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Results for property: Vmax

Detailed Results for Property

Use this page to examine all the the polymerases and references referring to the property: V<sub>max</sub>.

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Polymerase Kingdom Family Reference Result Context
T7 Virus/Phage A Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Virus/Phage A Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. 300 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Virus/Phage A Thermostable DNA polymerases. 330 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Virus/Phage A Thermostable DNA polymerases. 700 /second Reaction: 3-5' Exonuclease
T7 Virus/Phage A Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 105 /second Reaction: 3-5' Exonuclease
T7 Virus/Phage A Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 287 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Virus/Phage A Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
KOD1 Archaeon B Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. 138 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Vent Archaeon B Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. 1000 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Virus/Phage B Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. 0 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Virus/Phage B Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. 230 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Virus/Phage B Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. 55 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Virus/Phage B Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. 125 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 27 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 Virus/Phage B Processivity of the gene 41 DNA helicase at the bacteriophage T4 DNA replication fork. 400 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Virus/Phage B Efficient in vitro replication of double-stranded DNA templates by a purified T4 bacteriophage replication system. 500 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 54 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 91 /minute Reaction: 3-5' Exonuclease
T4 Virus/Phage B Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 2.2 /minute Reaction: 3-5' Exonuclease; Technique: Steady State (substrate: E. coli DNA, heat denatured)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 50 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 160 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 80 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 1120 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 800 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 60 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B The dynamic processivity of the T4 DNA polymerase during replication. 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 1100 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 350 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 850 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 Virus/Phage B T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. 60 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 0.33 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 0.66 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 0.15 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 A737V Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 0.52 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 1.72 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V Virus/Phage B An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. 0.86 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.04 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.0003 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.03 /second Substrate: dCTP
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.04 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.01 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.004 /second Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.01 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Virus/Phage B Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. 0.005 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Eubacterium A Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. 1.32 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Eubacterium A Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. 0.11 /second Reaction: Nucleotide incorporation; Substrate: dCTP
Eco Pol I Eubacterium A Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. 2.5 /second Reaction: Nucleotide incorporation; Substrate: dATP
Eco Pol I Eubacterium A Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. 3.8 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Eco Pol I Eubacterium A Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. 3.4 /second Reaction: Nucleotide incorporation; Substrate: dGTP
Klenow fragment Eubacterium A Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. 5.4 /minute Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Eubacterium A Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. 14 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Klenow fragment Eubacterium A Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. 23 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Klenow fragment Eubacterium A Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. 8.4 /minute Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCoTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 19 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
Klenow fragment Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 4.8 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
Klenow fragment Eubacterium A Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. 8.4 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State ; Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Eubacterium A Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. 50 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Dpo4 Archaeon Y Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. 3.8 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol eta Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 4 /minute Reaction: Nucleotide incorporation; Substrate: dATP
Human Pol eta Eukaryote Y Yeast DNA polymerase eta utilizes an induced-fit mechanism of nucleotide incorporation. 1.3 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol eta Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 2.1 /minute Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol eta Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 2.2 /minute Reaction: Nucleotide incorporation; Substrate: dCTP
Human Pol eta Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 3.6 /minute Reaction: Nucleotide incorporation; Substrate: dGTP
Human Pol gamma Eukaryote A Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. 3.5 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Eco Pol IV Eubacterium Y The beta clamp targets DNA polymerase IV to DNA and strongly increases its processivity. 0.3 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol beta Eukaryote X Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 27 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Standing start primer template)
Human Pol beta Eukaryote X Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 18 /second Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Standing start primer template)
Human Pol beta Eukaryote X Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 1.5 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Standing start primer template)
T5 Virus/Phage A Exonuclease associated with bacteriophage T5-Induced DNA polymerase. 10 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template
Taq pol I Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (75°C)
Taq pol I Eubacterium A Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
HIV RT Virus/Phage RT Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu Archaeon B Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. 25 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu Archaeon B Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis 21 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Tth Pol III Eubacterium C DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. 350 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (72°C)
Human Pol lamba Eukaryote X DNA polymerase family X: function, structure, and cellular roles. 2.1 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol mu Eukaryote X DNA polymerase family X: function, structure, and cellular roles. 0.076 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Phi29 Virus/Phage B Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase. 500 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
Phi29 Virus/Phage B Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. 2280 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Phi29 Virus/Phage B TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. 50 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
PGBD Pol I Archaeon B Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. 23 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 A89TD363N Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 11 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A89TD363N Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. 790 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 Q731am Virus/Phage B Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 1.1 /minute Reaction: 3-5' Exonuclease
T4 Q731am Virus/Phage B Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 83 /minute Reaction: 3-5' Exonuclease
Thermo JDF-3 pol Archaeon B Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. 2500 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol alpha Eukaryote B Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 0.06 /second Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Running start primer template)
Human Pol alpha Eukaryote B Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 2 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Running start primer template)
Human Pol alpha Eukaryote B Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. 1.7 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Eukaryote B Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. 1.1 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Eukaryote B Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. 1.7 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Running start primer template)
Human Pol alpha Eukaryote B DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. 13 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Gel shift
Human Pol alpha Eukaryote B DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Gel shift
Human Pol alpha Eukaryote B DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. 12.9 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Gel shift
Human Pol alpha Eukaryote B DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. 3.1 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Gel shift
Human Pol alpha Eukaryote B DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Gel shift
Eco Pol II Eubacterium B Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins. 25 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce Pol delta Eukaryote B Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta. 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Pol eta Y52E Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 0.5 /minute Reaction: Nucleotide incorporation; Substrate: dCTP
Pol eta Y52E Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 2.1 /minute Reaction: Nucleotide incorporation; Substrate: dTTP
Pol eta Y52E Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 3.5 /minute Reaction: Nucleotide incorporation; Substrate: dGTP
Pol eta Y52E Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. 1.3 /minute Reaction: Nucleotide incorporation; Substrate: dATP
Tgu Archaeon B Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 (exo-) Virus/Phage A Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 1 /second Reaction: Pyrophosphorolysis
T7 (exo-) Virus/Phage A Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 0.00069 /second Reaction: 3-5' Exonuclease
T7 (exo-) Virus/Phage A Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 242 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Vaccinia Virus Pol Virus/Phage B Characterization of a processive form of the vaccinia virus DNA polymerase. 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Stoffel fragment Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 130 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (75°C)
SsoDpo1 Archaeon B A trimeric DNA polymerase complex increases the native replication processivity. 422 /second
Nf Virus/Phage B Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. 2400 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce Pol delta* Eukaryote B Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta. 100 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs

Version:20240415

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