| T7 |
Virus/Phage |
A
|
Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. |
2.2E-06 errors/bp |
Technique: M13mp2 forward mutation assay |
| T7 |
Virus/Phage |
A
|
Two forms of the DNA polymerase of bacteriophage T7. |
0.045 errors/bp |
|
| T7 |
Virus/Phage |
A
|
Two forms of the DNA polymerase of bacteriophage T7. |
0.028 errors/bp |
|
| T7 |
Virus/Phage |
A
|
Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. |
1.5E-05 errors/bp |
Technique: Reversion (1mM dNTP) |
| Vent |
Archaeon |
B
|
Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. |
2.4E-05 errors/bp |
Technique: DGGE (2mM MgSO4) |
| Vent |
Archaeon |
B
|
Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. |
5.8E-05 errors/bp |
Technique: Reversion (1mM dNTP) |
| T4 D219A |
Virus/Phage |
B
|
Effect of accessory proteins on T4 DNA polymerase replication fidelity. |
0.0001 errors/bp |
Technique: M13mp2 opal codon reversion assay |
| T4 D219A |
Virus/Phage |
B
|
Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. |
760 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D219A |
Virus/Phage |
B
|
Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. |
500 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D219A |
Virus/Phage |
B
|
Effect of accessory proteins on T4 DNA polymerase replication fidelity. |
4E-05 errors/bp |
Technique: M13mp2 forward mutation assay |
| T4 |
Virus/Phage |
B
|
Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. |
1E-08 errors/bp |
Technique: Gel kinetic assay (dAMP-A mismatch) |
| T4 |
Virus/Phage |
B
|
Fidelity of DNA polymerases in DNA amplification. |
3E-06 errors/bp |
Technique: DGGE |
| T4 |
Virus/Phage |
B
|
Effect of accessory proteins on T4 DNA polymerase replication fidelity. |
3.3E-06 errors/bp |
Technique: M13mp2 opal codon reversion assay |
| T4 |
Virus/Phage |
B
|
Effect of accessory proteins on T4 DNA polymerase replication fidelity. |
9.4E-07 errors/bp |
Technique: M13mp2 forward mutation assay |
| T4 |
Virus/Phage |
B
|
On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. |
1E-06 errors/bp |
Technique: Reversion (1 mM dNTP) |
| T4 |
Virus/Phage |
B
|
Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. |
1E-06 errors/bp |
Technique: Gel kinetic assay (dCMP-A mismatch) |
| T4 |
Virus/Phage |
B
|
Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. |
1E-05 errors/bp |
Technique: Gel kinetic assay (dGMP-A mismatch) |
| T4 |
Virus/Phage |
B
|
Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. |
0.0002 errors/bp |
Technique: Gel kinetic assay (dTMP-T mismatch) |
| T4 Y254S |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
36 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| RB69 D222AD327A |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
2600 Mutation frequency (relative to WT) |
Technique: RB69 rII UV375 reversion |
| RB69 D222AD327A |
Virus/Phage |
B
|
Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment. |
490 Mutation frequency (relative to WT) |
Technique: Forward mutational |
| RB69 D222AD327A |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
600 Mutation frequency (relative to WT) |
Technique: RB69 rII UV256 reversion |
| T4 W213S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 I417V |
Virus/Phage |
B
|
Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. |
0.1 Mutation frequency (relative to WT) |
Technique: rII+ reversion (2AP (1 mg/ml)) |
| T4 I417V |
Virus/Phage |
B
|
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. |
0.004 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A737V |
Virus/Phage |
B
|
General antimutators are improbable. |
0.9 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 A737V |
Virus/Phage |
B
|
Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. |
0.006 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A737V |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
0.0025 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (AP-induced) |
| T4 A737V |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch) |
| T4 A737V |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
0.002 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (spontaneous) |
| T4 I50L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 I50L |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 64[KD]uplication |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
50 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 N84K |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 271[L]uplication |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
74 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 V355A |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
5 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q380K |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
5 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 E395K |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
14 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412M |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
9 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412M |
Virus/Phage |
B
|
Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. |
7.7 Mutation frequency (relative to WT) |
Technique: rII+ reversion (2AP (1 mg/ml)) |
| T4 L412M |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
9 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412M |
Virus/Phage |
B
|
Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. |
9 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412M |
Virus/Phage |
B
|
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. |
9.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 E743K |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
11 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S756P |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
13 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L771F |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
0.3 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L771H |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
8 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 [L771+D] |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
29 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 [L771+V] |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
22 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255S |
Virus/Phage |
B
|
Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. |
58 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
328 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G255S |
Virus/Phage |
B
|
Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. |
58 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255S |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
113 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255S |
Virus/Phage |
B
|
Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. |
48 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255A |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
107 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Y254Q |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
47 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Y254K |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
77 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Y254L |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
7 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Y254G |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
