Dpo4

From S. solfataricus (aliases:dna polymerase 4, pol Y1)
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This page presents all the information in Polbase for Dpo4.

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Structures/Sequence:

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Dpo4 is a family Y enzyme from S. solfataricus.

Dpo4 was identified in 2001 (She et al. The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl Acad. Sci. USA, 2001; 98: 7835–7840) as a putative bypass polymerase (Sso2448) homologous to the Y family DinB SOS-induced polymerase IV of E. coli. This enzyme shares 53% sequence identity with the previously identified Dbh from closely related Sulfolobus acidocaldarius strain originally mistaken with Sulfolobus solfataricus P1 (Kulaeva et al. Identification of a DinB/UmuC homolog in the archeon Sulfolobus solfataricus. Mutat. Res., 1996; 357: 245–253). S. solfataricus P2 DinB has been termed DNA polymerase IV (Dpo4). It was first purified and characterized in 2001 (Boudsocq et al. Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic pol eta. Nucleic Acids Res., 2001; 29: 4607-16). In the same year, the first crystal structure of Dpo4 in ternary complexes with DNA and an incoming nucleotide, either correct or incorrect, has been published (Ling et al. Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Cell, 2001; 107: 9-12).

Selected Properties for Dpo4:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
No (1) No (1) 2.3e-03 errors/bp (1) 6.5e-03 errors/bp (1) 1.5e-04-3.2e-03 errors/bp (2)
Year Authors Title Results by property
2013 Ganesh Shanmugam, Irina G Minko, Surajit Banerjee, Plamen P Christov, Ivan D Kozekov, Carmelo J Rizzo, R Stephen Lloyd, Martin Egli, Michael P Stone Shanmugam G2013
2013 Shirly J Espinoza-Herrera, Vineet Gaur, Zucai Suo, Paul R Carey Espinoza-Herrera SJ2013
2013 Alessia Stornetta, Todor Angelov, F Peter Guengerich, Shana J Sturla Stornetta A2013
2012 Prakasha Gowda A S, Gowdahalli Krishnegowda, Zucai Suo, Shantu G Amin, Thomas E Spratt A S PG2012
2012 Yong Wang, Xiakun Chu, Zucai Suo, Erkang Wang, Jin Wang Wang Y2012
2012 Surajit Banerjee, Plamen P Christov, Albena Kozekova, Carmelo J Rizzo, Martin Egli, Michael P Stone Banerjee S2012
2012 Shanen M Sherrer, Brian A Maxwell, Lindsey R Pack, Kevin A Fiala, Jason D Fowler, Jun Zhang, Zucai Suo Sherrer SM2012
2012 Shanen M Sherrer, DJ Taggart, Lindsey R Pack, Chanchal K Malik, Ashis K Basu, Zucai Suo Sherrer SM2012
2011 Kevin N Kirouac, Zucai Suo, Hong Ling Kirouac KN2011
2011 Dejian Ma, Jason D Fowler, Zucai Suo Ma D2011
2011 Surajit Banerjee, Kyle L Brown, Martin Egli, Michael P Stone Banerjee S2011
2010 Shanen M Sherrer, David C Beyer, Cynthia X Xia, Jason D Fowler, Zucai Suo Sherrer SM2010
2010 Adriana Irimia, Lioudmila V Loukachevitch, Robert Lawton Eoff, F Peter Guengerich, Martin Egli Irimia A2010
2010 Dejian Ma, Jason D Fowler, Chunhua Yuan, Zucai Suo Ma D2010
2010 Olga Rechkoblit, Alexander Kolbanovskiy, Lucy Malinina, Nicholas E Geacintov, Suse Broyde, Dinshaw J Patel Rechkoblit O2010
