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Results for property: Molecular Weight

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Use this page to examine all the the polymerases and references referring to the property: Molecular Weight.

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Polymerase Kingdom Family Reference Result Context
T7 Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. 9.6E+04 Dalton
T7 Virus/Phage A Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. 9.6E+04 Dalton Technique: SDS-PAGE (2 subunits: 84 kD gene 5 protein + 12 kD TsnC protein)
T7 Virus/Phage A Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. 112kD
T7 Virus/Phage A T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. 9.2E+04 Dalton
9egN Archaeon B Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. 90kD Technique: SDS-PAGE (>95% pure)
Vent Archaeon B Intervening sequences in an Archaea DNA polymerase gene. 93kD Technique: SDS-PAGE
T4 Virus/Phage B On the exonuclease activity of phage T4 deoxyribonucleic acid polymerase. 1.15E+05 Dalton
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. 1.12E+05 Dalton
Eco Pol I Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. 1.09E+05 Dalton Technique: Sedimentation Equilibrium
Eco Pol I Eubacterium A Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins. 1.03E+05 Dalton
Eco Pol I Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 1.09E+05 Dalton
Eco Pol I Eubacterium A Thermostable DNA polymerases. 1.03E+05 Dalton
Eco Pol I Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 1.09E+05 Dalton
Klenow fragment Eubacterium A A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. 6.8E+04 Dalton
Klenow fragment Eubacterium A Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. 6.8E+04 Dalton
Klenow fragment Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 7.6E+04 Dalton Technique: SDS-acrylamide gel electrophoresis
Klenow fragment Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 7.6E+04 Dalton
Klenow fragment Eubacterium A Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. 7E+04 Dalton Technique: Gel Filtration
Dpo4 Archaeon Y Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. 4.019E+04 Dalton
Human Pol gamma Eukaryote A The mitochondrial p55 accessory subunit of human DNA polymerase gamma enhances DNA binding, promotes processive DNA synthesis, and confers N-ethylmaleimide resistance. 1.4E+05 Dalton
Human Pol gamma Eukaryote A The fidelity of human DNA polymerase gamma with and without exonucleolytic proofreading and the p55 accessory subunit. 140kD
Human Pol gamma Eukaryote A Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. 1.38E+05 Dalton
Human Pol gamma Eukaryote A Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity. 1.36E+05 Dalton
Human Pol gamma Eukaryote A Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity. 1.36E+05 Dalton
S pombe pol gamma Eukaryote A Characterization of a new DNA polymerase from Schizosaccharomyces pombe: a probable homologue of the Saccharomyces cerevisiae DNA polymerase gamma. 116kD Technique: Sequence
Eco Pol IV Eubacterium Y The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis. 3.2E+04 Dalton Technique: Gel Filtration
Eco Pol IV Eubacterium Y The beta clamp targets DNA polymerase IV to DNA and strongly increases its processivity. 3.95E+04 Dalton
Eco Pol V Eubacterium Y Purification and characterization of an inducible Escherichia coli DNA polymerase capable of insertion and bypass at abasic lesions in DNA. 8.4E+04 Dalton
Human Pol beta Eukaryote X Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme. 3.9E+04 Dalton Technique: SDS-PAGE
Human Pol beta Eukaryote X Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism. 39kD
Human Pol beta Eukaryote X DNA polymerase family X: function, structure, and cellular roles. 39kD
Human Pol beta Eukaryote X Thermostable DNA polymerases. 1.25E+05 Dalton
Streptococcus pneumoniae Pol I Eubacterium A Characterization of the polA gene of Streptococcus pneumoniae and comparison of the DNA polymerase I it encodes to homologous enzymes from Escherichia coli and phage T7. 9.949E+04 Dalton Technique: Sequence
Taq pol I Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 9.4E+04 Dalton
Taq pol I Eubacterium A Thermostable DNA polymerases. 9.39E+04 Dalton Technique: Sequence
Tma Pol III Eubacterium C Thermostable DNA polymerases. 103kD
HIV RT Virus/Phage RT Comparison of DNA polymerase activities between recombinant feline immunodeficiency and leukemia virus reverse transcriptases. 66kD
