T7

From bacteriophage T7 (aliases:T7 DNA polymerase, phage T7)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for T7.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

T7 is a family A enzyme from bacteriophage T7.

Selected Properties for T7:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (13) Yes (2) 3.7e-07-1.5e-03 errors/bp (3) 2.2e-06-4.5e-02 errors/bp (4) 2.6e-07-6.7e-06 errors/bp (2)
Year Authors Title Results by property
2017 TP Hoekstra, M Depken, SN Lin, J Cabanas-Danés, P Gross, RT Dame, EJ Peterman, GJ Wuite Hoekstra TP2017
2013 Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Gerhard Wagner, Charles C Richardson Akabayov B2013
2013 Leena Maddukuri, Sarah C Shuck, Robert Lawton Eoff, Linlin Zhao, Carmelo J Rizzo, F Peter Guengerich, Lawrence J Marnett Maddukuri L2013 Template lesions,All properties
2012 Arkadiusz W Kulczyk, Charles C Richardson Kulczyk AW2012
2012 Ngoc Quang Tran, Seung-Joo Lee, Barak Akabayov, Donald E Johnson, Charles C Richardson Tran NQ2012
2012 Arkadiusz W Kulczyk, Barak Akabayov, Seung-Joo Lee, Mihnea Bostina, Steven A Berkowitz, Charles C Richardson Kulczyk AW2012 Other accessory protein(s),All properties
2011 Vladimir V Didenko Didenko VV2011
2011 Zhinan Jin, Kenneth A Johnson Jin Z2011
2011 Gayatri Patel, Daniel S Johnson, Bo Sun, Manjula Pandey, Xiong Yu, Edward H Egelman, Michelle D Wang, Smita S Patel Patel G2011
2011 M Hogg, AE Sauer-Eriksson, Erik Johansson Hogg M2011
2011 Huidong Zhang, Seung-Joo Lee, B Zhu, Ngoc Quang Tran, Stanley Tabor, Charles C Richardson Zhang H2011
2011 Katrin Viikov, Priit Väljamäe, Juhan Sedman Viikov K2011 Processivity,Strand Displacement,All properties
2011 B Zhu, Seung-Joo Lee, Charles C Richardson Zhu B2011 Nucleotide incorporation accessory protein(s),All properties
2010 Ajit K Satapathy, Anna B Kochaniak, Sourav Mukherjee, DJ Crampton, Antoine M van Oijen, Charles C Richardson Satapathy AK2010
2010 Candice M Etson, Samir M Hamdan, Charles C Richardson, Antoine M van Oijen Etson CM2010
2010 Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Stanley Tabor, Arkadiusz W Kulczyk, Charles C Richardson Akabayov B2010
2010 Felix Olasagasti, Kate R Lieberman, Seico Benner, Gerald M Cherf, Joseph M Dahl, David W Deamer, Mark Akeson Olasagasti F2010
2010 Linda J Reha-Krantz Reha-Krantz LJ2010
2009 Yu-Chih Tsai, Zhinan Jin, Kenneth A Johnson Tsai YC2009
2009 Sharmistha Ghosh, Boriana Marintcheva, Masateru Takahashi, Charles C Richardson Ghosh S2009
2009 Brett Gyarfas, Felix Olasagasti, Seico Benner, Daniel R Garalde, Kate R Lieberman, Mark Akeson Gyarfas B2009
2009 Liming Wang, Suse Broyde, Yongqing Zhang Wang L2009
2009 Huidong Zhang, Urban Bren, Ivan D Kozekov, Carmelo J Rizzo, Donald F Stec, F Peter Guengerich Zhang H2009
2009 Kiyonobu Karata, Antonio E Vidal, Roger Woodgate Karata K2009
2009 Seung-Joo Lee, Kajal Chowdhury, Stanley Tabor, Charles C Richardson Lee SJ2009
2009 Boriana Marintcheva, Udi Qimron, Yao Yu, Stanley Tabor, Charles C Richardson, Charles C Richardson Marintcheva B2009
