T7
From
bacteriophage T7
(aliases:T7 DNA polymerase, phage T7)
Welcome to the Polymerase Page
This page presents all the information in Polbase for T7.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
T7
is a family A enzyme
from
bacteriophage T7.
Selected Properties for T7: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Yes (13) | Yes (2) | 3.7e-07-1.5e-03 errors/bp (3) | 2.2e-06-4.5e-02 errors/bp (4) | 2.6e-07-6.7e-06 errors/bp (2) |
- References (184)
- Structures (15)
- Sequences (1)
- Mutants (8)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
|---|---|---|---|
| 2017 | TP Hoekstra, M Depken, SN Lin, J Cabanas-Danés, P Gross, RT Dame, EJ Peterman, GJ Wuite | Switching between Exonucleolysis and ... | |
| 2013 | Leena Maddukuri, Sarah C Shuck, Robert Lawton Eoff, Linlin Zhao, Carmelo J Rizzo, F Peter Guengerich, Lawrence J Marnett | Replication, Repair and Translesion P... | Template lesions,All properties |
| 2013 | Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Gerhard Wagner, Charles C Richardson | Impact of macromolecular crowding on ... | |
| 2012 | Ngoc Quang Tran, Seung-Joo Lee, Barak Akabayov, Donald E Johnson, Charles C Richardson | Thioredoxin, the Processivity Factor,... | |
| 2012 | Arkadiusz W Kulczyk, Barak Akabayov, Seung-Joo Lee, Mihnea Bostina, Steven A Berkowitz, Charles C Richardson | An Interaction between DNA Polymerase... | Other accessory protein(s),All properties |
| 2012 | Arkadiusz W Kulczyk, Charles C Richardson | Molecular interactions in the priming... | |
| 2011 | Gayatri Patel, Daniel S Johnson, Bo Sun, Manjula Pandey, Xiong Yu, Edward H Egelman, Michelle D Wang, Smita S Patel | A257T linker region mutant of T7 heli... | |
| 2011 | Zhinan Jin, Kenneth A Johnson | Role of a GAG hinge in the nucleotide... | |
| 2011 | M Hogg, AE Sauer-Eriksson, Erik Johansson | Promiscuous DNA synthesis by human DN... | |
| 2011 | Vladimir V Didenko | In situ labeling of DNA breaks and ap... | |
| 2011 | B Zhu, Seung-Joo Lee, Charles C Richardson | Bypass of a nick by the replisome of ... | Nucleotide incorporation accessory protein(s),All properties |
| 2011 | Katrin Viikov, Priit Väljamäe, Juhan Sedman | Yeast mitochondrial DNA polymerase is... | Processivity,Strand Displacement,All properties |
| 2011 | Huidong Zhang, Seung-Joo Lee, B Zhu, Ngoc Quang Tran, Stanley Tabor, Charles C Richardson | Helicase-DNA polymerase interaction i... | |
| 2010 | Candice M Etson, Samir M Hamdan, Charles C Richardson, Antoine M van Oijen | Thioredoxin suppresses microscopic ho... | |
| 2010 | Ajit K Satapathy, Anna B Kochaniak, Sourav Mukherjee, DJ Crampton, Antoine M van Oijen, Charles C Richardson | Residues in the central beta-hairpin ... | |
| 2010 | Barak Akabayov, Sabine R Akabayov, Seung-Joo Lee, Stanley Tabor, Arkadiusz W Kulczyk, Charles C Richardson | Conformational dynamics of bacterioph... | |
| 2010 | Linda J Reha-Krantz | DNA polymerase proofreading: Multiple... | |
| 2010 | Felix Olasagasti, Kate R Lieberman, Seico Benner, Gerald M Cherf, Joseph M Dahl, David W Deamer, Mark Akeson | Replication of individual DNA molecul... | |
| 2009 | Huidong Zhang, Urban Bren, Ivan D Kozekov, Carmelo J Rizzo, Donald F Stec, F Peter Guengerich | Steric and electrostatic effects at t... | |
| 2009 | Seung-Joo Lee, Kajal Chowdhury, Stanley Tabor, Charles C Richardson | Rescue of bacteriophage T7 DNA polyme... | |
| 2009 | Liming Wang, Suse Broyde, Yongqing Zhang | Polymerase-tailored variations in the... | |
| 2009 | Yu-Chih Tsai, Zhinan Jin, Kenneth A Johnson | Site-specific labeling of T7 DNA poly... | |
| 2009 | Sharmistha Ghosh, Boriana Marintcheva, Masateru Takahashi, Charles C Richardson | C-terminal phenylalanine of bacteriop... | |
