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Results for property: 5-3' exo

Detailed Results for Property

Use this page to examine all the the polymerases and references referring to the property: 5-3' exo.

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Polymerase Kingdom Family Reference Result Context
RB69 D222AD327A beta hairpin- Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Unspecified
Vent Archaeon B Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. No
Vent Archaeon B Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. No
Bst Eubacterium A Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity. Yes
9egN Archaeon B Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. No
Bsu (large fragment) Eubacterium ? ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS. No
Eco Pol I Eubacterium A The steady state kinetic parameters and non-processivity of Escherichia coli deoxyribonucleic acid polymerase I. Yes
Bst LF Eubacterium A Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity. No
T7 Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. Yes
Klenow fragment Eubacterium A Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. No
EBV Pol Virus/Phage B Characterization of 3'-to 5'-exonuclease activity associated with Epstein-Barr virus DNA polymerase. No
Tth Pol I Eubacterium A Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Yes
Tne pol Eubacterium A Domain exchange: chimeras of Thermus aquaticus DNA polymerase, Escherichia coli DNA polymerase I and Thermotoga neapolitana DNA polymerase. Yes
Thermo JDF-3 pol Archaeon B Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. No
Terminal Transferase Unknown ? Distinct and opposite diversifying activities of terminal transferase splice variants. Yes
Streptococcus pneumoniae Pol I Eubacterium A Characterization of the polA gene of Streptococcus pneumoniae and comparison of the DNA polymerase I it encodes to homologous enzymes from Escherichia coli and phage T7. Yes
T2 Virus/Phage B Separation of deoxyribo-oligonucleotidases induced by infection with bacteriophage T2. No
T5 Virus/Phage A Exonuclease associated with bacteriophage T5-Induced DNA polymerase. No
Streptococcus pneumoniae Pol I Eubacterium A The 5' to 3' exonuclease activity of DNA polymerase I is essential for Streptococcus pneumoniae. Yes
Streptococcus pneumoniae Pol I Eubacterium A Streptococcus pneumoniae DNA polymerase I lacks 3'-to-5' exonuclease activity: localization of the 5'-to-3' exonucleolytic domain. Yes
Sce Pol alpha Eukaryote B Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product. Yes
Pol eta Y52E Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. No
Human Pol beta Eukaryote X Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism. No
Pol eta Y52F Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. No
Pol eta Y52A Eukaryote Y Amino acid substitutions at conserved tyrosine 52 alter fidelity and bypass efficiency of human DNA polymerase eta. No
Bsu Pol X Eubacterium X Characterization of a Bacillus subtilis 64-kDa DNA polymerase X potentially involved in DNA repair. No
T4 Virus/Phage B The slow dissociation of the T4 DNA polymerase holoenzyme when stalled by nucleotide omission. An indication of a highly processive enzyme. Unspecified
Human Pol beta Eukaryote X Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme. No
Human Pol alpha Eukaryote B Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. No
Human Pol delta Eukaryote B Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. No
Human Pol epsilon Eukaryote B Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. No
Vent Archaeon B Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
Taq pol I Eubacterium A Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
PGBD Pol I Archaeon B Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
Pfu Archaeon B Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
T4 Virus/Phage B Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
Eco Pol I Eubacterium A Incorporation of reporter-labeled nucleotides by DNA polymerases. Unspecified
Taq (exo-) Eubacterium ? Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. No
Taq pol I Eubacterium A Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Yes
TaqTBD Eubacterium ? Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Yes
Vaccinia Virus Pol Virus/Phage B Characterization of a processive form of the vaccinia virus DNA polymerase. Yes
Dme Pol delta Eukaryote B Drosophila DNA polymerase delta. Purification and characterization. No
Human Pol eta Eukaryote Y Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta. Unspecified
Bst LF Eubacterium A Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Yes
Stoffel fragment Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. No
Taq pol I Eubacterium A High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Yes
Eco Pol I Eubacterium A Characterization of DNA polymerase I*, a form of DNA polymerase I found in Escherichia coli expressing SOS functions. Yes
