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Results for property: kcat

Detailed Results for Property

Use this page to examine all the the polymerases and references referring to the property: k<sub>cat</sub>.

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Polymerase Kingdom Family Reference Result Context
T4 W213S Virus/Phage B Fidalgo da Silva E2007 1.5 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: Mg++ (8mM), Temp (37°C)
T4 Virus/Phage B Fidalgo da Silva E2007 145 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (with heparin); Experimental conditions: Temp (37°C), Mg++ (8mM)
T4 Virus/Phage B Fidalgo da Silva E2007 106 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Multiple Turnover (without heparin); Experimental conditions: Mg++ (8mM), Temp (37°C)
KF Y766S Eubacterium A Polesky AH1990 0.8 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench
Klenow fragment Eubacterium A Derbyshire V1991 0.09 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
Klenow fragment Eubacterium A Derbyshire V1991 0.12 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C)
Klenow fragment Eubacterium A Joyce CM2008 140 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Stopped flow (FRET assay of fingers-closing); Experimental conditions: pH (pH 7.5), Mg++ (5mM), Temp (22°C)
KF L361A Eubacterium ? Derbyshire V1991 0.044 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C)
KF L361A Eubacterium ? Derbyshire V1991 4E-05 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
KF L361M Eubacterium ? Derbyshire V1991 8.3E-05 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
KF D424A Eubacterium A Derbyshire V1991 1.3E-08 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Temp (37°C)
Terminal Transferase Unknown ? Boulé JB2001 76 /minute Reaction: Nucleotide incorporation; Substrate: rATP
Terminal Transferase Unknown ? Boulé JB2001 180 /minute Reaction: Nucleotide incorporation; Substrate: dATP
Terminal Transferase Unknown ? Boulé JB2001 147 /minute Reaction: Nucleotide incorporation; Substrate: dATP
Terminal Transferase Unknown ? Boulé JB2001 11.3 /minute Reaction: Nucleotide incorporation; Substrate: dATP
Bst LF Eubacterium A Kiefer JR1997 191.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding (16.5 nM primed M13); Experimental conditions: Temp (65°C)
Taq pol I Eubacterium A Kiefer JR1997 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding (16.5nM primed M13); Experimental conditions: Temp (72°C)
KF D424E Eubacterium A Derbyshire V1991 0.01 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C)
KF D424E Eubacterium A Derbyshire V1991 4E-05 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
KF D355A Eubacterium A Derbyshire V1991 8.3E-08 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA substrate, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
KF D424N Eubacterium A Derbyshire V1991 2.5E-08 /second Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
RB69 D114AE116A Virus/Phage B Hogg M2006 270 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
RB69 D114AE116A Virus/Phage B Hogg M2006 0.43 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 Virus/Phage B Elisseeva E1999 200 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA
T4 D324A Virus/Phage B Elisseeva E1999 0.01 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA (9.5)
T4 Y320FD324A Virus/Phage B Elisseeva E1999 0.1 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA
T4 D112AE114A Virus/Phage B Hogg M2006 361 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D112AE114A Virus/Phage B Hogg M2006 3.24 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D324A Virus/Phage B Hogg M2006 180 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D324A Virus/Phage B Hogg M2006 2.13 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
RB69 D327A Virus/Phage B Hogg M2006 224 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
RB69 D327A Virus/Phage B Hogg M2006 0.53 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Virus/Phage B Hogg M2006 402 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A Virus/Phage B Hogg M2006 3.36 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Virus/Phage B Hogg M2006 0.56 /second Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C)
T4 D219A Virus/Phage B Hogg M2006 0.18 /second Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T)
RB69 D222A Virus/Phage B Hogg M2006 320 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
RB69 D222A Virus/Phage B Hogg M2006 0.97 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
RB69 D222A Virus/Phage B Hogg M2006 0.43 /second Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C)
RB69 D222A Virus/Phage B Hogg M2006 0.04 /second Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T)