2010 Likui Zhang, Huiqiang Lou, Li Guo, Zhengyan Zhan, Zhenhong Duan, Xin Guo, Li Huang Zhang L2010
2010 Jimson H Y Wong, Jessica A Brown, Zucai Suo, Paul Blum, Takehiko Nohmi, Hong Ling Wong JH2010
2009 Robert Lawton Eoff, Raymundo Sanchez-Ponce, F Peter Guengerich Eoff RL2009
2009 Haijiang Zhang, Robert Lawton Eoff, Ivan D Kozekov, Carmelo J Rizzo, Martin Egli, F Peter Guengerich Zhang H2009
2009 Guangxin Xing, Kevin N Kirouac, Yoon Jung Shin, Stephen D Bell, Hong Ling Xing G2009
2009 Shanen M Sherrer, Jessica A Brown, Lindsey R Pack, Vijay P Jasti, Jason D Fowler, Ashis K Basu, Zucai Suo Sherrer SM2009
2009 Sabine Schneider, Stephanie Schorr, Thomas Carell Schneider S2009
2009 Anna Valenti, Giuseppe Perugino, Takehiko Nohmi, Mosè Rossi, Maria Ciaramella Valenti A2009
2009 Cuiling Xu, Brian A Maxwell, Jessica A Brown, Likui Zhang, Zucai Suo Xu C2009
2009 Olga Rechkoblit, Lucy Malinina, Yuan Cheng, Nicholas E Geacintov, Suse Broyde, Dinshaw J Patel Rechkoblit O2009
2009 Haijiang Zhang, Robert Lawton Eoff, Ivan D Kozekov, Carmelo J Rizzo, Martin Egli, F Peter Guengerich Zhang H2009
2009 Haijiang Zhang, Robert Lawton Eoff, Ivan D Kozekov, Carmelo J Rizzo, Martin Egli, F Peter Guengerich Zhang H2009
2009 Robert Lawton Eoff, Jennifer B Stafford, Jozsef Szekely, Carmelo J Rizzo, Martin Egli, F Peter Guengerich, Lawrence J Marnett Eoff RL2009
2009 Robert Lawton Eoff, Jennifer B Stafford, Jozsef Szekely, Carmelo J Rizzo, Martin Egli, F Peter Guengerich, Lawrence J Marnett Eoff RL2009
2008 Jimson H Y Wong, Kevin A Fiala, Zucai Suo, Hong Ling Wong JH2008
2008 Suse Broyde, Liming Wang, Olga Rechkoblit, Nicholas E Geacintov, Dinshaw J Patel Broyde S2008
2008 Yazhen Wang, Sarah K Musser, Sam Saleh, Lawrence J Marnett, Martin Egli, Michael P Stone Wang Y2008
2008 Yazhen Wang, Tamar Schlick Wang Y2008
2008 Kevin A Fiala, Shanen M Sherrer, Jessica A Brown, Zucai Suo Fiala KA2008
2008 Lindsey DeCarlo, A S Prakasha Gowda, Zucai Suo, Thomas E Spratt DeCarlo L2008
2008 Jeff W Beckman, Qixin Wang, F Peter Guengerich Beckman JW2008 KM,Kd,kcat,All properties
2008 Jessica A Brown, Sean A Newmister, Kevin A Fiala, Zucai Suo Brown JA2008
2008 Patrick P Lestienne, François Boudsocq, Jacques E Bonnet Lestienne PP2008
2008 Pingna Xu, Lida Oum, Nicholas E Geacintov, Suse Broyde Xu P2008
2007 Kevin A Fiala, Zucai Suo Fiala KA2007
2007 Sushil Chandani, Edward L Loechler Chandani S2007
2007 Robert Lawton Eoff, Karen C Angel, Martin Egli, F Peter Guengerich Eoff RL2007 KM,kcat,All properties
2007 Flavia Barone, Scott D McCulloch, Peter Macpherson, Giovanni Maga, Masami Yamada, Takehiko Nohmi, Anna Minoprio, Filomena Mazzei, Thomas A Kunkel, Peter Karran, Margherita Bignami Barone F2007
2007 Robert Lawton Eoff, Adriana Irimia, Martin Egli, F Peter Guengerich Eoff RL2007
2007 Kevin A Fiala, Cameron D Hypes, Zucai Suo Fiala KA2007
2007 Adriana Irimia, Robert Lawton Eoff, Pradeep S Pallan, F Peter Guengerich, Martin Egli Irimia A2007