72 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S256Y |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S256K |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
11 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S256Q |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S256L |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
15 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S256G |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
34 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 K257S |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 K257Q |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
4 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 K257Y |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
3 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 K257L |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
3 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 K257G |
Virus/Phage |
B
|
Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. |
6 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D112AE114A |
Virus/Phage |
B
|
Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. |
300 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D112AE114A |
Virus/Phage |
B
|
Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. |
300 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D324A |
Virus/Phage |
B
|
Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. |
60 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| Eco Pol I |
Eubacterium |
A
|
On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. |
9.01E-06 errors/bp |
Technique: Reversion (1 mM dNTP) |
| Klenow fragment |
Eubacterium |
A
|
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. |
8E-05 errors/bp |
Technique: Sequencing |
| Klenow fragment |
Eubacterium |
A
|
A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. |
0.00036 errors/bp |
Technique: Reversion |
| Klenow fragment |
Eubacterium |
A
|
A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. |
0.0028 errors/bp |
Technique: Forward mutational |
| Klenow fragment |
Eubacterium |
A
|
Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. |
4E-05 errors/bp |
|
| Klenow fragment |
Eubacterium |
A
|
Fidelity of DNA polymerases in DNA amplification. |
0.00013 errors/bp |
Technique: DGGE |
| Klenow fragment |
Eubacterium |
A
|
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. |
5.8E-06 errors/bp |
Technique: M13mp2 forward mutation assay (1 uM dNTP) |
| T4 L412I |
Virus/Phage |
B
|
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. |
0.09 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 R335C |
Virus/Phage |
B
|
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (30°C) |
| T4 R335C |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D131N |
Virus/Phage |
B
|
Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. |
15 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D131N |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
45 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D131G |
Virus/Phage |
B
|
Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. |
250 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D131G |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
2728 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D131G |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
125 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G82D |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
45 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G82D |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
112 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
| T4 G82D |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
300 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G82D |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
| T4 G82D |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
92 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC (P7oc -> P7am)) |
| T4 W202S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W202S |
Virus/Phage |
B
|
General antimutators are improbable. |
1.9 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 G298D |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
174 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G304D |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
154 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 L309P |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
716 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 Y317C |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
2110 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
| T4 Y317C |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
410 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 Y317C |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
800 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
| T4 Y317C |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
1720 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
| T4 D324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
56 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
280 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay |
| T4 D324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
63 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 V325A |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
105 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 L340P |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
320 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
| T4 L340P |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
1125 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1189 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supE (MBM7007.2)) |
| T4 L340P |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
210 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1744 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supD (MBM7007.1)) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
742 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600)) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
941 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (AT->GC, Host: supD (CR63)) |
| T4 L340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
2000 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
238 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63)) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
538 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63)) |
| T4 L340P |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
380 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
| T4 L340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
333 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C) |
| T4 L340P |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. |
55 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1980 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600)) |
| T4 L340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
343 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600)) |
| T4 L340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
750 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C) |
| T4 S345P |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
25 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 Q386S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 errors/bp |
Technique: T4 rII 199oc reversion assay |
| T4 P447L |
Virus/Phage |
B
|
General antimutators are improbable. |