Klenow fragment Eubacterium A Lin TC1994 5.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Eubacterium A Lin TC1994 7.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Virus/Phage B Lin TC1994 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Virus/Phage B Lin TC1994 420 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Virus/Phage B Lin TC1994 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Virus/Phage B Lin TC1994 270 /second Reaction: 3-5' Exonuclease
T4 N388 Virus/Phage B Lin TC1994 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Virus/Phage B Frey MW1993 3 /second Reaction: Nucleotide incorporation; Substrate: dATP
T4 Virus/Phage B Abdus Sattar AK1996 65.1 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Virus/Phage B Abdus Sattar AK1996 0.16 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Virus/Phage B Abdus Sattar AK1996 0.0078 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Virus/Phage B Abdus Sattar AK1996 0.0037 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112N Virus/Phage B Abdus Sattar AK1996 0.001 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Virus/Phage B Abdus Sattar AK1996 2 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Virus/Phage B Abdus Sattar AK1996 0.08 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219AD324A Virus/Phage B Abdus Sattar AK1996 0.0034 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Virus/Phage B Abdus Sattar AK1996 0.096 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Virus/Phage B Abdus Sattar AK1996 6E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Virus/Phage B Abdus Sattar AK1996 0.0062 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Virus/Phage B Abdus Sattar AK1996 7E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
RB69 K302A Virus/Phage B Wang CX2004 0.0011 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
RB69 Virus/Phage B Zhang H2006 0.004 /second Reaction: Misincorporation; Substrate: dATP
RB69 Virus/Phage B Zhang H2006 0.011 /second Reaction: Misincorporation; Substrate: dGTP
RB69 Virus/Phage B Zhang H2006 0.77 /second Reaction: Misincorporation; Substrate: dCTP
RB69 Virus/Phage B Zhang H2006 0.041 /second Reaction: Misincorporation; Substrate: dGTP
RB69 Virus/Phage B Zhang H2006 0.001 /second Reaction: Misincorporation; Substrate: dCTP
RB69 Virus/Phage B Zhang H2006 0.09 /second Reaction: Misincorporation; Substrate: dTTP
RB69 K302A Virus/Phage B Wang CX2004 0.0033 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
RB69 K302A Virus/Phage B Wang CX2004 0.01 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
RB69 K302A Virus/Phage B Wang CX2004 0.032 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
RB69 K302A Virus/Phage B Wang CX2004 0.11 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
RB69 Virus/Phage B Zhang H2006 0.013 /second Reaction: Misincorporation; Substrate: dCTP
RB69 Virus/Phage B Zhang H2006 0.14 /second Reaction: Misincorporation; Substrate: dTTP
RB69 Virus/Phage B Zhang H2006 0.015 /second Reaction: Misincorporation; Substrate: dTTP
RB69 Virus/Phage B Zhang H2006 220 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 Virus/Phage B Zhang H2006 200 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 Virus/Phage B Zhang H2006 167 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 Virus/Phage B Zhang H2006 240 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 Virus/Phage B Zhang H2006 0.013 /second Reaction: Misincorporation; Substrate: dATP
RB69 Virus/Phage B Zhang H2006 0.003 /second Reaction: Misincorporation; Substrate: dGTP
RB69 Virus/Phage B Zhang H2006 0.08 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A Virus/Phage B Zhang H2006 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A Virus/Phage B Zhang H2006 0.11 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A Virus/Phage B Zhang H2006 0.26 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A Virus/Phage B Zhang H2006 0.18 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A Virus/Phage B Zhang H2006 0.03 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A Virus/Phage B Zhang H2006 0.8 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A Virus/Phage B Zhang H2006 0.14 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A Virus/Phage B Zhang H2006 0.05 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A Virus/Phage B Zhang H2006 0.13 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A Virus/Phage B Zhang H2006 108 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 L561A Virus/Phage B Zhang H2006 89 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 L561A Virus/Phage B Zhang H2006 101 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 L561A Virus/Phage B Zhang H2006 115 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 L561A Virus/Phage B Zhang H2006 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A Virus/Phage B Zhang H2006 0.01 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A Virus/Phage B Zhang H2006 0.75 /second Reaction: Misincorporation; Substrate: dATP