2007 Rebecca A Perlow-Poehnelt, Ilya Likhterov, Liming Wang, David A Scicchitano, Nicholas E Geacintov, Suse Broyde Perlow-Poehnelt RA2007 Template lesions,All properties
2007 Adam P Silverman, Qingfei Jiang, Myron F Goodman, Eric T Kool Silverman AP2007
2007 Liming Wang, Xinyun Yu, Po Hu, Suse Broyde, Yongqing Zhang Wang L2007
2007 Robert Lawton Eoff, Adriana Irimia, Karen C Angel, Martin Egli, F Peter Guengerich Eoff RL2007
2007 Adriana Irimia, Robert Lawton Eoff, Pradeep S Pallan, F Peter Guengerich, Martin Egli Irimia A2007
2007 Kevin A Fiala, Jessica A Brown, Hong Ling, Ajay K Kshetry, Jun Zhang, John-Stephen Taylor, Wei Wen Yang, Zucai Suo Fiala KA2007
2007 Jacob Bauer, Guangxin Xing, Haruhiko Yagi, Jane M Sayer, Donald M Jerina, Hong Ling Bauer J2007
2007 Wei Wen Yang, Roger Woodgate Yang WW2007
2006 Alexander K Showalter, Brandon J Lamarche, Marina Bakhtina, Mei-I Su, Kuo-Hsiang Tang, Ming-Daw Tsai Showalter AK2006
2006 F Peter Guengerich Guengerich FP2006
2006 Liming Wang, Suse Broyde Wang L2006
2006 Hong Zang, Goutam Chowdhury, Karen C Angel, Thomas M Harris, F Peter Guengerich Zang H2006
2006 Hong Zang, Adriana Irimia, Jeong-Yun Choi, Karen C Angel, Lioudmila V Loukachevitch, Martin Egli, F Peter Guengerich Zang H2006
2006 Shin Mizukami, Tae Woo Kim, Sandra A Helquist, Eric T Kool Mizukami S2006
2006 Ling Zhang, Olga Rechkoblit, Liming Wang, Dinshaw J Patel, Robert Shapiro, Suse Broyde Zhang L2006
2006 Olga Rechkoblit, Lucy Malinina, Yuan Cheng, Vitaly Kuryavyi, Suse Broyde, Nicholas E Geacintov, Dinshaw J Patel Rechkoblit O2006
2006 Takehiko Nohmi Nohmi T2006
2006 Chiu Hong Lee, Sushil Chandani, Edward L Loechler Lee CH2006
2006 James S Stover, Goutam Chowdhury, Hong Zang, F Peter Guengerich, Carmelo J Rizzo Stover JS2006
2006 John P McDonald, Ashley Hall, Didier Gasparutto, Jean Cadet, Jack Ballantyne, Roger Woodgate McDonald JP2006
2006 Yazhen Wang, Karunesh Arora, Tamar Schlick Wang Y2006
2006 Adriana Irimia, Hong Zang, Lioudmila V Loukachevitch, Robert Lawton Eoff, F Peter Guengerich, Martin Egli Irimia A2006
2005 Satya Prakash, Robert E Johnson, Louise Prakash Prakash S2005
2005 Robert E Johnson, Louise Prakash, Satya Prakash Johnson RE2005
2005 Hong Zang, Angela K Goodenough, Jeong-Yun Choi, Adriana Irimia, Lioudmila V Loukachevitch, Ivan D Kozekov, Karen C Angel, Carmelo J Rizzo, Martin Egli, F Peter Guengerich Zang H2005
2005 Liming Wang, Min Wu, S Frank Yan, Dinshaw J Patel, Nicholas E Geacintov, Suse Broyde Wang L2005
2005 Angela K Goodenough, Ivan D Kozekov, Hong Zang, Jeong-Yun Choi, F Peter Guengerich, Thomas M Harris, Carmelo J Rizzo Goodenough AK2005
2005 Paula L Fischhaber, Errol C Friedberg Fischhaber PL2005
2005 Wei Wen Yang Yang WW2005
2005 Paul J Rothwell, Gabriel Waksman Rothwell PJ2005
2005 Alexandra Vaisman, Hong Ling, Roger Woodgate, Wei Wen Yang Vaisman A2005
2005 Hong Zang, Angela K Goodenough, Jeong-Yun Choi, Adriana Irimia, Lioudmila V Loukachevitch, Ivan D Kozekov, Karen C Angel, Carmelo J Rizzo, Martin Egli, F Peter Guengerich Zang H2005
