T7

Welcome to the Polymerase Page
This page presents all the information in Polbase for T7.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
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links to this help text. The question mark icon is used everywhere to indicate that help is available.
Selected Properties for T7: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
---|---|---|---|---|---|
Yes (13) | Yes (2) | 3.7e-07-1.5e-03 errors/bp (3) | 2.2e-06-4.5e-02 errors/bp (4) | 2.6e-07-6.7e-06 errors/bp (2) |
Year | Authors | Title | Results by property |
---|---|---|---|
2017 | TP Hoekstra, M Depken, SN Lin, J Cabanas-Danés, P Gross, RT Dame, EJ Peterman, GJ Wuite | Hoekstra TP2017 | |
2013 | Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Gerhard Wagner, Charles C Richardson | Akabayov B2013 | |
2013 | Leena Maddukuri, Sarah C Shuck, Robert Lawton Eoff, Linlin Zhao, Carmelo J Rizzo, F Peter Guengerich, Lawrence J Marnett | Maddukuri L2013 | Template lesions,All properties |
2012 | Arkadiusz W Kulczyk, Charles C Richardson | Kulczyk AW2012 | |
2012 | Ngoc Quang Tran, Seung-Joo Lee, Barak Akabayov, Donald E Johnson, Charles C Richardson | Tran NQ2012 | |
2012 | Arkadiusz W Kulczyk, Barak Akabayov, Seung-Joo Lee, Mihnea Bostina, Steven A Berkowitz, Charles C Richardson | Kulczyk AW2012 | Other accessory protein(s),All properties |
2011 | Vladimir V Didenko | Didenko VV2011 | |
2011 | Zhinan Jin, Kenneth A Johnson | Jin Z2011 | |
2011 | Gayatri Patel, Daniel S Johnson, Bo Sun, Manjula Pandey, Xiong Yu, Edward H Egelman, Michelle D Wang, Smita S Patel | Patel G2011 | |
2011 | M Hogg, AE Sauer-Eriksson, Erik Johansson | Hogg M2011 | |
2011 | Huidong Zhang, Seung-Joo Lee, B Zhu, Ngoc Quang Tran, Stanley Tabor, Charles C Richardson | Zhang H2011 | |
2011 | Katrin Viikov, Priit Väljamäe, Juhan Sedman | Viikov K2011 | Processivity,Strand Displacement,All properties |
2011 | B Zhu, Seung-Joo Lee, Charles C Richardson | Zhu B2011 | Nucleotide incorporation accessory protein(s),All properties |
2010 | Ajit K Satapathy, Anna B Kochaniak, Sourav Mukherjee, DJ Crampton, Antoine M van Oijen, Charles C Richardson | Satapathy AK2010 | |
2010 | Candice M Etson, Samir M Hamdan, Charles C Richardson, Antoine M van Oijen | Etson CM2010 | |
2010 | Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Stanley Tabor, Arkadiusz W Kulczyk, Charles C Richardson | Akabayov B2010 | |
2010 | Felix Olasagasti, Kate R Lieberman, Seico Benner, Gerald M Cherf, Joseph M Dahl, David W Deamer, Mark Akeson | Olasagasti F2010 | |
2010 | Linda J Reha-Krantz | Reha-Krantz LJ2010 | |
2009 | Yu-Chih Tsai, Zhinan Jin, Kenneth A Johnson | Tsai YC2009 | |
2009 | Sharmistha Ghosh, Boriana Marintcheva, Masateru Takahashi, Charles C Richardson | Ghosh S2009 | |
2009 | Brett Gyarfas, Felix Olasagasti, Seico Benner, Daniel R Garalde, Kate R Lieberman, Mark Akeson | Gyarfas B2009 | |
2009 | Liming Wang, Suse Broyde, Yongqing Zhang | Wang L2009 | |
2009 | Huidong Zhang, Urban Bren, Ivan D Kozekov, Carmelo J Rizzo, Donald F Stec, F Peter Guengerich | Zhang H2009 | |
2009 | Kiyonobu Karata, Antonio E Vidal, Roger Woodgate | Karata K2009 | |
2009 | Seung-Joo Lee, Kajal Chowdhury, Stanley Tabor, Charles C Richardson | Lee SJ2009 | |
2009 | Boriana Marintcheva, Udi Qimron, Yao Yu, Stanley Tabor, Charles C Richardson, Charles C Richardson | Marintcheva B2009 | |