Eco Pol I* Eubacterium A Characterization of DNA polymerase I*, a form of DNA polymerase I found in Escherichia coli expressing SOS functions. Yes
Human Pol eta Eukaryote Y Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta. Unspecified
HIV RT Virus/Phage RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. No
Eco Pol I Eubacterium A Short deoxyribonucleic acid repair patch length in Escherichia coli is determined by the processive mechanism of deoxyribonucleic acid polymerase I. Yes
T7 Virus/Phage A Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Yes
Eco Pol II Eubacterium B Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. Unspecified
Eco Pol I Eubacterium A Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins. Yes
Phi29 Virus/Phage B Functional importance of bacteriophage phi29 DNA polymerase residue Tyr148 in primer-terminus stabilisation at the 3'-5' exonuclease active site. Unspecified
Pfu Archaeon B The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases. Unspecified
Eco Pol I Eubacterium A Thermostable DNA polymerases. Yes
Human Pol epsilon Eukaryote B Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol epsilon and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors. Unspecified
Eco Pol II Eubacterium B Thermostable DNA polymerases. No
Eco Pol III Eubacterium C Thermostable DNA polymerases. No
Human Pol alpha Eukaryote B Thermostable DNA polymerases. No
Human Pol beta Eukaryote X Thermostable DNA polymerases. No
Human Pol delta Eukaryote B Thermostable DNA polymerases. No
Human Pol epsilon Eukaryote B Thermostable DNA polymerases. No
Taq pol I Eubacterium A Thermostable DNA polymerases. Yes
Sce Pol alpha Eukaryote B Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. No
Sce Pol II* Eukaryote ? Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. No
Human Pol gamma Eukaryote A Thermostable DNA polymerases. No
Klenow (exo-) Eubacterium A Kinetics of DNA polymerase I (Klenow fragment exo-) activity on damaged DNA templates: effect of proximal and distal template damage on DNA synthesis. Unspecified
Deep Vent (exo-) Archaeon B Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo- ) DNA polymerase. Unspecified
HHV1 YD12 Virus/Phage ? Finger domain mutation affects enzyme activity, DNA replication efficiency, and fidelity of an exonuclease-deficient DNA polymerase of herpes simplex virus type 1. Unspecified
HHV1 L774F Virus/Phage ? Finger domain mutation affects enzyme activity, DNA replication efficiency, and fidelity of an exonuclease-deficient DNA polymerase of herpes simplex virus type 1. Unspecified
HHV1 Virus/Phage B Finger domain mutation affects enzyme activity, DNA replication efficiency, and fidelity of an exonuclease-deficient DNA polymerase of herpes simplex virus type 1. Unspecified
TGO Archaeon B The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases. Unspecified
Klenow fragment Eubacterium A Identification of a new motif required for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I (Klenow fragment): the RRRY motif is necessary for the binding of single-stranded DNA substrate and the template strand of the mismatched duplex. Unspecified
Bsu Pol X Eubacterium X Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase. Unspecified
Klenow (exo-) Eubacterium A Synthesis of novel poly(dG)-poly(dG)-poly(dC) triplex structure by Klenow exo- fragment of DNA polymerase I. Unspecified
Klenow fragment Eubacterium A Synthesis of novel poly(dG)-poly(dG)-poly(dC) triplex structure by Klenow exo- fragment of DNA polymerase I. Unspecified
Klenow fragment Eubacterium A Contribution of polar residues of the J-helix in the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I (Klenow fragment): Q677 regulates the removal of terminal mismatch. Unspecified
Klenow (exo-) Eubacterium A In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I. Unspecified
Klenow fragment Eubacterium A In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I. No
Human Pol alpha Eukaryote B Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. No
Human Pol delta Eukaryote B Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. No
Human Pol epsilon Eukaryote B Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. No
Klenow fragment Eubacterium A The proofreading 3'-->5' exonuclease activity of DNA polymerases: a kinetic barrier to translesion DNA synthesis. No
Human Pol gamma Eukaryote A The proofreading 3'-->5' exonuclease activity of DNA polymerases: a kinetic barrier to translesion DNA synthesis. No
T4 Virus/Phage B The proofreading 3'-->5' exonuclease activity of DNA polymerases: a kinetic barrier to translesion DNA synthesis. No
Klenow fragment Eubacterium A Miscoding potential of the N2-ethyl-2'-deoxyguanosine DNA adduct by the exonuclease-free Klenow fragment of Escherichia coli DNA polymerase I. No