2005 Janina Cramer, Tobias Restle Cramer J2005
2005 Matthew Hogg, Susan S Wallace, Sylvie Doublié Hogg M2005
2004 Thomas A Kunkel Kunkel TA2004
2004 Brian S Plosky, Roger Woodgate Plosky BS2004
2004 Brigette Tippin, Phuong Pham, Myron F Goodman Tippin B2004
2004 Rebecca A Perlow-Poehnelt, Ilya Likhterov, David A Scicchitano, Nicholas E Geacintov, Suse Broyde Perlow-Poehnelt RA2004
2004 Catherine M Joyce, Stephen J Benkovic Joyce CM2004
2004 Katarzyna Bebenek, Thomas A Kunkel Bebenek K2004
2004 Hong Ling, François Boudsocq, Roger Woodgate, Wei Wen Yang Ling H2004
2004 Hong Ling, Jane M Sayer, Brian S Plosky, Haruhiko Yagi, François Boudsocq, Roger Woodgate, Donald M Jerina, Wei Wen Yang Ling H2004
2004 Oliver Fleck, Primo Schär Fleck O2004
2004 Kevin A Fiala, Zucai Suo Fiala KA2004
2004 Kevin A Fiala, Zucai Suo Fiala KA2004 Cloned or native,Tagged,Tag Name,Full length or truncated,Vmax,Overall Error Rate,All properties
2004 François Boudsocq, Robert J Kokoska, Brian S Plosky, Alexandra Vaisman, Hong Ling, Thomas A Kunkel, Wei Wen Yang, Roger Woodgate Boudsocq F2004
2004 José Trincao, Robert E Johnson, William T Wolfle, Carlos R Escalante, Satya Prakash, Louise Prakash, Aneel K Aggarwal Trincao J2004
2003 Wei Wen Yang Yang WW2003
2003 Miguel García-Díaz, José F Ruiz, Raquel Juárez, Gloria Terrados, Luis Blanco García-Díaz M2003
2003 Hong Ling, François Boudsocq, Brian S Plosky, Roger Woodgate, Wei Wen Yang Ling H2003
2003 Suzanne Sommer, Olivier J Becherel, Geneviève Coste, Adriana Bailone, Robert P P Fuchs Sommer S2003
2003 Robert J Kokoska, Scott D McCulloch, Thomas A Kunkel Kokoska RJ2003
2003 William A Beard, Samuel H Wilson Beard WA2003
2002 John-Stephen Taylor Taylor JS2002
2002 Robert J Kokoska, Katarzyna Bebenek, François Boudsocq, Roger Woodgate, Thomas A Kunkel Kokoska RJ2002 3-5' exo,5-3' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties
2002 François Boudsocq, Hong Ling, Wei Wen Yang, Roger Woodgate Boudsocq F2002
2001 Petr Grúz, Francesca M Pisani, Masatomi Shimizu, Masami Yamada, I Hayashi, Kosuke Morikawa, Takehiko Nohmi Grúz P2001
2001 Errol C Friedberg, Paula L Fischhaber, C Kisker Friedberg EC2001
2001 François Boudsocq, Shigenori Iwai, Fumio Hanaoka, Roger Woodgate Boudsocq F2001 Cloned or native,Tagged,Full length or truncated,Isoelectric Point,Molecular Weight,Template lesions,All properties
2001 Hong Ling, François Boudsocq, Roger Woodgate, Wei Wen Yang Ling H2001 Cloned or native,Full length or truncated,Amino Acids Contacting Template,Amino Acids Contacting NTP,Polymerase Catalytic Residue Amino Acids,All properties
2001 B L Zhou, Janice D Pata, Thomas A Steitz Zhou BL2001
1998 Francesca M Pisani, Mariarita De Felice, G Manco, Mosè Rossi Pisani FM1998
Protein sequence:
DNA sequence:
Name 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Dpo4 y12A
Dpo4 N188W
Dpo4 T239W
Dpo4 x331AR332A
Dpo4 R332L
Dpo4 R332A
Dpo4 R332E
Dpo4 R332H
All Polymerases | 37 Results

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