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Polymerase: HIV RT

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Polymerase Reference Property Result Context
HIV RT Graves SW1998 Processivity 200bp
HIV RT Graves SW1998 Vmax 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
HIV RT Graves SW1998 Kd 4.7nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
HIV RT Graves SW1998 Kd 14uM Reaction: Nucleotide incorporation; Substrate: dNTPs
HIV RT Arion D1996 Reverse Transcriptase Activity Yes
HIV RT Arion D1996 Cloned or native Cloned in E. coli
HIV RT Arion D1996 Full length or truncated Full length
HIV RT Arion D1996 Extension from RNA primer Yes
HIV RT Huber HE1989 3-5' Exonuclease (proofreading) No
HIV RT Huber HE1989 5-3' Exonuclease No
HIV RT Huber HE1989 RNase H Yes
HIV RT Huber HE1989 Processivity 15bp
HIV RT Huber HE1989 KM 10uM Reaction: Nucleotide incorporation; Substrate: dTTP
HIV RT Huber HE1989 Kd 2.3uM Reaction: Polymerase-DNA interaction; Substrate: RNA-DNA hybrid
HIV RT DeStefano JJ1992 Reverse Transcriptase Activity Yes
HIV RT DeStefano JJ1992 Molecular Weight 1.17E+05 Dalton
HIV RT DeStefano JJ1992 Full length or truncated Full length
HIV RT DeStefano JJ1992 RNase H Yes
HIV RT DeStefano JJ1992 Processivity 75bp
HIV RT DeStefano JJ1992 Specific Activity 2.1E+04 units/mg
HIV RT Kopp EB1991 KM 10nM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State
HIV RT Kopp EB1991 KM 40nM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State
HIV RT Kopp EB1991 KM 20nM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State
HIV RT Kopp EB1991 KM 100nM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State
HIV RT Kopp EB1991 kcat 0.5 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State
HIV RT Kopp EB1991 kcat 0.43 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State
HIV RT Kopp EB1991 kcat 0.12 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State
HIV RT Kopp EB1991 kcat 0.77 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State
HIV RT Reardon JE1990 KM 0.082uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (RNA template)
HIV RT Reardon JE1990 KM 10uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench (RNA template)
HIV RT Reardon JE1990 KM 0.19uM Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Rapid quench
HIV RT Reardon JE1990 kcat 0.025 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (RNA template)
HIV RT Reardon JE1990 kcat 1.9 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench (RNA template)
HIV RT Reardon JE1990 kcat 0.071 /second Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Rapid quench (RNA template)
HIV RT Kertesz DJ2010 Reverse Transcriptase Activity Yes
HIV RT Mitchell ML2010 Reverse Transcriptase Activity Yes
HIV RT Mitchell ML2010 Reverse Transcriptase Activity Yes
HIV RT Kellinger MW2010 Reverse Transcriptase Activity Yes
HIV RT Su HP2010 Reverse Transcriptase Activity Yes
HIV RT Su HP2010 RNase H Yes
HIV RT Freisz S2010 Reverse Transcriptase Activity Yes
HIV RT Cullen MD2009 Reverse Transcriptase Activity Yes
HIV RT Su DS2009 Reverse Transcriptase Activity Yes
HIV RT Himmel DM2009 Reverse Transcriptase Activity Yes
HIV RT Himmel DM2009 RNase H Yes
HIV RT Himmel DM2006 Reverse Transcriptase Activity Yes
HIV RT Himmel DM2006 RNase H Yes
HIV RT Gopalakrishnan V1992 Reverse Transcriptase Activity Yes
HIV RT Gopalakrishnan V1992 Terminal Transferase Unspecified
HIV RT Gopalakrishnan V1992 RNase H Yes
HIV RT Furfine ES1991 Reverse Transcriptase Activity Yes
HIV RT Hafkemeyer P1991 Reverse Transcriptase Activity Yes
HIV RT Hafkemeyer P1991 RNase H Yes
HIV RT Corbau R2010 Reverse Transcriptase Activity Yes
HIV RT Das K2009 Reverse Transcriptase Activity Yes
HIV RT Sweeney ZK2008 Reverse Transcriptase Activity Yes
HIV RT Kirschberg TA2008 Reverse Transcriptase Activity Yes
HIV RT Sweeney ZK2008 Reverse Transcriptase Activity Yes
HIV RT Lisco A2008 Reverse Transcriptase Activity Yes
HIV RT Tucker TJ2008 Reverse Transcriptase Activity Yes
HIV RT Ren J2008 Reverse Transcriptase Activity Yes
HIV RT Tucker TJ2008 Reverse Transcriptase Activity Yes
HIV RT Das K2008 Reverse Transcriptase Activity Yes
HIV RT Spallarossa A2008 Reverse Transcriptase Activity Yes
HIV RT Zhao Z2008 Reverse Transcriptase Activity Yes