RB69 E116A Virus/Phage B Wang CX2004 3.9E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
RB69 E116A Virus/Phage B Wang CX2004 0.00011 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
RB69 E116A Virus/Phage B Wang CX2004 0.00028 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
RB69 E116A Virus/Phage B Wang CX2004 0.00075 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
RB69 E116A Virus/Phage B Wang CX2004 0.00053 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
RB69 Virus/Phage B Wang CX2004 17 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrat: 3'-tailed duplex phosphorothioate)
RB69 Virus/Phage B Wang CX2004 3 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
RB69 Virus/Phage B Wang CX2004 5 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
RB69 Virus/Phage B Wang CX2004 6.6 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
RB69 Virus/Phage B Wang CX2004 7.7 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
RB69 Virus/Phage B Wang CX2004 8.1 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
RB69 Virus/Phage B Wang CX2004 24 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 Virus/Phage B Wang CX2004 130 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 Virus/Phage B Wang CX2004 91 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 K229A Virus/Phage B Wang CX2004 0.18 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 K229A Virus/Phage B Wang CX2004 0.05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex phosphorothioate substrate)
RB69 Y323F Virus/Phage B Wang CX2004 0.14 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
RB69 Y323F Virus/Phage B Wang CX2004 0.00056 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
RB69 Y323F Virus/Phage B Wang CX2004 0.0018 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
RB69 Y323F Virus/Phage B Wang CX2004 0.0058 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
RB69 Y323F Virus/Phage B Wang CX2004 0.019 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
RB69 Y323F Virus/Phage B Wang CX2004 0.056 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
RB69 Y323F Virus/Phage B Wang CX2004 0.0013 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex phosphorothioate substrate)
T4 Y320F Virus/Phage B Wang CX2004 0.12 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 Y320F Virus/Phage B Wang CX2004 0.00096 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ( Substrate: 3'-tailed duplex phosphorothioate substrate)
T4 Y320A Virus/Phage B Wang CX2004 0.075 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
T4 Y320A Virus/Phage B Wang CX2004 0.0085 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex phosphorothioate substrate)
T4 K299AY320F Virus/Phage B Wang CX2004 0.013 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ( Substrate: 3'-tailed duplex phosphorothioate substrate)
T4 K299AY320F Virus/Phage B Wang CX2004 0.0005 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
RB69 K302AY323F Virus/Phage B Wang CX2004 0.013 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
RB69 K302AY323F Virus/Phage B Wang CX2004 0.00081 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex phosphorothioate substrate)
RB69 K302AY323F Virus/Phage B Wang CX2004 0.02 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex phosphorothioate substrate)
RB69 K302AY323F Virus/Phage B Wang CX2004 2.1E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
RB69 K302AY323F Virus/Phage B Wang CX2004 6.5E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
RB69 K302AY323F Virus/Phage B Wang CX2004 0.0002 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
RB69 K302AY323F Virus/Phage B Wang CX2004 0.00064 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
RB69 K302AY323F Virus/Phage B Wang CX2004 0.002 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
Klenow fragment Eubacterium A Wang CX2004 0.02 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ( Substrate: 3'-tailed duplex phosphorothioate substrate)
Klenow fragment Eubacterium A Wang CX2004 0.0017 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
Klenow fragment Eubacterium A Wang CX2004 0.0054 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
Klenow fragment Eubacterium A Wang CX2004 0.016 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
Klenow fragment Eubacterium A Wang CX2004 0.052 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
Klenow fragment Eubacterium A Wang CX2004 0.16 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
Klenow fragment Eubacterium A Wang CX2004 0.33 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
Dpo4 Archaeon Y Eoff RL2007 0.58 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Archaeon Y Eoff RL2007 0.055 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Archaeon Y Eoff RL2007 0.0066 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (O6-BzG)