| 2009 | Kiyonobu Karata, Antonio E Vidal, Roger Woodgate | Construction of a circular single-str... | |
| 2009 | Boriana Marintcheva, Udi Qimron, Yao Yu, Stanley Tabor, Charles C Richardson, Charles C Richardson | Mutations in the gene 5 DNA polymeras... | |
| 2009 | Brett Gyarfas, Felix Olasagasti, Seico Benner, Daniel R Garalde, Kate R Lieberman, Mark Akeson | Mapping the position of DNA polymeras... | |
| 2008 | Sharmistha Ghosh, Samir M Hamdan, Timothy E Cook, Charles C Richardson | Interactions of Escherichia coli thio... | |
| 2008 | Lakshminarayan M Iyer, Saraswathi Abhiman, L Aravind | A new family of polymerases related t... | |
| 2008 | Ngoc Quang Tran, Lisa F Rezende, Udi Qimron, Charles C Richardson, Stanley Tabor | Gene 1.7 of bacteriophage T7 confers ... | |
| 2008 | Michael D Scholle, Bridget S Banach, Samir M Hamdan, Charles C Richardson, Brian K Kay | Peptide ligands specific to the oxidi... | |
| 2008 | Linda J Reha-Krantz | Recent patents of gene sequences rela... | |
| 2007 | Donald E Johnson, Masateru Takahashi, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson | Exchange of DNA polymerases at the re... | |
| 2007 | Samir M Hamdan, Donald E Johnson, Nathan A Tanner, Jong-Bong Lee, Udi Qimron, Stanley Tabor, Antoine M van Oijen, Charles C Richardson | Dynamic DNA helicase-DNA polymerase i... | |
| 2007 | Guobin Luo, Michael Wang, William H Konigsberg, X Sunney Xie | Single-molecule and ensemble fluoresc... | |
| 2007 | Vincent J Cannistraro, John-Stephen Taylor | Ability of polymerase eta and T7 DNA ... | |
| 2007 | Christian Castro, Eric D Smidansky, Kenneth R Maksimchuk, Jamie J Arnold, Victoria S Korneeva, Matthias Götte, William H Konigsberg, Craig E Cameron | Two proton transfers in the transitio... | |
| 2006 | Boriana Marintcheva, Samir M Hamdan, Seung-Joo Lee, Charles C Richardson | Essential residues in the C terminus ... | |
| 2006 | Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada, Shigeyuki Yokoyama | An unnatural hydrophobic base pair sy... | |
| 2006 | Scott D McCulloch, Thomas A Kunkel | Multiple solutions to inefficient les... | |
| 2006 | Seung-Joo Lee, Boriana Marintcheva, Samir M Hamdan, Charles C Richardson | The C-terminal residues of bacterioph... | |
| 2006 | Joyce Chiu, Daniel Tillett, Paul E March | Mutation of Phe102 to Ser in the carb... | |
| 2006 | Joyce Chiu, Daniel Tillett, Paul E March | Coexpression of the subunits of T7 DN... | |
| 2006 | Yu-Chih Tsai, Kenneth A Johnson | A new paradigm for DNA polymerase spe... | |
| 2006 | Tae Woo Kim, Luis G Brieba, Tom Ellenberger, Eric T Kool | Functional evidence for a small and r... | |
| 2005 | Hong Zang, Thomas M Harris, F Peter Guengerich | Kinetics of nucleotide incorporation ... | |
| 2005 | Samir M Hamdan, Boriana Marintcheva, Timothy E Cook, Seung-Joo Lee, Stanley Tabor, Charles C Richardson | A unique loop in T7 DNA polymerase me... | |
| 2005 | William A Beard, Samuel H Wilson | Syn-full behavior by T7 DNA polymerase. | |
| 2005 | Matthew Hogg, Susan S Wallace, Sylvie Doublié | Bumps in the road: how replicative DN... | |
| 2005 | Jan Florián, Myron F Goodman, Arieh Warshel | Computer simulations of protein funct... | |
| 2005 | Luis G Brieba, Robert J Kokoska, Katarzyna Bebenek, Thomas A Kunkel, Tom Ellenberger | A lysine residue in the fingers subdo... | |
| 2005 | Seung-Joo Lee, Charles C Richardson | Acidic residues in the nucleotide-bin... | |
| 2005 | Hong Zang, Thomas M Harris, F Peter Guengerich | Kinetics of nucleotide incorporation ... | |
| 2005 | Marina Schlicke, Susanne Brakmann | Expression and purification of histid... | |
| 2004 | Jaya K Kumar, Erica T Chiu, Stanley Tabor, Charles C Richardson | A unique region in bacteriophage t7 D... | |