2008 | Ngoc Quang Tran, Lisa F Rezende, Udi Qimron, Charles C Richardson, Stanley Tabor | Tran NQ2008 | |
2008 | Michael D Scholle, Bridget S Banach, Samir M Hamdan, Charles C Richardson, Brian K Kay | Scholle MD2008 | |
2008 | Sharmistha Ghosh, Samir M Hamdan, Timothy E Cook, Charles C Richardson | Ghosh S2008 | |
2008 | Lakshminarayan M Iyer, Saraswathi Abhiman, L Aravind | Iyer LM2008 | |
2008 | Linda J Reha-Krantz | Reha-Krantz LJ2008 | |
2007 | Donald E Johnson, Masateru Takahashi, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson | Johnson DE2007 | |
2007 | Vincent J Cannistraro, John-Stephen Taylor | Cannistraro VJ2007 | |
2007 | Guobin Luo, Michael Wang, William H Konigsberg, X Sunney Xie | Luo G2007 | |
2007 | Christian Castro, Eric D Smidansky, Kenneth R Maksimchuk, Jamie J Arnold, Victoria S Korneeva, Matthias Götte, William H Konigsberg, Craig E Cameron | Castro C2007 | |
2007 | Samir M Hamdan, Donald E Johnson, Nathan A Tanner, Jong-Bong Lee, Udi Qimron, Stanley Tabor, Antoine M van Oijen, Charles C Richardson | Hamdan SM2007 | |
2006 | Joyce Chiu, Daniel Tillett, Paul E March | Chiu J2006 | |
2006 | Boriana Marintcheva, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson | Marintcheva B2006 | |
2006 | Yu-Chih Tsai, Kenneth A Johnson | Tsai YC2006 | |
2006 | Tae Woo Kim, Luis G Brieba, Tom Ellenberger, Eric T Kool | Kim TW2006 | |
2006 | Joyce Chiu, Daniel Tillett, Paul E March | Chiu J2006 | |
2006 | Seung-Joo Lee, Boriana Marintcheva, Samir M Hamdan, Charles C Richardson | Lee SJ2006 | |
2006 | Scott D McCulloch, Thomas A Kunkel | McCulloch SD2006 | |
2006 | Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada, Shigeyuki Yokoyama | Hirao I2006 | |
2005 | Samir M Hamdan, Boriana Marintcheva, Timothy E Cook, Seung-Joo Lee, Stanley Tabor, Charles C Richardson | Hamdan SM2005 | |
2005 | Hong Zang, Thomas M Harris, F Peter Guengerich | Zang H2005 | |
2005 | Hong Zang, Thomas M Harris, F Peter Guengerich | Zang H2005 | |
2005 | Seung-Joo Lee, Charles C Richardson | Lee SJ2005 | |
2005 | Jan Florián, Myron F Goodman, Arieh Warshel | Florián J2005 | |
2005 | William A Beard, Samuel H Wilson | Beard WA2005 | |
2005 | Marina Schlicke, Susanne Brakmann | Schlicke M2005 | |
2005 | Luis G Brieba, Robert J Kokoska, Katarzyna Bebenek, Thomas A Kunkel, Tom Ellenberger | Brieba LG2005 | |
2005 | Matthew Hogg, Susan S Wallace, Sylvie Doublié | Hogg M2005 | |
2004 | Kevin A Fiala, Zucai Suo | Fiala KA2004 | Overall Error Rate,All properties |
2004 | Yingqian Li, Shuchismita Dutta, Sylvie Doublié, Hussam Moh'd Bdour, John-Stephen Taylor, Tom Ellenberger | Li Y2004 | |
2004 | Luis G Brieba, Brandt F Eichman, Robert J Kokoska, Sylvie Doublié, Thomas A Kunkel, Tom Ellenberger | Brieba LG2004 | |
2004 | Vincent J Cannistraro, John-Stephen Taylor | Cannistraro VJ2004 | |
2004 | Shuchismita Dutta, Yingqian Li, Donald E Johnson, Leonid Dzantiev, Charles C Richardson, Louis J Romano, Tom Ellenberger | Dutta S2004 | |
2004 | DJ Crampton, S Guo, Donald E Johnson, Charles C Richardson | Crampton DJ2004 | |
2004 | Jaya K Kumar, Erica T Chiu, Stanley Tabor, Charles C Richardson | Kumar JK2004 | |
2004 | Masato Kato, Takuhiro Ito, Gerhard Wagner, Tom Ellenberger | Kato M2004 | |
2003 | Ichiro Hirao, Tsuneo Mitsui, Michiko Kimoto, Yoko Harada, Shigeyuki Yokoyama | Hirao I2003 | |
2003 | Robert E Johnson, José Trincao, Aneel K Aggarwal, Satya Prakash, Louise Prakash | Johnson RE2003 | |
