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Results for property: Nucleotide Substitution Rate

Detailed Results for Property

Use this page to examine all the the polymerases and references referring to the property: Nucleotide Substitution Rate.

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Polymerase Kingdom Family Reference Result Context
T4 Y254S Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 36 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254Q Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 47 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254K Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 77 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254L Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 7 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254G Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 72 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 113 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255A Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 107 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256Q Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256Y Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256K Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256L Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257S Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257Q Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 4 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257Y Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257L Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257G Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 6 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 I50L Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 64[KD]uplication Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 50 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 N84K Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
Taq pol I Eubacterium A Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. 8.9E-05 errors/bp
Taq pol I Eubacterium A Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. 0.00018 errors/bp Technique: Blue/white screening (0M Betaine, pWB407 template)
Taq pol I Eubacterium A Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. 0.00021 errors/bp Technique: Blue/white screening (1.3M Betaine, pWB407 template)
T4 D112AE114A Virus/Phage B Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. 300 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D324A Virus/Phage B Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. 60 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 4 Mutation frequency (relative to WT) Technique: ac forward mutational assay
Klenow fragment Eubacterium A Fidelity of DNA polymerases in DNA amplification. 0.00013 errors/bp Technique: DGGE
T4 Virus/Phage B Fidelity of DNA polymerases in DNA amplification. 3E-06 errors/bp Technique: DGGE
Sequenase Virus/Phage A Fidelity of DNA polymerases in DNA amplification. 3.4E-05 errors/bp Technique: DGGE
Taq pol I Eubacterium A Fidelity of DNA polymerases in DNA amplification. 0.00021 errors/bp Technique: DGGE
T4 D189A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 1 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 E191A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 5 Mutation frequency (relative to WT) Technique: ac forward mutational assay
Sequenase Virus/Phage A Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. 4.4E-05 errors/bp Technique: DGGE (2mM MgSO4)
Vent (exo-) Archaeon B Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. 0.00019 errors/bp Technique: Reversion (1mM dNTP)
Replinase Unknown ? Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. 0.000103 errors/bp Technique: Reversion (1mM dNTP)
T7 Virus/Phage A Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. 1.5E-05 errors/bp Technique: Reversion (1mM dNTP)
Vent Archaeon B Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. 5.8E-05 errors/bp Technique: Reversion (1mM dNTP)
Taq pol I Eubacterium A Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. 0.0002 errors/bp Technique: Sequencing
Klenow fragment Eubacterium A Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. 8E-05 errors/bp Technique: Sequencing
T4 D863up/S345F Virus/Phage B Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. 0.05 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (30°C)
T4 S345F Virus/Phage B Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. 0.02 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (30°C)
T4 Virus/Phage B On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. 1E-06 errors/bp Technique: Reversion (1 mM dNTP)
Eco Pol I Eubacterium A On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. 9.01E-06 errors/bp Technique: Reversion (1 mM dNTP)
Vent Archaeon B Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. 2.4E-05 errors/bp Technique: DGGE (2mM MgSO4)
Klentaq1 Eubacterium ? Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. 0.00015 errors/bp Technique: Blue/white screening (0M Betaine, pWB407 template)
Klentaq1 Eubacterium ? Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. 0.00012 errors/bp Technique: Blue/white screening (1.3M Betaine, pWB407 template)
Klentaq1 Eubacterium ? Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. 0.00013 errors/bp
T7 (purified without EDTA) Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. 0.045 errors/bp
Taq pol I Eubacterium A Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. 0.000111 errors/bp
T4 D863up/R335C Virus/Phage B Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (30°C)
Klenow (exo-) Eubacterium A Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. 1.4E-05 errors/bp Technique: Reversion (pH 6.2)
Klenow (exo-) Eubacterium A Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. 0.00076 errors/bp Technique: Reversion (pH 9.8)
T4 Virus/Phage B Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. 1E-06 errors/bp Technique: Gel kinetic assay (dCMP-A mismatch)
T4 Virus/Phage B Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. 1E-05 errors/bp Technique: Gel kinetic assay (dGMP-A mismatch)
T4 Virus/Phage B Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. 1E-08 errors/bp Technique: Gel kinetic assay (dAMP-A mismatch)
T4 Virus/Phage B Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. 0.0002 errors/bp Technique: Gel kinetic assay (dTMP-T mismatch)
T4 A89TD363N Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Spontaneous)
T4 A89TD363N Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (AP-induced)
T4 A89TD363N Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 3.8 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch)
T4 A737V Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 0.002 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (spontaneous)
T4 A737V Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 0.0025 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (AP-induced)
T4 A737V Virus/Phage B 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch)
T4 E191A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T7 Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. 0.028 errors/bp
T7 Virus/Phage A Two forms of the DNA polymerase of bacteriophage T7. 0.045 errors/bp
Klenow fragment Eubacterium A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 5.8E-06 errors/bp Technique: M13mp2 forward mutation assay (1 uM dNTP)
KF D424A Eubacterium A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 2.5E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results)
KF D355AE357A Eubacterium A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 2.5E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF(515-928) Eubacterium A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3.2E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP)
T4 E191A Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 6 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 D189AE191A Virus/Phage ? DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 16 Mutation frequency (relative to WT) Technique: ac forward mutational assay
Pfu Archaeon B High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. 1.6E-06 errors/bp
T4 P447L Virus/Phage B General antimutators are improbable. 1.3 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 W458S Virus/Phage B General antimutators are improbable. 1.4 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 R578op Virus/Phage B General antimutators are improbable. 1 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 Q592S Virus/Phage B General antimutators are improbable. 1.3 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 Q386Y Virus/Phage B General antimutators are improbable. 1.2 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 Q730S Virus/Phage B General antimutators are improbable. 1.4 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 Q731S Virus/Phage B General antimutators are improbable. 1.3 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 A737V Virus/Phage B General antimutators are improbable. 0.9 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 W202S Virus/Phage B General antimutators are improbable. 1.9 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 D219AD324A Virus/Phage B Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment. 310 Mutation frequency (relative to WT) Technique: Forward mutational