| 2004 | Vincent J Cannistraro, John-Stephen Taylor | DNA-thumb interactions and processivi... | |
| 2004 | Kevin A Fiala, Zucai Suo | Pre-steady-state kinetic studies of t... | Overall Error Rate,All properties |
| 2004 | Luis G Brieba, Brandt F Eichman, Robert J Kokoska, Sylvie Doublié, Thomas A Kunkel, Tom Ellenberger | Structural basis for the dual coding ... | |
| 2004 | Yingqian Li, Shuchismita Dutta, Sylvie Doublié, Hussam Moh'd Bdour, John-Stephen Taylor, Tom Ellenberger | Nucleotide insertion opposite a cis-s... | |
| 2004 | Masato Kato, Takuhiro Ito, Gerhard Wagner, Tom Ellenberger | A molecular handoff between bacteriop... | |
| 2004 | Shuchismita Dutta, Yingqian Li, Donald E Johnson, Leonid Dzantiev, Charles C Richardson, Louis J Romano, Tom Ellenberger | Crystal structures of 2-acetylaminofl... | |
| 2004 | DJ Crampton, S Guo, Donald E Johnson, Charles C Richardson | The arginine finger of bacteriophage ... | |
| 2003 | Netai C Singha, Alexios Vlamis-Gardikas, Arne Holmgren | Real-time kinetics of the interaction... | |
| 2003 | Natasha Paul, Vishal C Nashine, Geoffrey Hoops, Peiming Zhang, Jie Zhou, Donald E Bergstrom, V Jo Davisson | DNA polymerase template interactions ... | |
| 2003 | Robert E Johnson, José Trincao, Aneel K Aggarwal, Satya Prakash, Louise Prakash | Deoxynucleotide triphosphate binding ... | |
| 2003 | Zheng-Guo He, Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson | The carboxyl-terminal domain of bacte... | |
| 2003 | Lisa F Rezende, Smaranda Willcox, Jack D Griffith, Charles C Richardson | A single-stranded DNA-binding protein... | |
| 2003 | Edel M Hyland, Lisa F Rezende, Charles C Richardson | The DNA binding domain of the gene 2.... | |
| 2003 | William A Beard, Samuel H Wilson | Structural insights into the origins ... | |
| 2003 | Ichiro Hirao, Tsuneo Mitsui, Michiko Kimoto, Yoko Harada, Shigeyuki Yokoyama | An unnatural base pair for efficient ... | |
| 2002 | Adrienne M Woodside, F Peter Guengerich | Misincorporation and stalling at O(6)... | |
| 2002 | Joonsoo Lee, Paul D Chastain, Jack D Griffith, Charles C Richardson | Lagging strand synthesis in coordinat... | |
| 2002 | Lisa F Rezende, Thomas Hollis, Tom Ellenberger, Charles C Richardson | Essential amino acid residues in the ... | |
| 2002 | Cuihua Liu, Craig T Martin | Promoter clearance by T7 RNA polymera... | |
| 2002 | Adrienne M Woodside, F Peter Guengerich | Effect of the O6 substituent on misin... | |
| 2001 | Jaya K Kumar, R Kremsdorf, Stanley Tabor, Charles C Richardson | A Mutation in the gene-encoding bacte... | |
| 2001 | Graciella Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, Ghislain Opdenakker | Evaluating phenotype and genotype of ... | |
| 2001 | Emmanuelle Delagoutte, Peter H von Hippel | Molecular mechanisms of the functiona... | |
| 2001 | Thomas Hollis, J M Stattel, D S Walther, Charles C Richardson, Tom Ellenberger | Structure of the gene 2.5 protein, a ... | |
| 2001 | Farid A Kadyrov, John W Drake | Conditional coupling of leading-stran... | |
| 2001 | T C Lin, C X Wang, Catherine M Joyce, William H Konigsberg | 3'-5' Exonucleolytic activity of DNA ... | |
| 2001 | R A Perlow, Suse Broyde | Evading the proofreading machinery of... | |
| 2000 | G J Wuite, Steven B Smith, M Young, D Keller, Carlos Bustamante | Single-molecule studies of the effect... | Full length or truncated,Processivity,3-5' exo,Vmax,All properties |
| 2000 | T Y Tseng, David N Frick, Charles C Richardson | Characterization of a novel DNA prima... | |
| 2000 | L M Wentzell, A Maxwell | The complex of DNA gyrase and quinolo... | |
| 2000 | Kajal Chowdhury, Stanley Tabor, Charles C Richardson | A unique loop in the DNA-binding crev... | |
| 2000 | L Sun, Michael Wang, Eric T Kool, John-Stephen Taylor | Pyrene nucleotide as a mechanistic pr... | |