Dpo4 Archaeon Y Eoff RL2007 0.0025 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (O6-BzG)
Dpo4 Archaeon Y Eoff RL2007 0.00083 /second Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (O6-BzG)
Dpo4 Archaeon Y Beckman JW2008 0.8 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template G)
Dpo4 N188W Archaeon Y Beckman JW2008 0.47 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State
Dpo4 T239W Archaeon Y Beckman JW2008 0.34 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State
HIV RT Virus/Phage RT Kopp EB1991 0.5 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State
HIV RT Virus/Phage RT Kopp EB1991 0.43 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State
Human Pol gamma Eukaryote A Graves SW1998 0.03 /second
Human Pol gamma Eukaryote A Graves SW1998 0.18 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
HIV RT Virus/Phage RT Kopp EB1991 0.12 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State
HIV RT Virus/Phage RT Kopp EB1991 0.77 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State
Human Pol delta Eukaryote B Einolf HJ2000 0.36 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (With PCNA)
Human Pol delta Eukaryote B Einolf HJ2000 0.3 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Without PCNA)
Bst LF Eubacterium A Kiefer JR1998 191.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Eubacterium A Kiefer JR1998 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Eubacterium A Kiefer JR1998 5.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol eta Eukaryote Y Washington MT2001 0.01 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
HIV RT Virus/Phage RT Reardon JE1990 0.025 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (RNA template)
HIV RT Virus/Phage RT Reardon JE1990 1.9 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench (RNA template)
HIV RT Virus/Phage RT Reardon JE1990 0.071 /second Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Rapid quench (RNA template)
GA-1 Virus/Phage B Longás E2006 2260 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Eubacterium A Minnick DT1996 0.3 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Vent A488L Archaeon B Gardner AF2004 0.47 /second Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Rapid quench (burst conditions); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 13 /second Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 0.63 /second Reaction: Pyrophosphorolysis; Substrate: DNA template; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 56 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 0.7 /second Reaction: Nucleotide incorporation; Substrate: rCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 0.3 /second Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 13 /second Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Vent A488L Archaeon B Gardner AF2004 45 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Rapid quench (burst conditions); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C)
Human Pol lamba Eukaryote X Bebenek K2010 0.012 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State
Human Pol lamba Eukaryote X Bebenek K2010 0.00014 /second Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State
FIV RT Virus/Phage RT Operario DJ2005 0.51 /second Reaction: Nucleotide incorporation; Substrate: dGTP
FIV RT Virus/Phage RT Operario DJ2005 0.21 /second Reaction: Nucleotide incorporation; Substrate: dATP
FIV RT Virus/Phage RT Operario DJ2005 0.28 /second Reaction: Nucleotide incorporation; Substrate: dTTP
FIV RT Virus/Phage RT Operario DJ2005 0.22 /second Reaction: Nucleotide incorporation; Substrate: dCTP
FeLV RT Virus/Phage RT Operario DJ2005 0.56 /second Reaction: Nucleotide incorporation; Substrate: dGTP
FeLV RT Virus/Phage RT Operario DJ2005 0.18 /second Reaction: Nucleotide incorporation; Substrate: dATP
FeLV RT Virus/Phage RT Operario DJ2005 0.17 /second Reaction: Nucleotide incorporation; Substrate: dTTP
FeLV RT Virus/Phage RT Operario DJ2005 0.15 /second Reaction: Nucleotide incorporation; Substrate: dCTP
Pfu Archaeon B Hogrefe HH2001 560 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Vent Archaeon B Hogrefe HH2001 1000 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Eubacterium A Hogrefe HH2001 2800 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Bst LF Eubacterium A Hogrefe HH2001 1.15E+04 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Eubacterium A Hogrefe HH2001 310 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce pol gamma Eukaryote A Viikov K2012 69 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Gel shift
Sce pol gamma ∆175 Eukaryote A Viikov K2012 74 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Gel shift
Sce pol gamma ∆216 Eukaryote A Viikov K2012 65 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Gel shift
All Questions

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