2008 Ngoc Quang Tran, Lisa F Rezende, Udi Qimron, Charles C Richardson, Stanley Tabor Tran NQ2008
2008 Michael D Scholle, Bridget S Banach, Samir M Hamdan, Charles C Richardson, Brian K Kay Scholle MD2008
2008 Sharmistha Ghosh, Samir M Hamdan, Timothy E Cook, Charles C Richardson Ghosh S2008
2008 Lakshminarayan M Iyer, Saraswathi Abhiman, L Aravind Iyer LM2008
2008 Linda J Reha-Krantz Reha-Krantz LJ2008
2007 Donald E Johnson, Masateru Takahashi, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson Johnson DE2007
2007 Vincent J Cannistraro, John-Stephen Taylor Cannistraro VJ2007
2007 Guobin Luo, Michael Wang, William H Konigsberg, X Sunney Xie Luo G2007
2007 Christian Castro, Eric D Smidansky, Kenneth R Maksimchuk, Jamie J Arnold, Victoria S Korneeva, Matthias Götte, William H Konigsberg, Craig E Cameron Castro C2007
2007 Samir M Hamdan, Donald E Johnson, Nathan A Tanner, Jong-Bong Lee, Udi Qimron, Stanley Tabor, Antoine M van Oijen, Charles C Richardson Hamdan SM2007
2006 Joyce Chiu, Daniel Tillett, Paul E March Chiu J2006
2006 Boriana Marintcheva, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson Marintcheva B2006
2006 Yu-Chih Tsai, Kenneth A Johnson Tsai YC2006
2006 Tae Woo Kim, Luis G Brieba, Tom Ellenberger, Eric T Kool Kim TW2006
2006 Joyce Chiu, Daniel Tillett, Paul E March Chiu J2006
2006 Seung-Joo Lee, Boriana Marintcheva, Samir M Hamdan, Charles C Richardson Lee SJ2006
2006 Scott D McCulloch, Thomas A Kunkel McCulloch SD2006
2006 Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada, Shigeyuki Yokoyama Hirao I2006
2005 Samir M Hamdan, Boriana Marintcheva, Timothy E Cook, Seung-Joo Lee, Stanley Tabor, Charles C Richardson Hamdan SM2005
2005 Hong Zang, Thomas M Harris, F Peter Guengerich Zang H2005
2005 Hong Zang, Thomas M Harris, F Peter Guengerich Zang H2005
2005 Seung-Joo Lee, Charles C Richardson Lee SJ2005
2005 Jan Florián, Myron F Goodman, Arieh Warshel Florián J2005
2005 William A Beard, Samuel H Wilson Beard WA2005
2005 Marina Schlicke, Susanne Brakmann Schlicke M2005
2005 Luis G Brieba, Robert J Kokoska, Katarzyna Bebenek, Thomas A Kunkel, Tom Ellenberger Brieba LG2005
2005 Matthew Hogg, Susan S Wallace, Sylvie Doublié Hogg M2005
2004 Kevin A Fiala, Zucai Suo Fiala KA2004 Overall Error Rate,All properties
2004 Yingqian Li, Shuchismita Dutta, Sylvie Doublié, Hussam Moh'd Bdour, John-Stephen Taylor, Tom Ellenberger Li Y2004
2004 Luis G Brieba, Brandt F Eichman, Robert J Kokoska, Sylvie Doublié, Thomas A Kunkel, Tom Ellenberger Brieba LG2004
2004 Vincent J Cannistraro, John-Stephen Taylor Cannistraro VJ2004
2004 Shuchismita Dutta, Yingqian Li, Donald E Johnson, Leonid Dzantiev, Charles C Richardson, Louis J Romano, Tom Ellenberger Dutta S2004
2004 DJ Crampton, S Guo, Donald E Johnson, Charles C Richardson Crampton DJ2004
2004 Jaya K Kumar, Erica T Chiu, Stanley Tabor, Charles C Richardson Kumar JK2004
2004 Masato Kato, Takuhiro Ito, Gerhard Wagner, Tom Ellenberger Kato M2004
2003 Ichiro Hirao, Tsuneo Mitsui, Michiko Kimoto, Yoko Harada, Shigeyuki Yokoyama Hirao I2003