2003 | Zheng-Guo He, Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson | He ZG2003 | |
2003 | William A Beard, Samuel H Wilson | Beard WA2003 | |
2003 | Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson | Rezende LF2003 | |
2003 | Edel M Hyland, Lisa F Rezende, Charles C Richardson | Hyland EM2003 | |
2003 | Netai C Singha, Alexios Vlamis-Gardikas, Arne Holmgren | Singha NC2003 | |
2003 | Natasha Paul, Vishal C Nashine, Geoffrey Hoops, Peiming Zhang, Jie Zhou, Donald E Bergstrom, V Jo Davisson | Paul N2003 | |
2002 | Cuihua Liu, Craig T Martin | Liu C2002 | |
2002 | Lisa F Rezende, Thomas Hollis, Tom Ellenberger, Charles C Richardson | Rezende LF2002 | |
2002 | Joonsoo Lee, Paul D Chastain, Jack D Griffith, Charles C Richardson | Lee J2002 | |
2002 | Adrienne M Woodside, F Peter Guengerich | Woodside AM2002 | |
2002 | Adrienne M Woodside, F Peter Guengerich | Woodside AM2002 | |
2001 | Jaya K Kumar, R Kremsdorf, Stanley Tabor, Charles C Richardson | Kumar JK2001 | |
2001 | Thomas Hollis, J M Stattel, D S Walther, Charles C Richardson, Tom Ellenberger | Hollis T2001 | |
2001 | Graciella Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, Ghislain Opdenakker | Andrei G2001 | |
2001 | Emmanuelle Delagoutte, Peter H von Hippel | Delagoutte E2001 | |
2001 | Farid A Kadyrov, John W Drake | Kadyrov FA2001 | |
2001 | T C Lin, C X Wang, Catherine M Joyce, William H Konigsberg | Lin TC2001 | |
2001 | R A Perlow, Suse Broyde | Perlow RA2001 | |
2000 | T Y Tseng, David N Frick, Charles C Richardson | Tseng TY2000 | |
2000 | L M Wentzell, A Maxwell | Wentzell LM2000 | |
2000 | Kajal Chowdhury, Stanley Tabor, Charles C Richardson | Chowdhury K2000 | |
2000 | G J Wuite, Steven B Smith, M Young, D Keller, Carlos Bustamante | Wuite GJ2000 | Full length or truncated,Processivity,3-5' exo,Vmax,All properties |
2000 | L Sun, Michael Wang, Eric T Kool, John-Stephen Taylor | Sun L2000 | |
1999 | Miguel de Vega, Luis Blanco, Margarita Salas | de Vega M1999 | 3-5' exo,All properties |
1999 | D Canceill, Enrique Viguera, S Dusko Ehrlich | Canceill D1999 | |
1999 | Zucai Suo, S J Lippard, Kenneth A Johnson | Suo Z1999 | |
1999 | S Guo, Stanley Tabor, Charles C Richardson | Guo S1999 | |
1998 | Zucai Suo, Kenneth A Johnson | Suo Z1998 | |
1998 | P Keohavong, R Shukla, A Melacrinos, B W Day, Linda J Reha-Krantz | Keohavong P1998 | |
1998 | M Falkenberg, P Elias, I. Robert Lehman | Falkenberg M1998 | |
1998 | F Hill, David Loakes, D M Brown | Hill F1998 | |
1998 | Sylvie Doublié, Tom Ellenberger | Doublié S1998 | |
1998 | Sylvie Doublié, Stanley Tabor, A M Long, Charles C Richardson, Tom Ellenberger | Doublié S1998 | |
1998 | K Park, Zeger Debyser, Stanley Tabor, Charles C Richardson, Jack D Griffith | Park K1998 | |
1998 | D Kong, Charles C Richardson | Kong D1998 | |
1998 | David N Frick, K Baradaran, Charles C Richardson | Frick DN1998 | |
1998 | L L Furge, F Peter Guengerich | Furge LL1998 | |
1998 | S W Graves, Allison A Johnson, Kenneth A Johnson | Graves SW1998 | Processivity,Kd,Vmax,All properties |
1997 | S M Notarnicola, H L Mulcahy, Joonsoo Lee, Charles C Richardson | Notarnicola SM1997 | |
1997 | T Kusakabe, Charles C Richardson | Kusakabe T1997 | |
1997 | E Bedford, Stanley Tabor, Charles C Richardson | Bedford E1997 | Specific Activity,Processivity,3-5' exo,All properties |