RB69 D222AD327A Virus/Phage B Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment. 490 Mutation frequency (relative to WT) Technique: Forward mutational
T4 M1I ? ? General antimutators are improbable. 1.8 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 G255S Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 58 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 271[L]uplication Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 74 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 V355A Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q380K Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E395K Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 14 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 9 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E743K Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S756P Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 13 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L771F Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 0.3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L771H Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 8 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 [L771+D] Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 29 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 [L771+V] Virus/Phage B Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. 22 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Virus/Phage B Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. 7.7 Mutation frequency (relative to WT) Technique: rII+ reversion (2AP (1 mg/ml))
T4 I417V Virus/Phage B Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. 0.1 Mutation frequency (relative to WT) Technique: rII+ reversion (2AP (1 mg/ml))
T4 R335C Virus/Phage B Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (30°C)
T4 D219A Virus/Phage B Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. 500 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A737V Virus/Phage B Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. 0.006 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412MA737V Virus/Phage B Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. 0.02 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255SA737V Virus/Phage B Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. 0.04 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Virus/Phage B Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. 9 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Virus/Phage B Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. 48 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 I417V Virus/Phage B Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. 0.004 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412MI417V Virus/Phage B Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. 0.025 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Virus/Phage B Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. 9.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412I Virus/Phage B Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. 0.09 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S411TL412M Virus/Phage B Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. 0.06 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189AE191A Virus/Phage ? DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189AE191A Virus/Phage ? DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 30 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 A89TD363N Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 74 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 36 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 280 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 D324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 63 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 56 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 E191AD324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 650 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 E191AD324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 514 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E191AD324G Virus/Phage B DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. 1800 errors/bp Technique: T4 rII P7oc reversion assay
T4 A89TD363N Virus/Phage B Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. 20 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D363N Virus/Phage B Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. 5 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 A89T Virus/Phage B Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. 8 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D219A Virus/Phage B Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. 760 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 Q592S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D112AE114A Virus/Phage B Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. 300 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Virus/Phage B Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. 58 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D131N Virus/Phage B Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D131G Virus/Phage B Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. 250 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
RB69 Y567A Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 820 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 Y567A Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 1400 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 Y567S Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 1200 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 Y567S Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 1600 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 Y567T Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 2600 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 Y567T Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 810 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 D222AD327A Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 600 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 D222AD327A Virus/Phage B Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 2600 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 L561A Virus/Phage B The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. 2.6 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 L561A Virus/Phage B The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. 4.3 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 0.6 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600))
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 0.2 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supD (CR63))
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1.2 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600))
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 2.2 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600))
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 0.1 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63))
T4 Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 0.4 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 238 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 538 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1189 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supE (MBM7007.2))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1744 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supD (MBM7007.1))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1980 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600))
T4 Q592K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 343 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 742 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600))
T4 L340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 941 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (AT->GC, Host: supD (CR63))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 844 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 674 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 2305 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supE (MBM7007.2))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 4160 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supD (MBM7007.1))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 480 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supE (C600))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 2500 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 500 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600))
T4 V325AL340P Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 642 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supD (CR63))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 6 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 4 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 2930 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supE (MBM7007.2))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 96 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supD (MBM7007.1))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1720 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1417 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1600 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600))