1.3 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 W458S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.05 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W458S |
Virus/Phage |
B
|
General antimutators are improbable. |
1.4 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 W482S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 R578op |
Virus/Phage |
B
|
General antimutators are improbable. |
1 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 Q592S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q592S |
Virus/Phage |
B
|
General antimutators are improbable. |
1.3 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 W593S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G694S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
3 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 G694S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
16 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+2)) |
| T4 G694S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
27 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+1)) |
| T4 G694S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
17 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+2)) |
| T4 G694S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
20 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+3)) |
| T4 Q730S |
Virus/Phage |
B
|
General antimutators are improbable. |
1.4 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 Q730S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.001 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.004 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
0.001 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731S |
Virus/Phage |
B
|
General antimutators are improbable. |
1.3 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 W844S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.002 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D863 uplication |
Virus/Phage |
B
|
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. |
10 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (30°C) |
| T4 Q430S |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412MA737V |
Virus/Phage |
B
|
Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. |
0.02 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G255SA737V |
Virus/Phage |
B
|
Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. |
0.04 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| Dpo4 |
Archaeon |
Y
|
Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. |
0.0065 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol eta |
Eukaryote |
Y
|
Error rate and specificity of human and murine DNA polymerase eta. |
0.032 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol eta |
Eukaryote |
Y
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
0.035 errors/bp |
|
| Human Pol eta |
Eukaryote |
Y
|
Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. |
0.0357 errors/bp |
Technique: Forward mutational |
| Human Pol eta |
Eukaryote |
Y
|
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. |
0.035 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol gamma |
Eukaryote |
A
|
The fidelity of human DNA polymerase gamma with and without exonucleolytic proofreading and the p55 accessory subunit. |
1E-05 errors/bp |
|
| Human Pol gamma |
Eukaryote |
A
|
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. |
1E-05 errors/bp |
Technique: M13mp2 forward mutation assay |
| Eco Pol IV |
Eubacterium |
Y
|
Fidelity of Escherichia coli DNA polymerase IV. Preferential generation of small deletion mutations by dNTP-stabilized misalignment. |
5.1E-05 errors/bp |
Technique: M13mp2 forward mutation assay |
| Eco Pol V |
Eubacterium |
Y
|
Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis. |
0.00019 errors/bp |
|
| Human Pol kappa |
Eukaryote |
Y
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
0.0058 errors/bp |
|
| Human Pol kappa |
Eukaryote |
Y
|
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. |
0.0058 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol kappa |
Eukaryote |
Y
|
Functions of human DNA polymerases eta, kappa and iota suggested by their properties, including fidelity with undamaged DNA templates. |
0.0066 errors/bp |
|
| Human Pol beta |
Eukaryote |
X
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
0.00067 errors/bp |
|
| Rat Pol beta |
Eukaryote |
X
|
The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations. |
0.022 errors/bp |
Technique: M13mp2 forward mutation assay |
| Taq pol I |
Eubacterium |
A
|
Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. |
8.9E-05 errors/bp |
|
| Taq pol I |
Eubacterium |
A
|
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. |
0.0002 errors/bp |
Technique: Sequencing |
| Taq pol I |
Eubacterium |
A
|
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. |
0.00021 errors/bp |
Technique: Blue/white screening (1.3M Betaine, pWB407 template) |
| Taq pol I |
Eubacterium |
A
|
High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. |
3.33E-06 errors/bp |
Technique: M13mp2 opal codon reversion assay (High fidelity conditions) |
| Taq pol I |
Eubacterium |
A
|
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. |
0.00018 errors/bp |
Technique: Blue/white screening (0M Betaine, pWB407 template) |
| Taq pol I |
Eubacterium |
A
|
Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. |
0.00192 errors/bp |
|
| Taq pol I |
Eubacterium |
A
|
Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. |
0.000111 errors/bp |
|
| Taq pol I |
Eubacterium |
A
|
Fidelity of DNA polymerases in DNA amplification. |
0.00021 errors/bp |
Technique: DGGE |
| Taq pol I |
Eubacterium |
A
|
High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. |
0.0003125 errors/bp |
Technique: M13mp2 opal codon reversion assay (Low fidelity conditions) |
| HIV RT |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.001477 errors/bp |
|
| HIV RT |
Virus/Phage |
RT
|
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. |
2.63E-05 errors/bp |
Technique: Reversion (pH 6.5) |
| HIV RT |
Virus/Phage |
RT
|
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. |
0.00027 errors/bp |
Technique: Reversion (pH 8.0) |
| HIV RT |
Virus/Phage |
RT
|
In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT. |
6.3E-05 errors/bp |
|
| HIV RT |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.003155 errors/bp |
|
| Pfu |
Archaeon |
B
|
High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. |
1.6E-06 errors/bp |
|
| Eco Pol III |
Eubacterium |
C
|
The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. |
8E-08 errors/bp |
|
| Eco Pol III |
Eubacterium |
C
|
Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis. |
3.8E-06 errors/bp |
|
| Human Pol lamba |
Eukaryote |
X
|
DNA polymerase lambda, a novel DNA repair enzyme in human cells. |
0.0009 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol lamba |
Eukaryote |
X
|
The frameshift infidelity of human DNA polymerase lambda. Implications for function. |
0.0009 errors/bp |
Technique: M13mp2 forward mutation assay |
| Klenow (exo-) |
Eubacterium |
A
|
Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. |
0.00076 errors/bp |
Technique: Reversion (pH 9.8) |
| Klenow (exo-) |
Eubacterium |
A
|
Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. |
1.4E-05 errors/bp |
Technique: Reversion (pH 6.2) |
| Vent (exo-) |
Archaeon |
B
|
Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. |
0.00019 errors/bp |
Technique: Reversion (1mM dNTP) |
| Replinase |
Unknown |
?