HIV RT Virus/Phage RT Parameters that influence processive synthesis and site-specific termination by human immunodeficiency virus reverse transcriptase on RNA and DNA templates. 1.17E+05 Dalton
Eco Pol III Eubacterium C Purification and characterization of DNA polymerase III'. Identification of tau as a subunit of the DNA polymerase III holoenzyme. 1.6E+05 Dalton
Eco Pol III Eubacterium C Thermostable DNA polymerases. 1.3E+05 Dalton
Eco Pol III Eubacterium C DNA polymerase III holoenzyme of Escherichia coli. Purification and resolution into subunits. 1.4E+05 Dalton
Tth Pol III Eubacterium C DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. 1.35E+05 Dalton Technique: SDS-PAGE
Human Pol lamba Eukaryote X DNA polymerase family X: function, structure, and cellular roles. 68kD
Human Pol lamba Eukaryote X DNA polymerase lambda, a novel DNA repair enzyme in human cells. 6.8E+04 Dalton Technique: SDS-PAGE
Human Pol lamba Eukaryote X Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. 68kD Technique: Gel Filtration
Human Pol mu Eukaryote X DNA polymerase family X: function, structure, and cellular roles. 55kD
Human Pol mu Eukaryote X DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells. 5.5E+04 Dalton Technique: SDS-PAGE
Human Pol mu Eukaryote X Highly frequent frameshift DNA synthesis by human DNA polymerase mu. 60 Dalton Technique: SDS-PAGE
Phi29 Virus/Phage B Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication. 6.6E+04 Dalton
Phi29 Virus/Phage B Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication. 6.8E+04 Dalton Technique: SDS-PAGE
Phi29 Virus/Phage B The bacteriophage phi 29 DNA polymerase, a proofreading enzyme. 6.652E+04 Dalton
Bst Eubacterium A Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity. 99.01kD Technique: Sequence
Bst Eubacterium A Thermostable DNA polymerases. 95kD
MMuLV RT Virus/Phage RT Studies on reverse transcriptase of RNA tumor viruses III. Properties of purified Moloney murine leukemia virus DNA polymerase and associated RNase H. 8E+04 Dalton
MMuLV RT Virus/Phage RT Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro. 84 Dalton
MMuLV RT Virus/Phage RT Comparison of DNA polymerase activities between recombinant feline immunodeficiency and leukemia virus reverse transcriptases. 71kD
Bst LF Eubacterium A Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity. 67kD Technique: SDS-PAGE (Cleaved from MBP tag)
Bsu Eubacterium ? DNA polymerase III gene of Bacillus subtilis. 1.513E+05 Dalton Technique: Sequence
AMV Virus/Phage RT Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro. 158kD
AMV Virus/Phage RT RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase. 1.73E+05 Dalton
pB6B15.23 Unknown ? Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. 7.1E+04 Dalton Technique: SDS-PAGE
MSV Virus/Phage RT Purification and characterization of the DNA polymerase and RNase H activities in Moloney murine sarcoma-leukemia virus. 7E+04 Dalton
Terminal Transferase Unknown ? Molecular biology of terminal transferase. 5.8E+04 Dalton
T4 Q731am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 90kD
T7 (purified without EDTA) Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. 9.6E+04 Dalton
T4 W844am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 100kD Technique: SDS-PAGE
Eco Pol II Eubacterium B Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins. 8.99E+04 Dalton
Eco Pol II Eubacterium B Thermostable DNA polymerases. 9E+04 Dalton
EBV Pol Virus/Phage B Characterization of an Epstein-Barr virus-induced DNA polymerase. 1.5E+05 Dalton
Human Pol delta Eukaryote B Thermostable DNA polymerases. 1.25E+05 Dalton
Human Pol delta Eukaryote B Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. 173kD
Human Pol epsilon Eukaryote B Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. 2.15E+05 Dalton Technique: SDS-PAGE
T4 Q730am Virus/Phage ? On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 90kD Technique: SDS-PAGE
T4 W593am Virus/Phage ? On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 76kD Technique: SDS-PAGE
T4 W458am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 62kD Technique: SDS-PAGE
T4 Q430am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 58kD Technique: SDS-PAGE
T4 Q386am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 54kD Technique: SDS-PAGE
T4 W202am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 26kD Technique: SDS-PAGE
T4 W213am Virus/Phage B On the direction of translation of the T4 deoxyribonucleic acid polymerase gene in vivo. 26kD Technique: SDS-PAGE