| 1999 | Miguel de Vega, Luis Blanco, Margarita Salas | Processive proofreading and the spati... | 3-5' exo,All properties |
| 1999 | D Canceill, Enrique Viguera, S Dusko Ehrlich | Replication slippage of different DNA... | |
| 1999 | S Guo, Stanley Tabor, Charles C Richardson | The linker region between the helicas... | |
| 1999 | Zucai Suo, S J Lippard, Kenneth A Johnson | Single d(GpG)/cis-diammineplatinum(II... | |
| 1998 | S W Graves, Allison A Johnson, Kenneth A Johnson | Expression, purification, and initial... | Processivity,Kd,Vmax,All properties |
| 1998 | M Falkenberg, P Elias, I. Robert Lehman | The herpes simplex virus type 1 helic... | |
| 1998 | P Keohavong, R Shukla, A Melacrinos, B W Day, Linda J Reha-Krantz | Effects of bulky polycyclic aromatic ... | |
| 1998 | F Hill, David Loakes, D M Brown | Polymerase recognition of synthetic o... | |
| 1998 | K Park, Zeger Debyser, Stanley Tabor, Charles C Richardson, Jack D Griffith | Formation of a DNA loop at the replic... | |
| 1998 | Sylvie Doublié, Tom Ellenberger | The mechanism of action of T7 DNA pol... | |
| 1998 | David N Frick, K Baradaran, Charles C Richardson | An N-terminal fragment of the gene 4 ... | |
| 1998 | D Kong, Charles C Richardson | Role of the acidic carboxyl-terminal ... | |
| 1998 | Sylvie Doublié, Stanley Tabor, A M Long, Charles C Richardson, Tom Ellenberger | Crystal structure of a bacteriophage ... | |
| 1998 | L L Furge, F Peter Guengerich | Pre-steady-state kinetics of nucleoti... | |
| 1998 | Zucai Suo, Kenneth A Johnson | Selective inhibition of HIV-1 reverse... | |
| 1997 | X M Yang, Charles C Richardson | Amino acid changes in a unique sequen... | Full length or truncated,Processivity,All properties |
| 1997 | S M Notarnicola, H L Mulcahy, Joonsoo Lee, Charles C Richardson | The acidic carboxyl terminus of the b... | |
| 1997 | E Bedford, Stanley Tabor, Charles C Richardson | The thioredoxin binding domain of bac... | Specific Activity,Processivity,3-5' exo,All properties |
| 1997 | T Kusakabe, Charles C Richardson | Gene 4 DNA primase of bacteriophage T... | |
| 1996 | J W Brandis, S G Edwards, Kenneth A Johnson | Slow rate of phosphodiester bond form... | |
| 1996 | F B Perler, Sandeep Kumar, H Kong | Thermostable DNA polymerases. | Full length or truncated,3-5' exo,Vmax,All properties |
| 1996 | S Wiemann, A Schilke, S Rechmann, J Zimmermann, H Voss, W Ansorge | Reducing "double sequences" in automa... | |
| 1996 | J S Himawan, Charles C Richardson | Amino acid residues critical for the ... | |
| 1995 | Stanley Tabor, Charles C Richardson | A single residue in DNA polymerases o... | |
| 1995 | M N Rigler, Louis J Romano | Differences in the mechanism of stimu... | |
| 1994 | M J Donlin, Kenneth A Johnson | Mutants affecting nucleotide recognit... | |
| 1994 | M Bodescot, O Brison | T7 DNA polymerase requires unusual re... | |
| 1994 | M Bodescot, O Brison | Efficient second-strand cDNA synthesi... | |
| 1994 | Thomas A Kunkel, Smita S Patel, Kenneth A Johnson | Error-prone replication of repeated D... | Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Frameshift Error Rate,Nucleotide Substitution Rate,Nucleotide incorporation accessory protein(s),All properties |
| 1994 | Zeger Debyser, Stanley Tabor, Charles C Richardson | Coordination of leading and lagging s... | |
| 1994 | M Thakar, A Bilenko, W J Becktel | Osmolyte mediation of T7 DNA polymera... | |
| 1994 | J E Lindsley, Robert P P Fuchs | Use of single-turnover kinetics to st... | |
| 1994 | J Ji, Jean-Sébastien Hoffmann, L Loeb | Mutagenicity and pausing of HIV rever... | |
| 1994 | L C Bridgewater, F C Manning, E S Woo, Steven R Patierno | DNA polymerase arrest by adducted tri... | |