1997 | X M Yang, Charles C Richardson | Yang XM1997 | Full length or truncated,Processivity,All properties |
1996 | S Wiemann, A Schilke, S Rechmann, J Zimmermann, H Voss, W Ansorge | Wiemann S1996 | |
1996 | J S Himawan, Charles C Richardson | Himawan JS1996 | |
1996 | J W Brandis, S G Edwards, Kenneth A Johnson | Brandis JW1996 | |
1996 | F B Perler, Sandeep Kumar, H Kong | Perler FB1996 | Full length or truncated,3-5' exo,Vmax,All properties |
1995 | M N Rigler, Louis J Romano | Rigler MN1995 | |
1995 | Stanley Tabor, Charles C Richardson | Tabor S1995 | |
1994 | Thomas A Kunkel, Smita S Patel, Kenneth A Johnson | Kunkel TA1994 | Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Frameshift Error Rate,Nucleotide Substitution Rate,Nucleotide incorporation accessory protein(s),All properties |
1994 | L C Bridgewater, F C Manning, E S Woo, Steven R Patierno | Bridgewater LC1994 | |
1994 | J E Lindsley, Robert P P Fuchs | Lindsley JE1994 | |
1994 | M Thakar, A Bilenko, W J Becktel | Thakar M1994 | |
1994 | J Ji, Jean-Sébastien Hoffmann, L Loeb | Ji J1994 | |
1994 | Zeger Debyser, Stanley Tabor, Charles C Richardson | Debyser Z1994 | |
1994 | M Bodescot, O Brison | Bodescot M1994 | |
1994 | M Bodescot, O Brison | Bodescot M1994 | |
1994 | M J Donlin, Kenneth A Johnson | Donlin MJ1994 | |
1993 | S M Wurgler, Charles C Richardson | Wurgler SM1993 | |
1993 | H Kong, R B Kucera, William E Jack | Kong H1993 | Processivity,KM,All properties |
1993 | D R Koehler, P C Hanawalt | Koehler DR1993 | |
1992 | L G Lee, C R Connell, S L Woo, R D Cheng, B F McArdle, Carl W Fuller, N D Halloran, R K Wilson | Lee LG1992 | |
1992 | Unknown1992 | ||
1992 | J S Himawan, Charles C Richardson | Himawan JS1992 | |
1992 | Y T Kim, Stanley Tabor, J E Churchich, Charles C Richardson | Kim YT1992 | |
1992 | Carl W Fuller | Fuller CW1992 | |
1991 | I Wong, Smita S Patel, Kenneth A Johnson | Wong I1991 | |
1991 | I Slaby, A Holmgren | Slaby I1991 | |
1991 | Smita S Patel, I Wong, Kenneth A Johnson | Patel SS1991 | Cloned or native,Full length or truncated,Processivity,3-5' exo,KM,Kd,Vmax,All properties |
1991 | P Mattila, J Korpela, T Tenkanen, K Pitkänen | Mattila P1991 | 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties |
1991 | M J Donlin, Smita S Patel, Kenneth A Johnson | Donlin MJ1991 | |
1990 | D B Reardon, C A Bigger, A Dipple | Reardon DB1990 | |
1990 | B S Strauss, Jianhong Wang | Strauss BS1990 | |
1990 | Stanley Tabor, Charles C Richardson | Tabor S1990 | |
1989 | I Slaby, A Holmgren | Slaby I1989 | |
1989 | J A Bernstein, Charles C Richardson | Bernstein JA1989 | |
1989 | Katarzyna Bebenek, Thomas A Kunkel | Bebenek K1989 | |
1989 | Stanley Tabor, Charles C Richardson | Tabor S1989 | |
1989 | J Sahm, E Turkington, D LaPointe, Bernard Strauss | Sahm J1989 | |
1989 | Stanley Tabor, Charles C Richardson | Tabor S1989 | |
1988 | S D Rabkin, Charles C Richardson | Rabkin SD1988 | |
1988 | H Nakai, Charles C Richardson | Nakai H1988 | |
1988 | T Z Su, M R el-Gewely | Su TZ1988 | |
1988 | T W Myers, Louis J Romano | Myers TW1988 | |
1987 | Stanley Tabor, Charles C Richardson | Tabor S1987 | |
1987 | Stanley Tabor, Charles C Richardson | Tabor S1987 | |
1987 | Stanley Tabor, H E Huber, Charles C Richardson | Tabor S1987 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Processivity,Vmax,All properties |
1987 | H E Huber, Stanley Tabor, Charles C Richardson | Huber HE1987 | |
