 Vmax 1100 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Brutlag D1972 Vmax 350 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Brutlag D1972 Vmax 850 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 Brutlag D1972 Vmax 27 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 Lo KY1976 KM 17uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 Lo KY1976 KM 17uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 Lo KY1976 KM 47uM Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 Lo KY1976 Vmax 0.33 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 Lo KY1976 Vmax 0.66 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 Lo KY1976 Vmax 0.15 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Gillin FD1975 KM 1600uM Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.9uM Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 17uM Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.3uM Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 6uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.8uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 190uM Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.3uM Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 60 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 60 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 800 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 1120 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 80 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 160 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 50 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 54 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Topal MD1980 KM 12.3uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template: poly (dG)-(dC))
T4 Topal MD1980 KM 38uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (Template: poly(dA)-(dT))
T4 Topal MD1980 KM 605uM Reaction: Misincorporation; Substrate: dGTP; Technique: Steady State (Template: poly(dA)-(dT))
T4 Topal MD1980 KM 5950uM Reaction: Misincorporation; Substrate: dCTP; Technique: Steady State (Template: poly(dA)-(dT))
T4 Topal MD1980 KM 1850uM Reaction: Misincorporation; Substrate: dATP; Technique: Steady State (Template: poly(dG)-(dC))
T4 Topal MD1980 KM 4940uM Reaction: Misincorporation; Substrate: dTTP; Technique: Steady State (Template: poly(dG)-(dC))
T4 Topal MD1980 KM 6.4uM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (Template: poly(dG)-(dC))
T4 Anderson JP2005 3-5' Exonuclease (proofreading) Yes
T4 Anderson JP2005 Incorporation of non-standard nucleotides Unspecified
T4 Anderson JP2005 Cloned or native Unspecified
T4 Anderson JP2005 5-3' Exonuclease Unspecified
T4 Anderson JP2005 Tagged Unspecified
T4 Anderson JP2005 Full length or truncated Unspecified
T4 Anderson JP2005 Application name PCR
T4 Hershfield MS1973 3-5' Exonuclease (proofreading) Yes
T4 Hershfield MS1973 Cloned or native Native organism
T4 Hershfield MS1973 Tagged No
T4 Hershfield MS1973 Full length or truncated Full length
T4 Wang CX2004 kcat 130 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 Wang CX2004 kcat 91 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Mace DC1984 Processivity 3000bp
T4 Mace DC1984 Nucleotide incorporation accessory protein(s) 44/62 and 45 proteins
T4 Spacciapoli P1994 3-5' Exonuclease (proofreading) Yes
T4 Spacciapoli P1994 Cloned or native Cloned in E. coli
T4 Spacciapoli P1994 Full length or truncated Full length
T4 Spacciapoli P1994 Specific Activity 3039 units/mg
T4 Schrock RD1996 Full length or truncated Full length
T4 Schrock RD1996 Processivity 380bp
T4 Schrock RD1996 Vmax 400 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Carver TE1997 Cloned or native Cloned in E. coli
T4 Carver TE1997 Full length or truncated Full length
T4 Carver TE1997 Processivity 1500bp
T4 Yang J2004 Full length or truncated Full length
T4 Yang J2004 Vmax 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Yang J2004 Kd 145nM Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Hacker KJ1994 3-5' Exonuclease (proofreading) Unspecified
T4 Hacker KJ1994 Cloned or native Cloned in E. coli
T4 Hacker KJ1994 5-3' Exonuclease Unspecified
T4 Hacker KJ1994 Full length or truncated Full length
T4 Hacker KJ1994 Processivity 46bp
T4 Nossal NG1980 Extension from RNA primer Yes
T4 Liu CC1980 Extension from RNA primer Yes
T4 Cha TA1986 Extension from RNA primer Yes
T4 Nossal NG1987 Extension from RNA primer Yes
T4 Nossal NG1974 Nick Extension Yes, unspecified mechanism
T4 Bhagwat M2001 RNase H Yes
T4 Khare V2002 3-5' Exonuclease (proofreading) Yes
T4 Khare V2002 5-3' Exonuclease No
T4 Khare V2002 Extension from RNA primer Yes
T4 Nossal NG2007 3-5' Exonuclease (proofreading) Yes
T4 Nossal NG2007 5-3' Exonuclease No
T4 Nossal NG2007 Extension from RNA primer Yes
T4 Ripley LS1986 Frameshift Error Rate 3E-07 errors/bp
T4 Goulian M1968 Molecular Weight 1.12E+05 Dalton
T4 Goulian M1968 3-5' Exonuclease (proofreading) Yes
T4 Goulian M1968 5-3' Exonuclease No
T4 Goulian M1968 Full length or truncated Full length
T4 Goulian M1968 Optimal pH pH 8.5
T4 Goulian M1968 Specific Activity 3.3E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
T4 Goulian M1968 Strand Displacement No
T4 Goulian M1968 Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (6mM)
T4 Goulian M1968 Percent/Fold Effect 75 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (.1mM)
T4 Goulian M1968 Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: NTPs (0mM)
T4 Goulian M1968 Percent/Fold Effect 49 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0mM), CTP (0mM), TTP (0mM)
T4 Goulian M1968 Percent/Fold Effect 81 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0mM)
T4 Goulian M1968 Percent/Fold Effect 96 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0.2mM), TTP (0mM)
T4 Goulian M1968 Gap Filling Yes
T4 Kroutil LC1998 3-5' Exonuclease (proofreading) Yes
T4 Kroutil LC1998 5-3' Exonuclease Unspecified
T4 Kroutil LC1998 Frameshift Error Rate 1.2E-06 errors/bp Technique: M13mp2 forward mutation assay
T4 Kroutil LC1998 Nucleotide Substitution Rate 9.4E-07 errors/bp Technique: M13mp2 forward mutation assay
T4 Kroutil LC1998 Nucleotide Substitution Rate 3.3E-06 errors/bp Technique: M13mp2 opal codon reversion assay
T4 Kelly RB1970 5-3' Exonuclease No
T4 Drake JW1991 Overall Error Rate 2E-08 errors/bp Technique: rII+ reversion

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.