| 1993 | H Kong, R B Kucera, William E Jack | Characterization of a DNA polymerase ... | Processivity,KM,All properties |
| 1993 | S M Wurgler, Charles C Richardson | DNA binding properties of the deoxygu... | |
| 1993 | D R Koehler, P C Hanawalt | Digestion of damaged DNA by the T7 DN... | |
| 1992 | J S Himawan, Charles C Richardson | Genetic analysis of the interaction b... | |
| 1992 | Carl W Fuller | Modified T7 DNA polymerase for DNA se... | |
| 1992 | L G Lee, C R Connell, S L Woo, R D Cheng, B F McArdle, Carl W Fuller, N D Halloran, R K Wilson | DNA sequencing with dye-labeled termi... | |
| 1992 | Y T Kim, Stanley Tabor, J E Churchich, Charles C Richardson | Interactions of gene 2.5 protein and ... | |
| 1992 | DNA Replication | ||
| 1991 | I Wong, Smita S Patel, Kenneth A Johnson | An induced-fit kinetic mechanism for ... | |
| 1991 | I Slaby, A Holmgren | Rapid isolation of homogeneous cloned... | |
| 1991 | Smita S Patel, I Wong, Kenneth A Johnson | Pre-steady-state kinetic analysis of ... | Cloned or native,Full length or truncated,Processivity,3-5' exo,KM,Kd,Vmax,All properties |
| 1991 | P Mattila, J Korpela, T Tenkanen, K Pitkänen | Fidelity of DNA synthesis by the Ther... | 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties |
| 1991 | M J Donlin, Smita S Patel, Kenneth A Johnson | Kinetic partitioning between the exon... | |
| 1990 | B S Strauss, Jianhong Wang | Role of DNA polymerase 3'----5' exonu... | |
| 1990 | Stanley Tabor, Charles C Richardson | DNA sequence analysis with a modified... | |
| 1990 | D B Reardon, C A Bigger, A Dipple | DNA polymerase action on bulky deoxyg... | |
| 1989 | Stanley Tabor, Charles C Richardson | Selective inactivation of the exonucl... | |
| 1989 | Stanley Tabor, Charles C Richardson | Effect of manganese ions on the incor... | |
| 1989 | Katarzyna Bebenek, Thomas A Kunkel | The use of native T7 DNA polymerase f... | |
| 1989 | I Slaby, A Holmgren | Thioredoxin reductase-dependent insul... | |
| 1989 | J Sahm, E Turkington, D LaPointe, Bernard Strauss | Mutation induced in vitro on a C-8 gu... | |
| 1989 | J A Bernstein, Charles C Richardson | Characterization of the helicase and ... | |
| 1988 | T Z Su, M R el-Gewely | A multisite-directed mutagenesis usin... | |
| 1988 | S D Rabkin, Charles C Richardson | Initiation of DNA replication at clon... | |
| 1988 | T W Myers, Louis J Romano | Mechanism of stimulation of T7 DNA po... | |
| 1988 | H Nakai, Charles C Richardson | The effect of the T7 and Escherichia ... | |
| 1987 | H E Huber, Stanley Tabor, Charles C Richardson | Escherichia coli thioredoxin stabiliz... | |
| 1987 | Stanley Tabor, Charles C Richardson | Selective oxidation of the exonucleas... | |
| 1987 | Stanley Tabor, Charles C Richardson | DNA sequence analysis with a modified... | |
| 1987 | Stanley Tabor, H E Huber, Charles C Richardson | Escherichia coli thioredoxin confers ... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Processivity,Vmax,All properties |
| 1986 | H Nakai, Charles C Richardson | Interactions of the DNA polymerase an... | |
| 1986 | M L Michaels, M S Lee, Louis J Romano | Contrasting effects of Escherichia co... | |
| 1985 | C W Fuller, Charles C Richardson | Initiation of DNA replication at the ... | |
| 1985 | C W Fuller, Charles C Richardson | Initiation of DNA replication at the ... | |
| 1985 | Stanley Tabor, Charles C Richardson | A bacteriophage T7 RNA polymerase/pro... | |
| 1984 | I Slaby, B Lind, A Holmgren | T7 DNA polymerase is not a zinc-metal... | |
| 1983 | S Adler, P Modrich | T7-induced DNA polymerase. Requiremen... | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
| 1983 | P Bedinger, B M Alberts | The 3'-5' proofreading exonuclease of... | |