2003 Robert E Johnson, José Trincao, Aneel K Aggarwal, Satya Prakash, Louise Prakash Johnson RE2003
2003 Zheng-Guo He, Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson He ZG2003
2003 William A Beard, Samuel H Wilson Beard WA2003
2003 Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson Rezende LF2003
2003 Edel M Hyland, Lisa F Rezende, Charles C Richardson Hyland EM2003
2003 Netai C Singha, Alexios Vlamis-Gardikas, Arne Holmgren Singha NC2003
2003 Natasha Paul, Vishal C Nashine, Geoffrey Hoops, Peiming Zhang, Jie Zhou, Donald E Bergstrom, V Jo Davisson Paul N2003
2002 Cuihua Liu, Craig T Martin Liu C2002
2002 Lisa F Rezende, Thomas Hollis, Tom Ellenberger, Charles C Richardson Rezende LF2002
2002 Joonsoo Lee, Paul D Chastain, Jack D Griffith, Charles C Richardson Lee J2002
2002 Adrienne M Woodside, F Peter Guengerich Woodside AM2002
2002 Adrienne M Woodside, F Peter Guengerich Woodside AM2002
2001 Jaya K Kumar, R Kremsdorf, Stanley Tabor, Charles C Richardson Kumar JK2001
2001 Thomas Hollis, J M Stattel, D S Walther, Charles C Richardson, Tom Ellenberger Hollis T2001
2001 Graciella Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, Ghislain Opdenakker Andrei G2001
2001 Emmanuelle Delagoutte, Peter H von Hippel Delagoutte E2001
2001 Farid A Kadyrov, John W Drake Kadyrov FA2001
2001 T C Lin, C X Wang, Catherine M Joyce, William H Konigsberg Lin TC2001
2001 R A Perlow, Suse Broyde Perlow RA2001
2000 T Y Tseng, David N Frick, Charles C Richardson Tseng TY2000
2000 L M Wentzell, A Maxwell Wentzell LM2000
2000 Kajal Chowdhury, Stanley Tabor, Charles C Richardson Chowdhury K2000
2000 G J Wuite, Steven B Smith, M Young, D Keller, Carlos Bustamante Wuite GJ2000 Full length or truncated,Processivity,3-5' exo,Vmax,All properties
2000 L Sun, Michael Wang, Eric T Kool, John-Stephen Taylor Sun L2000
1999 Miguel de Vega, Luis Blanco, Margarita Salas de Vega M1999 3-5' exo,All properties
1999 D Canceill, Enrique Viguera, S Dusko Ehrlich Canceill D1999
1999 Zucai Suo, S J Lippard, Kenneth A Johnson Suo Z1999
1999 S Guo, Stanley Tabor, Charles C Richardson Guo S1999
1998 Zucai Suo, Kenneth A Johnson Suo Z1998
1998 P Keohavong, R Shukla, A Melacrinos, B W Day, Linda J Reha-Krantz Keohavong P1998
1998 M Falkenberg, P Elias, I. Robert Lehman Falkenberg M1998
1998 F Hill, David Loakes, D M Brown Hill F1998
1998 Sylvie Doublié, Tom Ellenberger Doublié S1998
1998 Sylvie Doublié, Stanley Tabor, A M Long, Charles C Richardson, Tom Ellenberger Doublié S1998
1998 K Park, Zeger Debyser, Stanley Tabor, Charles C Richardson, Jack D Griffith Park K1998
1998 D Kong, Charles C Richardson Kong D1998
1998 David N Frick, K Baradaran, Charles C Richardson Frick DN1998
1998 L L Furge, F Peter Guengerich Furge LL1998
1998 S W Graves, Allison A Johnson, Kenneth A Johnson Graves SW1998 Processivity,Kd,Vmax,All properties
1997 S M Notarnicola, H L Mulcahy, Joonsoo Lee, Charles C Richardson Notarnicola SM1997
1997 T Kusakabe, Charles C Richardson Kusakabe T1997
1997 E Bedford, Stanley Tabor, Charles C Richardson Bedford E1997 Specific Activity,Processivity,3-5' exo,All properties