T4 L340PQ731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 20 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supD (CR63))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 35 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 17 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 4453 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supE (MBM7007.2))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 223 Mutation frequency (relative to WT) Technique: ac forward mutational assay (Host: supD (MBM7007.1))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 590 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1967 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 1867 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600))
T4 V325AL340P/Q731am Virus/Phage B Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. 49 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supD (CR63))
T4 L340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 2000 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C)
T4 L340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 333 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C)
T4 L340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 750 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 34 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 43°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 36 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 43°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 20 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 43°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 480 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 2500 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 500 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 214 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 37°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 333 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 37°C)
T4 V325AL340P Virus/Phage B Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. 36 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 37°C)
T4 A89TD363N Virus/Phage B Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. 80 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L340P Virus/Phage B Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. 55 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G82D Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 45 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 Y317C Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 410 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 L340P Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 210 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 S345P Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 25 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G694S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 3 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 L309P Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 716 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 V325A Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 105 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 N456TE881K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 320 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 Q478RA488V Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 103 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 W213Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W213S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W202Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W202Q Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W202S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q386S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 errors/bp Technique: T4 rII 199oc reversion assay
T4 Q386K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q386Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q430Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q430K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q430S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W458S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.05 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W458Q Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W458Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W458K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W482S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W482Q Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 6 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W482Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W482K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q730S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.001 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q730Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q730K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D131N Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 45 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D131G Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 125 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G255S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 328 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G298D Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 174 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G304D Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 154 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D131G Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 2728 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G82D Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 300 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G82D Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 92 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc -> P7am))
T4 G82D Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 100 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 G82D Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 112 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 L340P Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 380 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 L340P Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 320 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 L340P Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 1125 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 A89TD363N Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 90 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 A89TD363N Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 570 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 A89TD363N Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 1900 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 Y317C Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 800 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 Y317C Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 1720 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 Y317C Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 2110 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 S256G Virus/Phage B Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 34 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 P424L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.02 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 I50TP424L Virus/Phage ? Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 I50LP424L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 R335CP378L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.13 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 P378L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 R335CP424L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.03 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 R335C Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412MP424L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 P424I Virus/Phage ? Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 9 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 I50L Virus/Phage B Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W593S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W593Q Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W593Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 7 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W593K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W844S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.002 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 W844Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731S Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.004 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731Y Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731K Virus/Phage B Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 68 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 A89TD363N Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 A89TD363N Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 62 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 A89TD363N Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 254 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 27 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 G694S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 16 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 20 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 G694S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 17 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 Q731S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 0.001 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731Y Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 0.015 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 2.4 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731Y Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G694SQ731S Virus/Phage B Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. 0.12 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q478RA488V Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 266 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 Q478RA488V Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 200 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 Q478RA488V Virus/Phage B Isolation of bacteriophage T4 DNA polymerase mutator mutants. 188 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