| 1983 | S W Matson, Charles C Richardson | DNA-dependent nucleoside 5'-triphosph... | |
| 1983 | M J Engler, Charles C Richardson | Bacteriophage T7 DNA replication. Syn... | |
| 1983 | R L Lechner, Charles C Richardson | A preformed, topologically stable rep... | |
| 1983 | M J Engler, R L Lechner, Charles C Richardson | Two forms of the DNA polymerase of ba... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Extension from RNA primer,Nick Extension,5-3' exo,Nucleotide Substitution Rate,Molecular Weight,Other accessory protein(s),All properties |
| 1983 | R L Lechner, M J Engler, Charles C Richardson | Characterization of strand displaceme... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Nick Extension,3-5' exo,Other accessory protein(s),All properties |
| 1983 | S W Matson, Stanley Tabor, Charles C Richardson | The gene 4 protein of bacteriophage T... | |
| 1982 | H Randahl, I Slabý, A Holmgren | An improved purification method and a... | |
| 1982 | H Randahl | Inhibition of the T7 DNA polymerase b... | |
| 1981 | Stanley Tabor, Charles C Richardson | Template recognition sequence for RNA... | |
| 1981 | A Holmgren, G B Kallis, B Nordström | A mutant thioredoxin from Escherichia... | |
| 1981 | Louis J Romano, F Tamanoi, Charles C Richardson | Initiation of DNA replication at the ... | |
| 1981 | B Nordström, H Randahl, I Slaby, A Holmgren | Characterization of bacteriophage T7 ... | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Molecular Weight,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
| 1979 | K Hori, D F Mark, Charles C Richardson | Deoxyribonucleic acid polymerase of b... | |
| 1979 | S Adler, P Modrich | T7-induced DNA polymerase. Characteri... | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
| 1979 | K Hori, D F Mark, Charles C Richardson | Deoxyribonucleic acid polymerase of b... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Isoelectric Point,3-5' exo,KM,3-5' Exo Specific Activity,Other accessory protein(s),All properties |
| 1979 | Louis J Romano, Charles C Richardson | Requirements for synthesis of ribonuc... | |
| 1978 | R Kolodner, Charles C Richardson | Gene 4 protein of bacteriophage T7. C... | |
| 1978 | R Kolodner, Y Masamune, J E LeClerc, Charles C Richardson | Gene 4 protein of bacteriophage T7. P... | |
| 1977 | R Kolodner, Charles C Richardson | Replication of duplex DNA by bacterio... | |
| 1976 | D F Mark, Charles C Richardson | Escherichia coli thioredoxin: a subun... | |
| 1975 | P Modrich, Charles C Richardson | Bacteriophage T7 deoxyribonucleic aci... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Molecular Weight,All properties |
| 1975 | P Modrich, Charles C Richardson | Bacteriophage T7 Deoxyribonucleic aci... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Other accessory protein(s),All properties |
| 1975 | David C Hinkle, Charles C Richardson | Bacteriophage T7 deoxyribonucleic aci... | |
| 1971 | Y Masamune, Charles C Richardson | Strand displacement during deoxyribon... | |
| 1971 | P Grippo, Charles C Richardson | Deoxyribonucleic acid polymerase of b... | Cloned or native,Tagged,Full length or truncated,3-5' exo,All properties |
| 1971 | Paul T Englund | The initial step of in vitro synthesi... |
Protein sequence:
| Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| T7 (exo-) | No (1) | ||||
| T7 E480D | |||||
| T7 Y526F | |||||
| T7 Y530F | |||||
| T7 K587N,K589N,R590N,R591N | |||||
| T7 (purified without EDTA) | Yes (2) | 4.5e-02 errors/bp (1) | |||
| Sequenase | No (1) | 3.4e-05-4.4e-05 errors/bp (2) | 4.4e-05-5.4e-05 errors/bp (2) | ||
| T7 (no thioreoxin) |
Protein information: Uniprot P00581 search for T7 at UniProt
Sequence information: NCBI nucleotides European nucleotide archive