|
Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. |
0.000103 errors/bp |
Technique: Reversion (1mM dNTP) |
| Klentaq1 |
Eubacterium |
?
|
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. |
0.00013 errors/bp |
|
| Klentaq1 |
Eubacterium |
?
|
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. |
0.00015 errors/bp |
Technique: Blue/white screening (0M Betaine, pWB407 template) |
| Klentaq1 |
Eubacterium |
?
|
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. |
0.00012 errors/bp |
Technique: Blue/white screening (1.3M Betaine, pWB407 template) |
| T4 A89T |
Virus/Phage |
B
|
Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. |
8 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| Sequenase |
Virus/Phage |
A
|
Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. |
4.4E-05 errors/bp |
Technique: DGGE (2mM MgSO4) |
| Sequenase |
Virus/Phage |
A
|
Fidelity of DNA polymerases in DNA amplification. |
3.4E-05 errors/bp |
Technique: DGGE |
| AMV |
Virus/Phage |
RT
|
Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro. |
2.7E-05 errors/bp |
Technique: M13mp2 opal codon reversion assay |
| AMV |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.000349 errors/bp |
|
| AMV |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.002193 errors/bp |
|
| RB69 Y567A |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
820 Mutation frequency (relative to WT) |
Technique: RB69 rII UV256 reversion |
| RB69 Y567A |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
1400 Mutation frequency (relative to WT) |
Technique: RB69 rII UV375 reversion |
| RB69 L561A |
Virus/Phage |
B
|
The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. |
2.6 Mutation frequency (relative to WT) |
Technique: RB69 rII UV256 reversion |
| RB69 L561A |
Virus/Phage |
B
|
The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. |
4.3 Mutation frequency (relative to WT) |
Technique: RB69 rII UV375 reversion |
| T4 A89TD363N |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
90 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
| T4 A89TD363N |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
254 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+2)) |
| T4 A89TD363N |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
62 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+3)) |
| T4 A89TD363N |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+2)) |
| T4 A89TD363N |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
68 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Host: B40(sup+1)) |
| T4 A89TD363N |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. |
80 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A89TD363N |
Virus/Phage |
B
|
Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. |
20 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 A89TD363N |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
36 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 A89TD363N |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
74 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A89TD363N |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
1900 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
| T4 A89TD363N |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
3.8 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch) |
| T4 A89TD363N |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
5 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (AP-induced) |
| T4 A89TD363N |
Virus/Phage |
B
|
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. |
100 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (Spontaneous) |
| T4 A89TD363N |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
570 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
0.6 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
0.4 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supD (CR63)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1.2 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600)) |
| T4 Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
2.2 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600)) |
| Human Pol alpha |
Eukaryote |
B
|
Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. |
1.1E-05 errors/bp |
Technique: Forward mutational |
| Human Pol alpha |
Eukaryote |
B
|
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. |
0.000256 errors/bp |
Technique: Reversion (pH 8.6) |
| Human Pol alpha |
Eukaryote |
B
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
0.00016 errors/bp |
|
| Human Pol alpha |
Eukaryote |
B
|
Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. |
1.3E-06 errors/bp |
Technique: Reversion |
| Human Pol alpha |
Eukaryote |
B
|
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. |
0.000154 errors/bp |
Technique: Reversion (pH 7.8) |
| Human Pol alpha |
Eukaryote |
B
|
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. |
2.38E-05 errors/bp |
Technique: Reversion (pH 6.1) |
| T7 (purified without EDTA) |
Virus/Phage |
A
|
Two forms of the DNA polymerase of bacteriophage T7. |
0.045 errors/bp |
|
| Human Pol delta |
Eukaryote |
B
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
1E-05 errors/bp |
|
| KF D424A |
Eubacterium |
A
|
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. |
0.00012 errors/bp |
Technique: Reversion (1 uM dNTP) |
| KF D424A |
Eubacterium |
A
|
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. |
2.5E-05 errors/bp |
Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results) |
| KF D355AE357A |
Eubacterium |
A
|
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. |
2.5E-05 errors/bp |
Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results) |
| KF(515-928) |
Eubacterium |
A
|
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. |
3.2E-05 errors/bp |
Technique: M13mp2 forward mutation assay (1mM dNTP) |
| T4 Q386Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q386Y |
Virus/Phage |
B
|
General antimutators are improbable. |
1.2 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 D219AD324A |
Virus/Phage |
B
|
Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment. |
310 Mutation frequency (relative to WT) |
Technique: Forward mutational |
| T4 M1I |
? |
?
|