Klenow fragment Eubacterium A 3'-5' exonuclease of Klenow fragment: role of amino acid residues within the single-stranded DNA binding region in exonucleolysis and duplex DNA melting. No
Phi29 Virus/Phage B Phi29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal protein. Unspecified
T4 Virus/Phage B Architecture of the bacteriophage T4 replication complex revealed with nanoscale biopointers. No
Human Pol beta Eukaryote X DNA polymerase family X: function, structure, and cellular roles. No
Human Pol lamba Eukaryote X DNA polymerase family X: function, structure, and cellular roles. No
Human Pol mu Eukaryote X DNA polymerase family X: function, structure, and cellular roles. No
Dpo4 Archaeon Y Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. No
Sce Pol I Eukaryote B Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Unspecified
Sce Pol delta Eukaryote B The multiple biological roles of the 3'-->5' exonuclease of Saccharomyces cerevisiae DNA polymerase delta require switching between the polymerase and exonuclease domains. Unspecified
Bst Eubacterium A Thermostable DNA polymerases. Yes
Tma Pol III Eubacterium C Thermostable DNA polymerases. Yes
Poc Pol I Archaeon B The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases. Yes
Poc Pol II Archaeon B The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases. Yes
Pfu Archaeon B DNA polymerases from hyperthermophiles. No
Pfu (exo-) ? ? DNA polymerases from hyperthermophiles. No
Vent Archaeon B DNA polymerases from hyperthermophiles. No
PGBD Pol I Archaeon B DNA polymerases from hyperthermophiles. No
9egN Archaeon B DNA polymerases from hyperthermophiles. No
Taq pol I Eubacterium A DNA polymerases from hyperthermophiles. Yes
Bst LF Eubacterium A DNA polymerases from hyperthermophiles. No
Klenow fragment Eubacterium A DNA polymerases from hyperthermophiles. No
Cal pol gamma Eukaryote A Purification and characterization of a gamma-like DNA polymerase from Chenopodium album L. No
Pab Pol I Archaeon B PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi. No
Klenow Fragment D424A Eubacterium A Coordination between the polymerase and 5'-nuclease components of DNA polymerase I of Escherichia coli. No
Eco Pol I D424A Eubacterium A Coordination between the polymerase and 5'-nuclease components of DNA polymerase I of Escherichia coli. Yes
Eco Pol I - Small Fragment Eubacterium Non Pol Coordination between the polymerase and 5'-nuclease components of DNA polymerase I of Escherichia coli. Yes
Eco Pol I Exonuclease I Eubacterium Non Pol THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE. No
Eco Pol I Exonuclease II Eubacterium Non Pol THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE. No
Bsu Eubacterium ? ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS. Yes
Taq pol I Eubacterium A Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. Yes
Vaccinia Virus Pol Virus/Phage B Enzymatic processing of replication and recombination intermediates by the vaccinia virus DNA polymerase. No
Eco Pol I Eubacterium A ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Unspecified
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. No
Eco Pol I Exonuclease II Eubacterium Non Pol Enzymatic synthesis of deoxyribonucleic acid. XXIX. Hydrolysis of deoxyribonucleic acid from the 5' terminus by an exonuclease function of deoxyribonucleic acid polymerase. Yes
T4 D219A Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. Unspecified
T4 Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. Unspecified
Bsu Eubacterium ? ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. No
Eco Pol I Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Yes
Eco Pol I Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. XXXI. Binding of deoxyribonucleic acid to deoxyribonucleic acid polymerase. Unspecified
T4 Virus/Phage B Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. No
Eco Pol I Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. Yes
Eco Pol I Eubacterium A Enzymatic synthesis of deoxyribonucleic acid. XXXV. A 3'-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase. Yes
Klenow fragment Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. No
Eco Pol I - Small Fragment Eubacterium Non Pol Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Yes
Eco Pol I Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Yes
Eco Pol I - Small Fragment Eubacterium Non Pol Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Yes
Eco Pol I Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Yes
Klenow fragment Eubacterium A Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. No
Phi29 Virus/Phage B Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase. Unspecified
Phi29 Virus/Phage B Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding. Unspecified
Human Pol gamma D198AG200A Eukaryote A Human mitochondrial DNA polymerase γ exhibits potential for bypass and mutagenesis at UV-induced cyclobutane thymine dimers. Unspecified

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.