1997 X M Yang, Charles C Richardson Yang XM1997 Full length or truncated,Processivity,All properties
1996 S Wiemann, A Schilke, S Rechmann, J Zimmermann, H Voss, W Ansorge Wiemann S1996
1996 J S Himawan, Charles C Richardson Himawan JS1996
1996 J W Brandis, S G Edwards, Kenneth A Johnson Brandis JW1996
1996 F B Perler, Sandeep Kumar, H Kong Perler FB1996 Full length or truncated,3-5' exo,Vmax,All properties
1995 M N Rigler, Louis J Romano Rigler MN1995
1995 Stanley Tabor, Charles C Richardson Tabor S1995
1994 Thomas A Kunkel, Smita S Patel, Kenneth A Johnson Kunkel TA1994 Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Frameshift Error Rate,Nucleotide Substitution Rate,Nucleotide incorporation accessory protein(s),All properties
1994 L C Bridgewater, F C Manning, E S Woo, Steven R Patierno Bridgewater LC1994
1994 J E Lindsley, Robert P P Fuchs Lindsley JE1994
1994 M Thakar, A Bilenko, W J Becktel Thakar M1994
1994 J Ji, Jean-Sébastien Hoffmann, L Loeb Ji J1994
1994 Zeger Debyser, Stanley Tabor, Charles C Richardson Debyser Z1994
1994 M Bodescot, O Brison Bodescot M1994
1994 M Bodescot, O Brison Bodescot M1994
1994 M J Donlin, Kenneth A Johnson Donlin MJ1994
1993 S M Wurgler, Charles C Richardson Wurgler SM1993
1993 H Kong, R B Kucera, William E Jack Kong H1993 Processivity,KM,All properties
1993 D R Koehler, P C Hanawalt Koehler DR1993
1992 L G Lee, C R Connell, S L Woo, R D Cheng, B F McArdle, Carl W Fuller, N D Halloran, R K Wilson Lee LG1992
1992 Unknown1992
1992 J S Himawan, Charles C Richardson Himawan JS1992
1992 Y T Kim, Stanley Tabor, J E Churchich, Charles C Richardson Kim YT1992
1992 Carl W Fuller Fuller CW1992
1991 I Wong, Smita S Patel, Kenneth A Johnson Wong I1991
1991 I Slaby, A Holmgren Slaby I1991
1991 Smita S Patel, I Wong, Kenneth A Johnson Patel SS1991 Cloned or native,Full length or truncated,Processivity,3-5' exo,KM,Kd,Vmax,All properties
1991 P Mattila, J Korpela, T Tenkanen, K Pitkänen Mattila P1991 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties
1991 M J Donlin, Smita S Patel, Kenneth A Johnson Donlin MJ1991
1990 D B Reardon, C A Bigger, A Dipple Reardon DB1990
1990 B S Strauss, Jianhong Wang Strauss BS1990
1990 Stanley Tabor, Charles C Richardson Tabor S1990
1989 I Slaby, A Holmgren Slaby I1989
1989 J A Bernstein, Charles C Richardson Bernstein JA1989
1989 Katarzyna Bebenek, Thomas A Kunkel Bebenek K1989
1989 Stanley Tabor, Charles C Richardson Tabor S1989
1989 J Sahm, E Turkington, D LaPointe, Bernard Strauss Sahm J1989
1989 Stanley Tabor, Charles C Richardson Tabor S1989
1988 S D Rabkin, Charles C Richardson Rabkin SD1988
1988 H Nakai, Charles C Richardson Nakai H1988
1988 T Z Su, M R el-Gewely Su TZ1988
1988 T W Myers, Louis J Romano Myers TW1988
1987 Stanley Tabor, Charles C Richardson Tabor S1987
1987 Stanley Tabor, Charles C Richardson Tabor S1987
1987 Stanley Tabor, H E Huber, Charles C Richardson Tabor S1987 Cloned or native,Tagged,Full length or truncated,Specific Activity,Processivity,Vmax,All properties