General antimutators are improbable. |
1.8 Mutation frequency (relative to WT) |
Technique: Forward mutational (r+ -> r) |
| T4 D863up/R335C |
Virus/Phage |
B
|
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (30°C) |
| T4 D863up/S345F |
Virus/Phage |
B
|
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. |
0.05 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (30°C) |
| T4 S345F |
Virus/Phage |
B
|
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid. |
0.02 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay (30°C) |
| T4 L412MI417V |
Virus/Phage |
B
|
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. |
0.025 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 S411TL412M |
Virus/Phage |
B
|
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases. |
0.06 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D189A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay |
| T4 D189A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 D189A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
4 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 E191A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 E191A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
6 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay |
| T4 E191A |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
5 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D189AE191A |
Virus/Phage |
?
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
30 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay |
| T4 D189AE191A |
Virus/Phage |
?
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
16 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 D189AE191A |
Virus/Phage |
?
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
11 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 E191AD324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
514 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 E191AD324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
650 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 E191AD324G |
Virus/Phage |
B
|
DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. |
1800 errors/bp |
Technique: T4 rII P7oc reversion assay |
| T4 D363N |
Virus/Phage |
B
|
Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain. |
5 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| Sce Pol eta |
Eukaryote |
Y
|
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. |
0.0095 errors/bp |
Technique: M13mp2 forward mutation assay |
| Sce Pol alpha |
Eukaryote |
B
|
Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. |
0.0034 errors/bp |
Technique: Forward mutational |
| Sce Pol delta |
Eukaryote |
B
|
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. |
1.3E-05 errors/bp |
Technique: M13mp2 forward mutation assay |
| Human Pol theta |
Eukaryote |
A
|
Low-fidelity DNA synthesis by human DNA polymerase theta. |
0.0024 errors/bp |
Technique: M13mp2 forward mutation assay |
| RB69 Y567S |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
1600 Mutation frequency (relative to WT) |
Technique: RB69 rII UV375 reversion |
| RB69 Y567S |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
1200 Mutation frequency (relative to WT) |
Technique: RB69 rII UV256 reversion |
| RB69 Y567T |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
2600 Mutation frequency (relative to WT) |
Technique: RB69 rII UV256 reversion |
| RB69 Y567T |
Virus/Phage |
B
|
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. |
810 Mutation frequency (relative to WT) |
Technique: RB69 rII UV375 reversion |
| T4 P424L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.02 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
6 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
20 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supD (CR63)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1600 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1417 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1720 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
96 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supD (MBM7007.1)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
2930 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supE (MBM7007.2)) |
| T4 L340PQ731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
4 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
34 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 43°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
333 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 37°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
844 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
674 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
480 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supE (C600)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
36 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 37°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
642 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supD (CR63)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
500 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
2500 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
2305 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supE (MBM7007.2)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
4160 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supD (MBM7007.1)) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
214 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 37°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
500 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
2500 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
480 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
20 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 43°C) |
| T4 V325AL340P |
Virus/Phage |
B
|
Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. |
36 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 43°C) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
17 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supD (CR63)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
35 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supD (CR63)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