Sce Pol alpha Eukaryote B Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. 180kD
Sce Pol alpha Eukaryote B Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 180kD
Sce Pol delta Eukaryote B Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta. 520kD
HIV2 RT Virus/Phage RT Cloning, expression, purification, and crystallisation of HIV-2 reverse transcriptase. 40kD
Human Pol theta Eukaryote A Low-fidelity DNA synthesis by human DNA polymerase theta. 290kD
Tae pol A Eukaryote B Mammalian proliferating cell nuclear antigen stimulates the processivity of two wheat embryo DNA polymerases. 1.5E+05 Dalton
Tae pol B Eukaryote ? Mammalian proliferating cell nuclear antigen stimulates the processivity of two wheat embryo DNA polymerases. 1.2E+05 Dalton
Tae pol CI Eukaryote ? Mammalian proliferating cell nuclear antigen stimulates the processivity of two wheat embryo DNA polymerases. 6E+04 Dalton
Tae pol CII Eubacterium ? Mammalian proliferating cell nuclear antigen stimulates the processivity of two wheat embryo DNA polymerases. 1.5E+05 Dalton
Tgu Archaeon B Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis 9.026E+04 Dalton Technique: Sequence
Eco Pol III' Eubacterium C Purification and characterization of DNA polymerase III'. Identification of tau as a subunit of the DNA polymerase III holoenzyme. 4.1E+05 Dalton
Vaccinia Virus Pol Virus/Phage B Characterization of a processive form of the vaccinia virus DNA polymerase. 1.16E+05 Dalton
Dme Pol delta Eukaryote B Drosophila DNA polymerase delta. Purification and characterization. 1.85E+05 Dalton Technique: SDS-PAGE
ASFV pol X Virus/Phage X Characterization of an African swine fever virus 20-kDa DNA polymerase involved in DNA repair. 2E+04 Dalton
Stoffel fragment Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 6.13E+04 Dalton
SsoDpo1 Archaeon B Strand annealing and terminal transferase activities of a B-family DNA polymerase. 101kD Technique: SDS-PAGE
SsoDpo1 Archaeon B A trimeric DNA polymerase complex increases the native replication processivity. 1.01E+05 Dalton Technique: Gel Filtration
T7 (no thioreoxin) Virus/Phage A Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. 8E+04 Dalton Technique: SDS-PAGE
Sce Pol III Eukaryote C Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 125kD
Sce Pol II Eukaryote B Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 145kD
A Virus/Phage B Properties of the adenovirus DNA polymerase. 1.4E+05 Dalton
Sce Pol delta* Eukaryote B Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta. 180kD
AcNPV Virus/Phage B Autographa californica nuclear polyhedrosis virus DNA polymerase: measurements of processivity and strand displacement. 1.1E+05 Dalton
Sce Pol II* Eukaryote ? Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 200kD
Pab Pol I Archaeon B PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi. 84.8kD Technique: Sequence
MDV Virus/Phage B Identification and characterization of a Marek's disease virus gene encoding DNA polymerase. 1.35E+05 Dalton Technique: SDS-PAGE
Sce Pol I Eukaryote B Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. 1.8E+05 Dalton
Human Pol nu Eukaryote A Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol. 100kD
Bovine pol elta Eukaryote B Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure. 173kD Technique: Gel Filtration
Mouse pol elta Eukaryote ? Two forms of DNA polymerase delta from mouse cells. Purification and properties. 125kD Technique: SDS-PAGE
Mouse pol elta Eukaryote ? Mutation at the polymerase active site of mouse DNA polymerase delta increases genomic instability and accelerates tumorigenesis. 125kD
Cal pol gamma Eukaryote A Purification and characterization of a gamma-like DNA polymerase from Chenopodium album L. 8.5E+04 Dalton Technique: Gel Filtration
Eco Pol I - Small Fragment Eubacterium Non Pol Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 3.6E+04 Dalton
Eco Pol I - Small Fragment Eubacterium Non Pol Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 3.6E+04 Dalton
Eco Pol III* Eubacterium C A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties. 9E+04 Dalton Technique: Gel Filtration
Eco Pol III Holoenzyme Eubacterium C A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties. 3.3E+05 Dalton Technique: Gel Filtration
Eco Pol III Holoenzyme Eubacterium C ATP activation of DNA polymerase III holoenzyme of Escherichia coli. I. ATP-dependent formation of an initiation complex with a primed template. 1.9E+06 Dalton

Version:20241007

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