1987 H E Huber, Stanley Tabor, Charles C Richardson Huber HE1987
1986 M L Michaels, M S Lee, Louis J Romano Michaels ML1986
1986 H Nakai, Charles C Richardson Nakai H1986
1985 C W Fuller, Charles C Richardson Fuller CW1985
1985 C W Fuller, Charles C Richardson Fuller CW1985
1985 Stanley Tabor, Charles C Richardson Tabor S1985
1984 I Slaby, B Lind, A Holmgren Slaby I1984
1983 R L Lechner, M J Engler, Charles C Richardson Lechner RL1983 Cloned or native,Tagged,Full length or truncated,Specific Activity,Nick Extension,3-5' exo,Other accessory protein(s),All properties
1983 P Bedinger, B M Alberts Bedinger P1983
1983 M J Engler, R L Lechner, Charles C Richardson Engler MJ1983 Cloned or native,Tagged,Full length or truncated,Specific Activity,Extension from RNA primer,Nick Extension,5-3' exo,Nucleotide Substitution Rate,Molecular Weight,Other accessory protein(s),All properties
1983 S W Matson, Stanley Tabor, Charles C Richardson Matson SW1983
1983 S W Matson, Charles C Richardson Matson SW1983
1983 M J Engler, Charles C Richardson Engler MJ1983
1983 S Adler, P Modrich Adler S1983 Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties
1983 R L Lechner, Charles C Richardson Lechner RL1983
1982 H Randahl Randahl H1982
1982 H Randahl, I Slabý, A Holmgren Randahl H1982
1981 Louis J Romano, F Tamanoi, Charles C Richardson Romano LJ1981
1981 Stanley Tabor, Charles C Richardson Tabor S1981
1981 A Holmgren, G B Kallis, B Nordström Holmgren A1981
1981 B Nordström, H Randahl, I Slaby, A Holmgren Nordström B1981 Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties
1979 S Adler, P Modrich Adler S1979 Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,3-5' Exo Specific Activity,Other accessory protein(s),All properties
1979 K Hori, D F Mark, Charles C Richardson Hori K1979 Cloned or native,Tagged,Full length or truncated,Specific Activity,Isoelectric Point,3-5' exo,KM,3-5' Exo Specific Activity,Other accessory protein(s),All properties
1979 Louis J Romano, Charles C Richardson Romano LJ1979
1979 K Hori, D F Mark, Charles C Richardson Hori K1979
1978 R Kolodner, Y Masamune, J E LeClerc, Charles C Richardson Kolodner R1978
1978 R Kolodner, Charles C Richardson Kolodner R1978
1977 R Kolodner, Charles C Richardson Kolodner R1977
1976 D F Mark, Charles C Richardson Mark DF1976
1975 David C Hinkle, Charles C Richardson Hinkle DC1975
1975 P Modrich, Charles C Richardson Modrich P1975 Cloned or native,Tagged,Full length or truncated,Specific Activity,Other accessory protein(s),All properties
1975 P Modrich, Charles C Richardson Modrich P1975 Cloned or native,Tagged,Full length or truncated,Specific Activity,Molecular Weight,All properties
1971 Paul T Englund Englund PT1971
1971 P Grippo, Charles C Richardson Grippo P1971 Cloned or native,Tagged,Full length or truncated,3-5' exo,All properties
1971 Y Masamune, Charles C Richardson Masamune Y1971
Protein sequence:
DNA sequence:
All Polymerases | 122 Results

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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