Taq pol I Eubacterium A Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. 0.00192 errors/bp
TaqTBD Eubacterium ? Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. 0.00148 errors/bp
Sce Pol alpha Y951P Eukaryote B Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. 0.0034 errors/bp Technique: Forward mutational
Sce Pol alpha Eukaryote B Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. 0.0034 errors/bp Technique: Forward mutational
KF D424A Eubacterium A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 0.00012 errors/bp Technique: Reversion (1 uM dNTP)
Human Pol kappa Eukaryote Y Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 0.0058 errors/bp
Pol Kappa (exo-) Eukaryote Y Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 0.0074 errors/bp
T7 Virus/Phage A Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. 2.2E-06 errors/bp Technique: M13mp2 forward mutation assay
Klenow fragment Eubacterium A A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. 0.00036 errors/bp Technique: Reversion
Klenow fragment Eubacterium A A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. 0.0028 errors/bp Technique: Forward mutational
Human Pol lamba Eukaryote X DNA polymerase lambda, a novel DNA repair enzyme in human cells. 0.0009 errors/bp Technique: M13mp2 forward mutation assay
Human Pol alpha Eukaryote B Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. 1.3E-06 errors/bp Technique: Reversion
Human Pol alpha Eukaryote B Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. 1.1E-05 errors/bp Technique: Forward mutational
EIAV RT Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.003115 errors/bp
EIAV RT Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.001111 errors/bp
AMV Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.002193 errors/bp
AMV Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.000349 errors/bp
HIV RT Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.003155 errors/bp
HIV RT Virus/Phage RT Purification and partial characterization of equine infectious anemia virus reverse transcriptase. 0.001477 errors/bp
HIV RT Virus/Phage RT In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT. 6.3E-05 errors/bp
PFV RT Virus/Phage RT In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT. 5.8E-05 errors/bp
Human Pol gamma Eukaryote A The fidelity of human DNA polymerase gamma with and without exonucleolytic proofreading and the p55 accessory subunit. 1E-05 errors/bp
Human pol gamma (exo-) Eukaryote A The fidelity of human DNA polymerase gamma with and without exonucleolytic proofreading and the p55 accessory subunit. 4.5E-05 errors/bp
Klenow fragment Eubacterium A Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. 4E-05 errors/bp
HIV RT Virus/Phage RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 2.63E-05 errors/bp Technique: Reversion (pH 6.5)
HIV RT Virus/Phage RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 0.00027 errors/bp Technique: Reversion (pH 8.0)
Human Pol alpha Eukaryote B Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 2.38E-05 errors/bp Technique: Reversion (pH 6.1)
Human Pol alpha Eukaryote B Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 0.000154 errors/bp Technique: Reversion (pH 7.8)
Human Pol alpha Eukaryote B Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 0.000256 errors/bp Technique: Reversion (pH 8.6)
AMV Virus/Phage RT Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro. 2.7E-05 errors/bp Technique: M13mp2 opal codon reversion assay
Dpo4 Archaeon Y Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. 0.0065 errors/bp Technique: M13mp2 forward mutation assay
Chicken Pol beta Eukaryote X The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations. 0.019 errors/bp Technique: M13mp2 forward mutation assay
Rat Pol beta Eukaryote X The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations. 0.022 errors/bp Technique: M13mp2 forward mutation assay
Human Pol lamba Eukaryote X The frameshift infidelity of human DNA polymerase lambda. Implications for function. 0.0009 errors/bp Technique: M13mp2 forward mutation assay
Eco Pol III Eubacterium C The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. 8E-08 errors/bp
MutD5 Pol III Eubacterium C The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. 3.5E-06 errors/bp
MutD5 Pol III Eubacterium C The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. 44 Mutation frequency (relative to WT)
Human Pol eta Eukaryote Y Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 0.035 errors/bp
Human Pol beta Eukaryote X Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 0.00067 errors/bp
Human Pol alpha Eukaryote B Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 0.00016 errors/bp
Human Pol delta Eukaryote B Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 1E-05 errors/bp
Mouse Pol eta Eukaryote Y Error rate and specificity of human and murine DNA polymerase eta. 0.022 errors/bp Technique: M13mp2 forward mutation assay
Human Pol eta Eukaryote Y Error rate and specificity of human and murine DNA polymerase eta. 0.032 errors/bp Technique: M13mp2 forward mutation assay
Eco Pol IV Eubacterium Y Fidelity of Escherichia coli DNA polymerase IV. Preferential generation of small deletion mutations by dNTP-stabilized misalignment. 5.1E-05 errors/bp Technique: M13mp2 forward mutation assay
Eco Pol V Eubacterium Y Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis. 0.00019 errors/bp
Eco Pol III Eubacterium C Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis. 3.8E-06 errors/bp
Sce Pol I Eukaryote B Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. 8.33E-05 errors/bp Technique: M13mp2 opal codon reversion assay
Human Pol eta Eukaryote Y Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. 0.0357 errors/bp Technique: Forward mutational
Human Pol kappa Eukaryote Y Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. 0.0066 errors/bp
Human Pol nu Eukaryote A A unique error signature for human DNA polymerase nu. 0.0035 errors/bp Technique: M13mp2 forward mutation assay
Human Pol theta Eukaryote A Low-fidelity DNA synthesis by human DNA polymerase theta. 0.0024 errors/bp Technique: M13mp2 forward mutation assay
Taq pol I Eubacterium A High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. 0.0003125 errors/bp Technique: M13mp2 opal codon reversion assay (Low fidelity conditions)
Taq pol I Eubacterium A High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. 3.33E-06 errors/bp Technique: M13mp2 opal codon reversion assay (High fidelity conditions)
Human Pol eta Eukaryote Y The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. 0.035 errors/bp Technique: M13mp2 forward mutation assay
Sce Pol eta Eukaryote Y The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. 0.0095 errors/bp Technique: M13mp2 forward mutation assay
Sce Pol delta Eukaryote B The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. 1.3E-05 errors/bp Technique: M13mp2 forward mutation assay
Human Pol gamma Eukaryote A The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. 1E-05 errors/bp Technique: M13mp2 forward mutation assay
Human Pol kappa Eukaryote Y The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. 0.0058 errors/bp Technique: M13mp2 forward mutation assay
T4 D219A Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. 4E-05 errors/bp Technique: M13mp2 forward mutation assay
T4 D219A Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. 0.0001 errors/bp Technique: M13mp2 opal codon reversion assay
T4 Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. 9.4E-07 errors/bp Technique: M13mp2 forward mutation assay
T4 Virus/Phage B Effect of accessory proteins on T4 DNA polymerase replication fidelity. 3.3E-06 errors/bp Technique: M13mp2 opal codon reversion assay
T4 D863 uplication Virus/Phage B Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. 10 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (30°C)

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