1986 | M L Michaels, M S Lee, Louis J Romano | Michaels ML1986 | |
1986 | H Nakai, Charles C Richardson | Nakai H1986 | |
1985 | C W Fuller, Charles C Richardson | Fuller CW1985 | |
1985 | C W Fuller, Charles C Richardson | Fuller CW1985 | |
1985 | Stanley Tabor, Charles C Richardson | Tabor S1985 | |
1984 | I Slaby, B Lind, A Holmgren | Slaby I1984 | |
1983 | R L Lechner, M J Engler, Charles C Richardson | Lechner RL1983 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Nick Extension,3-5' exo,Other accessory protein(s),All properties |
1983 | P Bedinger, B M Alberts | Bedinger P1983 | |
1983 | M J Engler, R L Lechner, Charles C Richardson | Engler MJ1983 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Extension from RNA primer,Nick Extension,5-3' exo,Nucleotide Substitution Rate,Molecular Weight,Other accessory protein(s),All properties |
1983 | S W Matson, Stanley Tabor, Charles C Richardson | Matson SW1983 | |
1983 | S W Matson, Charles C Richardson | Matson SW1983 | |
1983 | M J Engler, Charles C Richardson | Engler MJ1983 | |
1983 | S Adler, P Modrich | Adler S1983 | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
1983 | R L Lechner, Charles C Richardson | Lechner RL1983 | |
1982 | H Randahl | Randahl H1982 | |
1982 | H Randahl, I Slabý, A Holmgren | Randahl H1982 | |
1981 | Louis J Romano, F Tamanoi, Charles C Richardson | Romano LJ1981 | |
1981 | Stanley Tabor, Charles C Richardson | Tabor S1981 | |
1981 | A Holmgren, G B Kallis, B Nordström | Holmgren A1981 | |
1981 | B Nordström, H Randahl, I Slaby, A Holmgren | Nordström B1981 | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
1979 | S Adler, P Modrich | Adler S1979 | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
1979 | K Hori, D F Mark, Charles C Richardson | Hori K1979 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Isoelectric Point,3-5' exo,KM,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
1979 | Louis J Romano, Charles C Richardson | Romano LJ1979 | |
1979 | K Hori, D F Mark, Charles C Richardson | Hori K1979 | |
1978 | R Kolodner, Y Masamune, J E LeClerc, Charles C Richardson | Kolodner R1978 | |
1978 | R Kolodner, Charles C Richardson | Kolodner R1978 | |
1977 | R Kolodner, Charles C Richardson | Kolodner R1977 | |
1976 | D F Mark, Charles C Richardson | Mark DF1976 | |
1975 | David C Hinkle, Charles C Richardson | Hinkle DC1975 | |
1975 | P Modrich, Charles C Richardson | Modrich P1975 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Other accessory protein(s),All properties |
1975 | P Modrich, Charles C Richardson | Modrich P1975 | Cloned or native,Tagged,Full length or truncated,Specific Activity,Molecular Weight,All properties |
1971 | Paul T Englund | Englund PT1971 | |
1971 | P Grippo, Charles C Richardson | Grippo P1971 | Cloned or native,Tagged,Full length or truncated,3-5' exo,All properties |
1971 | Y Masamune, Charles C Richardson | Masamune Y1971 |
Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
---|---|---|---|---|---|
T7 (exo-) | No (1) | ||||
T7 E480D | |||||
T7 Y526F | |||||
T7 Y530F | |||||
T7 K587N,K589N,R590N,R591N | |||||
T7 (no thioreoxin) | |||||
Sequenase | No (1) | 3.4e-05-4.4e-05 errors/bp (2) | 4.4e-05-5.4e-05 errors/bp (2) | ||
T7 (purified without EDTA) | Yes (2) | 4.5e-02 errors/bp (1) |
Protein information: Uniprot P00581 search for T7 at UniProt
Sequence information: NCBI nucleotides European nucleotide archive