4453 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supE (MBM7007.2)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
223 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay (Host: supD (MBM7007.1)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
590 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC, Host: supE (C600)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1967 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG), Host: supE (C600)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
1867 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG), Host: supE (C600)) |
| T4 V325AL340P/Q731am |
Virus/Phage |
B
|
Structure-function studies of the bacteriophage T4 DNA polymerase. Isolation of a novel suppressor mutant. |
49 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay ( AT->GC, Host: supD (CR63)) |
| T4 N456TE881K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
320 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 Q478RA488V |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
188 Mutation frequency (relative to WT) |
Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
| T4 Q478RA488V |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
266 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
| T4 Q478RA488V |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
103 Mutation frequency (relative to WT) |
Technique: ac forward mutational assay |
| T4 Q478RA488V |
Virus/Phage |
B
|
Isolation of bacteriophage T4 DNA polymerase mutator mutants. |
200 Mutation frequency (relative to WT) |
Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
| T4 W202Q |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W202Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W213Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.15 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q386K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q430Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q430K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W458Q |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W458Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W458K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W482Q |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
6 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W482Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W482K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q592K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W593Q |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W593Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
7 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W593K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q730Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q730K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731Y |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
0.015 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 Q731K |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.01 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 W844Y |
Virus/Phage |
B
|
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 G694SQ731S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
0.12 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A89TD363N/Q731S |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
2.4 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 A89TD363N/Q731Y |
Virus/Phage |
B
|
Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. |
15 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| Sce Pol alpha Y951P |
Eukaryote |
B
|
Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. |
0.0034 errors/bp |
Technique: Forward mutational |
| T4 P378L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
5 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 P424I |
Virus/Phage |
?
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 I50TP424L |
Virus/Phage |
?
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 I50LP424L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.2 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 R335CP378L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.13 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 L412MP424L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.1 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| T4 R335CP424L |
Virus/Phage |
B
|
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. |
0.03 Mutation frequency (relative to WT) |
Technique: T4 rII 199oc reversion assay |
| TaqTBD |
Eubacterium |
?
|
Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. |
0.00148 errors/bp |
|
| Pol Kappa (exo-) |
Eukaryote |
Y
|
Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. |
0.0074 errors/bp |
|
| EIAV RT |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.003115 errors/bp |
|
| EIAV RT |
Virus/Phage |
RT
|
Purification and partial characterization of equine infectious anemia virus reverse transcriptase. |
0.001111 errors/bp |
|
| PFV RT |
Virus/Phage |
RT
|
In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT. |
5.8E-05 errors/bp |
|
| Human pol gamma (exo-) |
Eukaryote |
A
|
The fidelity of human DNA polymerase gamma with and without exonucleolytic proofreading and the p55 accessory subunit. |
4.5E-05 errors/bp |
|
| Chicken Pol beta |
Eukaryote |
X
|
The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations. |
0.019 errors/bp |
Technique: M13mp2 forward mutation assay |
| MutD5 Pol III |
Eubacterium |
C
|
The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. |
44 Mutation frequency (relative to WT) |
|
| MutD5 Pol III |
Eubacterium |
C
|
The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. |
3.5E-06 errors/bp |
|
| Mouse Pol eta |
Eukaryote |
Y
|
Error rate and specificity of human and murine DNA polymerase eta. |
0.022 errors/bp |
Technique: M13mp2 forward mutation assay |
| Sce Pol I |
Eukaryote |
B
|
Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. |
8.33E-05 errors/bp |
Technique: M13mp2 opal codon reversion assay |
| Human Pol nu |
Eukaryote |
A
|
A unique error signature for human DNA polymerase nu. |
0.0035 errors/bp |
Technique: M13mp2 forward mutation assay |