HIV RT Bauman JD2008 Reverse Transcriptase Activity Yes
HIV RT Dharmasena S2007 Reverse Transcriptase Activity Yes
HIV RT Marchand B2007 Reverse Transcriptase Activity Yes
HIV RT El Safadi Y2007 Reverse Transcriptase Activity Yes
HIV RT Ren J2007 Reverse Transcriptase Activity Yes
HIV RT Ohrui H2006 Reverse Transcriptase Activity Yes
HIV RT Jochmans D2006 Reverse Transcriptase Activity Yes
HIV RT Götte M2006 Reverse Transcriptase Activity Yes
HIV RT Himmel DM2005 Reverse Transcriptase Activity Yes
HIV RT Himmel DM2005 Associated condition HIV
HIV RT Andries K2004 Reverse Transcriptase Activity Yes
HIV RT Andries K2004 Associated condition HIV
HIV RT Rezende LF2004 Reverse Transcriptase Activity Yes
HIV RT Rezende LF2004 Associated condition HIV
HIV RT Painter GR2004 Reverse Transcriptase Activity Yes
HIV RT Painter GR2004 Associated condition HIV
HIV RT Peletskaya EN2004 Reverse Transcriptase Activity Yes
HIV RT Peletskaya EN2004 Associated condition HIV
HIV RT Freeman GA2004 Reverse Transcriptase Activity Yes
HIV RT Freeman GA2004 Associated condition HIV
HIV RT Gallant JE2003 Reverse Transcriptase Activity Yes
HIV RT Gallant JE2003 Associated condition HIV
HIV RT Ray AS2003 Reverse Transcriptase Activity Yes
HIV RT Ray AS2003 Associated condition HIV
HIV RT Anderson KS2002 Reverse Transcriptase Activity Yes
HIV RT Anderson KS2002 Associated condition HIV
HIV RT Fisher TS2002 Reverse Transcriptase Activity Yes
HIV RT Fisher TS2002 Associated condition HIV
HIV RT Cramer J2002 Reverse Transcriptase Activity Yes
HIV RT Cramer J2002 Associated condition HIV
HIV RT Li W2010 Reverse Transcriptase Activity Yes
HIV RT Operario DJ2005 Molecular Weight 66kD
HIV RT Operario DJ2005 Overall Error Rate 0.0005 errors/bp
HIV RT DeVico A1991 Reverse Transcriptase Activity Yes
HIV RT DeVico A1991 3-5' Exonuclease (proofreading) No
HIV RT DeVico A1991 Nucleotide Substitution Rate 0.003155 errors/bp
HIV RT DeVico A1991 Nucleotide Substitution Rate 0.001477 errors/bp
HIV RT DeVico A1991 Full length or truncated Full length
HIV RT DeVico A1991 RNase H Yes
HIV RT Boyer PL2007 Frameshift Error Rate 8E-06 errors/bp
HIV RT Boyer PL2007 Overall Error Rate 7.5E-05 errors/bp
HIV RT Boyer PL2007 Nucleotide Substitution Rate 6.3E-05 errors/bp
HIV RT Boyer PL2007 Full length or truncated Full length
HIV RT Eckert KA1993 3-5' Exonuclease (proofreading) No
HIV RT Eckert KA1993 Cloned or native Cloned in E. coli
HIV RT Eckert KA1993 Nucleotide Substitution Rate 2.63E-05 errors/bp Technique: Reversion (pH 6.5)
HIV RT Eckert KA1993 Nucleotide Substitution Rate 0.00027 errors/bp Technique: Reversion (pH 8.0)
HIV RT Eckert KA1993 Full length or truncated Full length
HIV RT Eckert KA1993 Specific Activity 1 umol/min/mg
HIV RT Menéndez-Arias L2009 Frameshift Error Rate 0.00023 errors/bp
HIV RT Bakhanashvili M1992 Reverse Transcriptase Activity Yes
HIV RT Bakhanashvili M1992 3-5' Exonuclease (proofreading) No
HIV RT Bebenek K1995 Frameshift Error Rate 0.0047 errors/bp Technique: Reversion
HIV RT Bebenek K1995 Overall Error Rate 0.08 errors/bp Technique: M13mp2 forward mutation assay
HIV RT Bebenek K1995 Overall Error Rate 0.0001 errors/bp Technique: M13mp2 opal codon reversion assay
HIV RT Lansdon EB2010 Reverse Transcriptase Activity No
HIV RT Lansdon EB2010 Associated condition HIV
HIV RT Lansdon EB2010 Reverse Transcriptase Activity Yes
HIV RT Lansdon EB2010 Polymerase Catalytic Residue Amino Acids D110, D185, D186
HIV RT Lansdon EB2010 Incorporation of non-standard nucleotides Unspecified
HIV RT Lansdon EB2010 Cloned or native Cloned in E. coli
HIV RT Lansdon EB2010 Tagged Yes
HIV RT Lansdon EB2010 Tag Name his
HIV RT Lansdon EB2010 Residues Involved in Catalysis of RNase H D443, E478, D498, D549
HIV RT Lansdon EB2010 Full length or truncated Full length
HIV RT Yap SH2007 Reverse Transcriptase Activity Yes
HIV RT Yap SH2007 Cloned or native Cloned in E. coli
HIV RT Yap SH2007 Tagged Yes
HIV RT Yap SH2007 Tag Name histidine
HIV RT Yap SH2007 Full length or truncated Full length
HIV RT Yap SH2007 RNase H Yes
HIV RT Yap SH2007 Storage requirements 50% glycerol -20oC
HIV RT Hopkins AL2004 Reverse Transcriptase Activity Yes
HIV RT Hopkins AL2004 Associated condition HIV

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.